Introduction: This study involved an analysis of the impact of mental fatigue on heart rate recovery (HRR), subjective measures of fatigue and intermittent running performance in handball players. Objective: This study was aimed at (1) examining the effects of an induced state of mental fatigue on the aerobic performance of handball players, as measured by the Yo-Yo IR1 test, and (2) exploring possible changes in heart rate regulation through HRR analysis. Methods: Twelve handball players (age: 17.50 ± 3.63 years; 5 ± 2.2 years of practice) undertook a Yo-Yo IR1 test on two occasions, separated by an interval of at least 72 hours. The Yo-Yo IR1 test was preceded by a 30-min treatment, consisting of the Stroop Color-Word Test, to induce mental fatigue. Participants in the control condition watched an emotionally neutral video. Results: Higher ratings of mental fatigue and mental effort following the Stroop Test were observed for the experimental group. No differences in motivation were observed between conditions. Moreover, the induction of mental fatigue impaired running performance and led to a higher RPE during the Yo-Yo IR1 test. Notwithstanding, no changes in HRR or blood lactate levels were observed across conditions. Conclusion: Altogether, these results suggest that mental fatigue impairs intermittent running performance, without affecting HRR values. Level of Evidence III; Case-Control study.

Antarctic climate is challenging, since the cold, wind and sensory monotony are stressful stimuli to individuals. Moreover, camp activities and heavy clothes may contribute to increase physiological strain. Thus, we aimed to characterise the physiological demand of a 24-day period in the Antarctic field and then to evaluate the effect of this expedition on the aerobic fitness in individuals with heterogeneous initial aerobic fitness (as determined by estimating maximum oxygen consumption – V̊O_2MAX). Before and after the 24-day period in Antarctica, 7 researchers and 2 mountaineers were subjected to incremental tests to estimate their V̊O_2MAX. Field effort was characterised by measuring heart rate (HR). During the field trips, their HR remained 33.4% of the recording time between 50–60% HR_MAX, 22.3% between 60–70% HR_MAX, and only 1.4% between 80 and 90% HR_MAX. The changes in estimated V̊O_2MAX during the expedition depended on the pre-expedition aerobic fitness. The post-expedition V̊O_2MAX increased by 5.9% and decreased by 14.3%in individuals with lower (researchers) and higher (mountaineers) initial V̊O_2MAX, respectively. We concluded that physical effort in the Antarctic field is characterised as predominantly of low- to moderate-intensity. This effort represented an effective training load for individuals with lower initial V̊O_2MAX, but not for those with higher V̊O_2MAX.

Antarctic camps pose psychophysiological challenges related to isolated, confined, and extreme (ICE) conditions, including meals composed of sealed food. ICE conditions can influence the microbiome and inflammatory responses. Seven expeditioners took part in a 7-week Antarctic summer camp (Nelson Island) and were evaluated at Pre-Camp (i.e., at the beginning of the ship travel), Camp-Initial (i.e., 4th and 5th day in camp), Camp-Middle (i.e., 19th–20th, and 33rd–34th days), Camp-Final (i.e., 45th–46th day), and at the Post-Camp (on the ship). At the Pre-Camp, Camp-Initial, and Camp-Final, we assessed microbiome and inflammatory markers. Catecholamines were accessed Pre- and Post-Camp. Heart rate variability (HRV), leptin, thyroid stimulating hormone (TSH), and thyroxine (T4) were accessed at all time points. Students’ t-tests or repeated-measures analysis of variance (one or two-way ANOVA) followed by Student-Newman-Keuls (post hoc) were used for parametric analysis. Kruskal-Wallis test was applied for non-parametric analysis. Microbiome analysis showed a predominance of Pseudomonadota (34.01%), Bacillota (29.82%), and Bacteroidota (18.54%), followed by Actinomycetota (5.85%), and Fusobacteria (5.74%). Staying in a long-term Antarctic camp resulted in microbiome fluctuations with a reduction in Pseudomonadota—a “microbial signature” of disease. However, the pro-inflammatory marker leptin and IL-8 tended to increase, and the angiogenic factor VEGF was reduced during camp. These results suggest that distinct Antarctic natural environments and behavioral factors modulate oral microbiome and inflammation.

This study systematically reviewed the literature reporting the changes in rats’ core body temperature (T_CORE) induced by either incremental- or constant-speed running to fatigue or exhaustion. In addition, multiple linear regression analyses were used to determine the factors contributing to the T_CORE values attained when exercise was interrupted. Four databases (EMBASE, PubMed, SPORTDiscus, and Web of Science) were searched in October 2021, and this search was updated in August 2022. Seventy-two studies (n = 1,538 rats) were included in the systematic review. These studies described heterogeneous experimental conditions; for example, the ambient temperature ranged from 5 to 40°C. The rats quit exercising with T_CORE values varying more than 8°C among studies, with the lowest and highest values corresponding to 34.9°C and 43.4°C, respectively. Multiple linear regression analyses indicated that the ambient temperature (p < 0.001), initial T_CORE (p < 0.001), distance traveled (p < 0.001; only incremental exercises), and running speed and duration (p < 0.001; only constant exercises) contributed significantly to explaining the variance in the T_CORE at the end of the exercise. In conclusion, rats subjected to treadmill running exhibit heterogeneous T_CORE when fatigued or exhausted. Moreover, it is not possible to determine a narrow range of T_CORE associated with exercise cessation in hyperthermic rats. Ambient temperature, initial T_CORE, and physical performance-related variables are the best predictors of T_CORE at fatigue or exhaustion. From a broader perspective, this systematic review provides relevant information for selecting appropriate methods in future studies designed to investigate exercise thermoregulation in rats.