To explore the characteristics of overland flow resistance under the condition of sparse vegetative stem coverage and improve the basic theoretical research of overland flow, the resistance characteristics of overland flow were systematically investigated under four slope gradients (S), seven flow discharges (Q), and six degrees of vegetation coverage (Cr). The results show that the Manning roughness coefficient (n) changes with the ratio of water depth to vegetation height (h/hv) while the Reynolds number (Re), Froude number (Fr), and slope (S) are closely related to vegetation coverage. Meanwhile, h/hv, Re, and Cr have strong positive correlations with n, while Fr and S have strong negative correlations with n. Through data regression analysis, a power function relationship between n and hydraulic parameters was observed and sensitivity analysis was performed. It was concluded that the relationship between n and h/hv, Re, Cr, Q, and S shows the same law; in particular, for sparse stem vegetation coverage, Cr is the dominant factor affecting overland flow resistance under zero slope condition, while Cr is no longer the first dominant factor affecting overland flow resistance under non-zero slope condition. In the relationship between n and Fr, Cr has the least effect on overland flow resistance. This indicates that when Manning roughness coefficient is correlated with different hydraulic parameters, the same vegetation coverage has different effects on overland flow resistance. Therefore, it is necessary to study overland flow resistance under the condition of sparse stalk vegetation coverage.