Gary Alan Flory

Mr. Gary Alan Flory


Research Focus & Metrics

Academic Career Length

4Years (2023-Present)

(Calculated once Scilit publication data is synced: current year − year of first publication + 1)

Representative Publications

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10 May 2024 in Pathogens
Verified
Journal Article
Open access
PDF file
DHDuc Minh Hoang
Duc Minh Hoang
THTran Thi Khanh Hoa
Tran Thi Khanh Hoa
CHCam Thi Thu Ha
Cam Thi Thu Ha
LHLe Van Hung
Le Van Hung
NTNguyen Van Thang
Nguyen Van Thang
GFGary Alan Flory
Gary Alan Flory
Clostridium perfringens is one of the most important zoonotic pathogens as it can cause food poisoning in humans and necrotic enteritis in both animals and humans. Meat, especially pork and chicken meat, is considered the main vehicle for the transmission of C. perfringens from animals to humans. The purpose of this study was to determine the prevalence, toxinotype, and antimicrobial resistance profile of C. perfringens isolated from pork and chicken meat sold in Vietnam. The isolation results showed that 15/50 (30%) of pork samples and 8/50 (16%) of chicken meat samples were contaminated with C. perfringens. The isolates exhibited their highest resistance rate to tetracycline (21/23; 91.30%) and clindamycin (10/23; 43.48%). On the contrary, their lowest resistance rates were observed in response to imipenem (2/23; 8.70%) and cefoxitin (1/23; 4.35%). In particular, 34.78% (8/23) of C. perfringens isolates were identified to be multidrug-resistant strains. The results of toxin genotyping indicated that all isolates were positive for the cpa gene and belonged to type A.

9 February 2023 in Pathogens
Verified
Journal Article
Open access
PDF file
TGTessa Carrau Garreta
Tessa Carrau Garreta
AMAlvydas Malakauskas
Alvydas Malakauskas
MMMarius Masiulis
Marius Masiulis
PBPaulius Bušauskas
Paulius Bušauskas
SJSigitas Japertas
Sigitas Japertas
GFGary Alan Flory
Gary Alan Flory
African swine fever (ASF) continues to spread and persist in the Eurasian wild boar population. The infection pressure resulting from infected carcasses in the environment can be a major contributor to disease persistence and spread. For this reason, it is crucial to find a safe and efficient method of carcass disposal under different circumstances. In the presented study, we investigated open-air composting of carcasses under winter conditions in northeastern Europe, i.e., Lithuania. We can demonstrate that the ASF virus (ASFV) is inactivated in both entire wild boar carcasses and pieces thereof in a time- and temperature-dependent manner. Composting piles reached up to 59.0 °C, and ASFV was shown to be inactivated. However, the ASFV genome was still present until the end of the 112-day sampling period. While further studies are needed to explore potential risk factors (and their mitigation), such as destruction of composting piles by scavengers or harsh weather conditions, composting seems to present a valid method to inactivate the ASFV in wild boar carcasses where rendering or other disposal methods are not feasible. In summary, composting provides a new tool in our toolbox of ASF control in wild boar and can be considered for carcass disposal.

16 May 2023 in Pathogens
Verified
Journal Article
Open access
PDF file
MHMark Hutchinson
Mark Hutchinson
DHDuc Minh Hoang
Duc Minh Hoang
GFGary Alan Flory
Gary Alan Flory
PNPham Hong Ngan
Pham Hong Ngan
HMHoang Minh
Hoang Minh
RFRenée Flory
Renée Flory
Identifying and ensuring the inactivation of the African Swine Fever virus in deadstock is a gap in the swine industry’s knowledge and response capabilities. The results of our study demonstrate that ASFv in deadstock was inactivated using static aerated composting as the carcass disposal method. Replicated compost piles with whole market hogs and two different carbon sources were constructed. In-situ bags containing ASFv-infected spleen tissue were placed alongside each of the carcasses and throughout the pile. The bags were extracted at days 0, 1, 3, 7, 14, 28, 56, and 144 for ASFv detection and isolation. Real-time PCR results showed that DNA of ASFv was detected in all samples tested on day 28. The virus concentration identified through virus isolation was found to be below the detection limit by day 3 in rice hulls and by day 7 in sawdust. Given the slope of the decay, near-zero concentration with 99.9% confidence occurred at 5.0 days in rice hulls and at 6.4 days in sawdust. Additionally, the result of virus isolation also showed that the virus in bone marrow samples collected at 28 days was inactivated.