Luminescent carbon quantum dots (CQDs) prepared from aqueous beetroot extract were developed as unique fluorescent nanomaterials for in vivo live animal imaging applications. Blue (B) and green (G) emitting environmentally benign CQDs (particle size of 5 nm and 8 nm, respectively) exhibited bright fluorescence in aqueous medium and were found to be biocompatible, photostable and non-toxic in animal models. The in vivo imaging and toxicity evaluation of both CQDs were performed for the first time in the Caenorhabditis elegans (C. elegans) model, which revealed consistent fluorescence in the gut tissues of the worms without exerting any sign of toxic effects on the nematodes. The in vivo bio-distribution of G-CQDs given by tail vein injection in live BALB/c mice showed optical signals in the lower abdominal regions, mainly in the intestine, and cleared from the body through faeces. The tremendous potential shown by these eco-friendly CQDs in the C. elegans and mice models advocates new hopes for greener CQD nanomaterials as diagnostic tools in the biomedical field.

A mixture of extracts from two common vegetables, red pomegranate and turmeric, when photoexcited at 380 nm, produced almost pure white light emission (WLE) with Commission Internationale d’Eclairage (CIE) chromaticity index (0.35, 0.33) in acidic ethanol. It was also possible to obtain WLE in polyvinyl alcohol film (0.32, 0.25) and in gelatin gel (0.26, 0.33) using the same extract mixture. The colour temperature of the WLE was conveniently tunable by simply adjusting the concentrations of the component emitters. The primary emitting pigments responsible for contributing to WLE were polyphenols and anthocyanins from pomegranate and curcumin from turmeric. It was observed that a cascade of Forster resonance energy transfer involving polyphenolics, curcumin and anthocyanins played a crucial role in obtaining a CIE index close to pure white light. The optimized methods of extraction of the two primary emitting pigments from their corresponding plant sources are simple, cheap and fairly green.

Red emitting fluorescent carbon nanomaterials have drawn significant scientific interest in recent years due to their high quantum yield, water-dispersibility, photostability, biocompatibility, ease of surface functionalization, low cost and eco-friendliness. The red emissive characteristics of fluorescent carbon nanomaterials generally depend on the carbon source, reaction time, synthetic approach/methodology, surface functional groups, average size, and other reaction environments, which directly or indirectly help to achieve red emission. The importance of several factors to achieve red fluorescent carbon nanomaterials is highlighted in this review. Numerous plausible theories have been explained in detail to understand the origin of red fluorescence and tunable emission in these carbon-based nanostructures. The above advantages and fluorescence in the red region make them a potential candidate for multifunctional applications in various current fields. Therefore, this review focused on the recent advances in the synthesis approach, mechanism of fluorescence, and electronic and optical properties of red-emitting fluorescent carbon nanomaterials. This review also explains the several innovative applications of red-emitting fluorescent carbon nanomaterials such as biomedicine, light-emitting devices, sensing, photocatalysis, energy, anticounterfeiting, fluorescent silk, artificial photosynthesis, etc. It is hoped that by choosing appropriate methods, the present review can inspire and guide future research on the design of red emissive fluorescent carbon nanomaterials for potential advancements in multifunctional applications.

White light emission was produced upon 380 nm photoexcitation from a mixture of pomegranate extract and carbon nanoparticles made from the extract in aqueous acidic (pH 1.2) solution as well as in polyvinyl alcohol polymeric film with good Commission Internationale del’Eclairage (CIE) index values (0.28, 0.33) and (0.33, 0.29) respectively.