Quality assessment of a protein model is to predict the absolute or relative quality of a protein model using computational methods before the native structure is available. Single-model methods only need one model as input and can predict the absolute residue-specific quality of an individual model. Here, we have developed four novel single-model methods (Wang_deep_1, Wang_deep_2, Wang_deep_3, and Wang_SVM) based on stacked denoising autoencoders (SdAs) and support vector machines (SVMs). We evaluated these four methods along with six other methods participating in CASP11 at the global and local levels using Pearson’s correlation coefficients and ROC analysis. As for residue-specific quality assessment, our four methods achieved better performance than most of the six other CASP11 methods in distinguishing the reliably modeled residues from the unreliable measured by ROC analysis; and our SdA-based method Wang_deep_1 has achieved the highest accuracy, 0.77, compared to SVM-based methods and our ensemble of an SVM and SdAs. However, we found that Wang_deep_2 and Wang_deep_3, both based on an ensemble of multiple SdAs and an SVM, performed slightly better than Wang_deep_1 in terms of ROC analysis, indicating that integrating an SVM with deep networks works well in terms of certain measurements.

In the past decades, the electrocardiogram (ECG) has been investigated as a promising biometric by exploiting the subtle discrepancy of ECG signals between subjects. However, the heart rate (HR) for one subject may vary because of physical activities or strong emotions, leading to the problem of ECG signal variation. This variation will significantly decrease the performance of the identification task. Particularly for short-term ECG signal without many heartbeats, the hardly measured HR makes the identification task even more challenging. This study aims to propose a novel method suitable for short-term ECG signal identification. In particular, an improved HR-free resampling strategy is proposed to minimize the influence of HR variability during heartbeat processing. For feature extraction, the Principal Component Analysis Network (PCANet) is implemented to determine the potential difference between subjects. The proposed method is evaluated using a public ECG-ID database that contains various HR data for some subjects. Experimental results show that the proposed method is robust to HR change and can achieve high subject identification accuracy (94.4%) on ECG signals with only five heartbeats. Thus, the proposed method has the potential for application to systems that use short-term ECG signals for identification (e.g., wearable devices).

By combining convolutional neural networks (CNN) and long short term memory networks (LSTM) into the learning structure, this paper presents a supervised learning method called combining deep neural networks (CDNN) for protein secondary structure prediction. First, we use multiple convolutional neural networks with different number of layers and different size of filters to extract the protein secondary structure features. Second, we use bidirectional LSTM to extract features continually based on the raw features and the features which extracted by CNNs. Third, a fully connected dense layer is used to map the extracted features by LSTM to the different protein secondary structure classes. CDNN architecture is trained by RMSProp optimizer based on the cross entropy error between protein secondary structure labels and dense layer’s outputs. CDNN not only inherits the abstraction ability of CNN and sequence data process ability of LSTM, but also demonstrates the attractive classification ability for handling protein secondary structure data. The empirical validation on two protein secondary structure prediction datasets demonstrates the effectiveness of CDNN method.

RNA-binding proteins are important for the process of cell life activities. High-throughput technique experimental method to discover RNA-protein binding sites is time-consuming and expensive. Deep learning is an effective theory for predicting RNA-protein binding sites. Using weighted voting method to integrate multiple basic classifier models can improve model performance. Thus, in our study, we propose a weighted voting deep learning model (WVDL), which uses weighted voting method to combine convolutional neural network (CNN), long short term memory network (LSTM) and residual network (ResNet). First, the final forecast result of WVDL outperforms the basic classifier models and other ensemble strategies. Second, WVDL can extract more effective features by using weighted voting to find the best weighted combination. And, the CNN model also can draw the predicted motif pictures. Third, WVDL gets a competitive experiment result on public RBP-24 datasets comparing with other state-of-the-art methods. The source code of our proposed WVDL can be found in https://github.com/biomg/WVDL .

Computational prediction of the RBP bound sites using features learned from existing annotation knowledge is an effective method because high-throughput experiments are complex, expensive and time-consuming. Many methods have been proposed to predict RNA-protein binding sites. However, the partial information of RNA sequence is not fully used. In this study, we propose multiple convolutional neural networks (MCNN) method, which predicts RNA-protein binding sites by integrating multiple convolutional neural networks constructed by RNA sequence information extracted from windows with different lengths. First, MCNN trains multiple CNNs base on RNA sequences extracted by different window lengths. Second, MCNN can extract more binding patterns of RBPs by combining these trained multiple CNNs previously. Third, MCNN only uses RNA base sequence information for RNA-protein binding sites prediction, which extracts sequence binding features and predicts the result with same architecture. This avoids the information loss of feature extraction step. Our proposed MCNN demonstrates a competitive performance comparing with other methods on a large-scale dataset derived from CLIP-seq, which is an effective method for RNA-protein binding sites prediction. The source code of our proposed MCNN method can be found in https://github.com/biomg/MCNN.