Salvia miltiorrhizaBunge, which contains tanshinones and phenolic acids as major classes of bioactive components, is one of the most widely used herbs in traditional Chinese medicine. Production of tanshinones and phenolic acids is enhanced by methyl jasmonate (MeJA). Transcription factor MYC2 is the switch of jasmontes signaling in plants. Here, we focused on two novel JA-inducible genes inS. miltiorrhiza, designated asSmMYC2aandSmMYC2b, which were localized in the nucleus.SmMYC2a andSmMYC2b were also discovered to interact withSmJAZ1 andSmJAZ2, implying that the two MYC2s might function as direct targets of JAZ proteins. Ectopic RNA interference (RNAi)-mediated knockdown experiments suggested thatSmMYC2a/b affected multiple genes in tanshinone and phenolic acid biosynthetic pathway. Besides, the accumulation of tanshinones and phenolic acids was impaired by the loss of function inSmMYC2a/b. Meanwhile,SmMYC2a could bind with an E-box motif withinSmHCT6andSmCYP98A14promoters, whileSmMYC2b bound with an E-box motif withinSmCYP98A14promoter, through which the regulation of phenolic acid biosynthetic pathway might achieve. Together, these results suggest thatSmMYC2a andSmMYC2b are JAZ-interacting transcription factors that positively regulate the biosynthesis of tanshinones and Sal B with similar but irreplaceable effects.