In the present study, sulphated polysaccharide Ulvan from Ulva lactuca was used for the synthesis of biogenic Selenium Nanoparticles (SeNPs) conjugate and Mouth rinse was prepared using this conjugate. The synthesis of nanoparticles was confirmed by UV–Visible spectrophotometry and characterized using Fourier transform infrared spectroscopy (FTIR), transmission electron microscope (TEM) and X-ray diffraction (XRD). TEM showed that the average size of the nanoparticle was 85 nm and spherical in shape. Furthermore, nanoparticle conjugates were evaluated for cell viability using MTT assay 3T3-L1 cell line and at 30 µl/ml showed 34% cell viability. The antimicrobial activity of SeNPs mouth rinse was tested against oral pathogens such as Streptococcus mutans, Staphylococcus aureus, Lactobacillus, and Candida albicans and it was effective against all tested microorganism at the concentration of 100 µl/ml. The present study has shown that Ulvan from algal biomass can be a safe and effective source for the development of oral nano-antimicrobial agents.

The antibacterial activity of an Ulvan-based silver nanoparticle (AgNP) system was evaluated in the current study. The green synthesis of biogenic silver nanoparticles was conducted using Ulvan, a sulphated polysaccharide extracted from Ulva lactuca. A novel mouthwash containing AgNPs was prepared, and tested for its efficacy and safety. AgNPs were confirmed with spectrophotometric analysis (UV–A visible spectrophotometer), and the characterisation was established with Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and transmission electron microscopy (TEM). The AgNPs were spherical, and their average size was 8–33 nm, as shown via TEM. The antioxidant assay was conducted via DDPH assay, wherein the AgNPs, at a concentration of 50 μL/mL, showed 93.15% inhibition. Furthermore, anticancer activity was tested by evaluating the cell viability utilising the method of an MTT assay on the 3T3-L1 cell lines. AgNPs, at 30 µL/mL, showed maximal cell viability, denoting no cytotoxic effect. The silver-nanoparticle-based mouthrinse, at a concentration of 100 µL/mL, demonstrated antimicrobial activity against Streptococcus mutans, Staphylococcus aureus, Lactobacillus, and Candida albicans. This study shows that mouthwash prepared from the Ulvan-silver nanoparticle system could be a nontoxic and effective oral antimicrobial agent.

Context: Early Childhood Caries (ECC) is the most virulent form of caries, which requires early intervention. Pediatricians are health professionals who first examine the child, and must be able to educate parents about basic preventive dental care, early diagnosis of pathological changes in the oral cavity and appropriate referrals for the children. Aims: To assess the awareness, attitude, and practice among pediatricians regarding ECC and Infant Oral Healthcare (IOHC) in Puducherry. Settings and Design: Setting: Study was conducted among pediatricians of Puducherry. Design: Observational Cross-sectional study. Methods and Material: After obtaining consent from Pediatricians, a prevalidated questionnaire was distributed and collected from them regarding their awareness, attitude, and practices toward ECC and IOHC. Statistical Analysis Used: Percentage. Results: Out of 85% Pediatricians who responded, 57% of them were aware of initial white spot lesions, and 39% of them were aware of maternally-derived Streptococcus mutans disease. 100% of them were willing to avail information, training on IOHC. Conclusions: It is clear from the study that Pediatricians in Puducherry had limited awareness regarding the prevention of oral diseases. It is essential to provide sufficient knowledge to them by incorporating a module on ECC and infant oral health care in their curriculum.

To assess the effectiveness of visual distraction with and without virtual reality glasses in reducing dental anxiety among children with hearing and speech disabilities undergoing dental treatment. Twenty-four children with hearing and speech disabilities aged 6-12 years were selected and were randomly divided into three groups (N = 8). Children in group A received no distraction, group B received visual distraction using virtual reality (VR) glasses and group C received visual distraction without VR glasses during dental treatment. The anxiety levels were measured using PJS- Pictorial Scale and physiological parameters - before, during, and after a dental procedure. Then intragroup and intergroup comparison was done. Intragroup comparison showed that "During" and "Post" treatment anxiety scores were significantly (p < 0.05) lower than that of "baseline" in group B. Intergroup comparison of anxiety scores in the three groups, at all three intervals, showed a statistically significant difference in the "during treatment" anxiety score (p = 0.049) with least score in group B. Visual distraction using VR glasses can be recommended as an effective distraction technique in reducing dental anxiety among children with speech and hearing disabilities. Kaur J, Shivashankarappa PG, A S, et al. Effectiveness of Visual Distraction with and without Virtual Reality Glasses in Reducing Dental Anxiety among Children with Hearing and Speech Disability: A Pilot Study. Int J Clin Pediatr Dent 2021;14(S-2):S162-S166.