Characterizing the gut microbiotas of wild birds across seasons will shed light on their annual life cycle. Due to sampling difficulties and the lack of detailed dietary information, studies on how the gut microbiota adapts to seasonal dietary changes of wild birds are scarce.

The Emei Shan Liocichla (Liocichla omeiensis) is an endemic bird species to southwestern China with a small geographic range. However, little was known about the genetic status of this threatened species. We applied restriction-site-associated DNA sequencing (RAD-Seq) for rapid mass identification of microsatellite markers of the Emei Shan Liocichla. A total of 11,564 microsatellite sequences were obtained, 600 random loci were designed for screening and 24 polymorphic microsatellite loci were selected for further validation. The average allele number, average observed heterozygosity and average expected heterozygosity were relatively low in our samples, which were 6.08, 0.6618 and 0.7048, respectively, indicating that the Emei Shan Liocichla might have lost some genetic diversity. Further analyses suggested that the populations distributed on two mountains (Daxiangling and Xiaoliangshan) showed a modest degree of genetic differentiation. These novel microsatellite markers provided valuable preliminary knowledge regarding the genetic status of the Emei Shan Liocichla and can be useful in further studies, as well as in the management and conservation of this species.

In this study, we report an unusual homing behavior of the Sichuan Partridge (Arborophila rufipectus) at the Laojunshan National Nature Reserve, Sichuan Province, China. Hen Sichuan Partridges led the chicks back to the nests where they hatched in the evening and roosted there over night. This behavior lasted 6.7 ± 4.3 nights (range = 1–15; n = 13) after the chicks hatched. At this stage, the hens became very vigilant to predators and human disturbance. If disturbed, they often abandoned the nests immediately and no longer returned thereafter. The ambient temperature at night during the early brooding period of Sichuan Partridge at our study site was ~ 12.4 °C. Our findings suggest that hen Sichuan Partridges may make trade-offs between nest predation risks versus the thermoregulatory needs of their young.

Habitat utilization is a critical determinant of animal survival and reproductive success. Clarifying species-specific habitat preferences provides essential insights into ecological requirements and forms the basis for sound conservation planning. The Temminck’s Tragopan (Tragopan temminckii), a medium-sized, sexually dimorphic pheasant endemic to montane forests of central and southern China, is classified as a nationally protected Class II species. Nevertheless, its fine-scale habitat selection during the breeding season remains inadequately documented. In 2024, we conducted a field investigation in the Laojunshan National Nature Reserve, Sichuan Province, to examine microhabitat use during this critical period. Our analysis revealed a significant preference for sites characterized by greater tree and bamboo height, higher canopy and bamboo cover, increased litter coverage, and taller shrub layers. In contrast, the species consistently avoided locations dominated by dense, tall herbaceous vegetation. Principal Component Analysis identified six principal components, collectively explaining 71.78% of the total environmental variance. The first component was primarily associated with bamboo structural attributes, the second with tree-layer structure, and the third with proximity to forest edges and streams. These findings indicate that effective conservation of this pheasant requires targeted forest management practices that preserve this specific suite of habitat characteristics, which are essential for ensuring reproductive success and long-term population viability.