The effort to implement more environmental-friendly fuels has been enhanced not only to reduce the greenhouse effect but also for public health issues. This paper studies the effects on pollutant emissions from a light-duty Euro 6 vehicle of four types of fuel: diesel (fossil origin, used as reference), biodiesel (renewable origin), Gas-to-Liquid (fossil origin) and farnesane (renewable origin). A double approach methodology is presented: stationary engine modes and driving cycles are studied. First, each fuel was tested in stationary modes in a vehicle test-bench and then tested in a realistic driving cycle with the same vehicle. This allows separating the transient effects of the driving cycle from stationary results. Stationary tests lead to engine emission maps and driving cycle tests have allowed weighting the importance of each stationary condition during a realistic route. Instantaneous and cumulative CO, THC (total hydrocarbon), NOx and PN (particle number) emissions on route were obtained.