Ideal bone tissue engineering scaffolds composed of extracellular matrix (ECM) require excellent osteoconductive ability to imitate the bone environment. We developed a mineralised tissue-derived ECM-modified true bone ceramic (TBC) scaffold for the delivery of aspartic acid-modified bone morphogenic protein-2 (BMP-2) peptide (P28) and assessed its osteogenic capacity. Decellularized ECM from porcine small intestinal submucosa (SIS) was coated onto the surface of TBC, followed by mineralisation modification (mSIS/TBC). P28 was subsequently immobilised onto the scaffolds in the absence of a crosslinker. The alkaline phosphatase activity and other osteogenic differentiation marker results showed that osteogenesis of the P28/mSIS/TBC scaffolds was significantly greater than that of the TBC and mSIS/TBC groups. In addition, to examine the osteoconductive capability of this system in vivo, we established a rat calvarial bone defect model and evaluated the new bone area and new blood vessel density. Histological observation showed that P28/mSIS/TBC exhibited favourable bone regeneration efficacy. This study proposes the use of mSIS/TBC loaded with P28 as a promising osteogenic scaffold for bone tissue engineering applications.

The effects of finishing rolling temperature on the microstructure and mechanical properties of a direct quenched and partitioned (DQ&P) steel were investigated by a thermal simulation machine, a field emission scanning electron microscope (FE-SEM), electron backscattering diffraction (EBSD), and a transmission electron microscope (TEM). The results show that the original austenite grain size was refined by 31% as the finishing rolling temperature decreased from 920 °C to 840 °C, leading to the formation of the finest martensite lath at 840 °C. At the same time, the lower finishing rolling temperature resulted in a higher dislocation density, and consequently improved the stability of the retained austenite. Moreover, compared to the conventional Q&P process, the comprehensive mechanical properties of a steel with similar chemical composition can be enhanced by DQ&P processing. With the decrease of finishing rolling temperature from 920 °C to 840 °C, the strength and total elongation increases. The yield strength, tensile strength, and total elongation reach the maximum values of 1121 MPa, 1134 MPa, and 11.7%, respectively, at 840 °C.

The microstructure and wear performance of a low-carbon steel treated by austempering below and above martensite start temperature (Ms) were investigated. The results show that the bainite, fresh martensite (FM) and retained austenite (RA) were observed in samples austempered above Ms. Except for the three above phases, the athermal martensite (AM) was also observed in samples austempered below Ms. The bainite transformation was accelerated and finer bainite was obtained due to the AM formation in samples austempered below Ms. In addition, the strength and hardness were improved with the decrease of the isothermal temperature and time, whereas the total elongation decreased with the increasing isothermal time and the decreasing isothermal temperature. Moreover, the materials austempered below Ms exhibited better wear performance than the ones treated above Ms, which is attributed to the improved impact toughness by the finer bainite and the enhanced hardness by AM. The best wear resistance was obtained in the samples austempered at 300 °C below Ms for 200 s, due to the highest hardness and considerable impact toughness.

Previous studies on the austenite grain growth were mostly based on a fixed temperature, and the relationship between the austenite grain and austenitizing parameters was fitted according to the results. However, there is a lack of quantitative research on the austenite grain growth during the heating process. In the present work, based on the diffusion principle of the controlled Ti microalloying element, the diffusion process of carbonitrides containing Ti during the heating process was analyzed. Combined with the precipitation model and the austenite growth model, the prediction model of austenite grain growth of Ti microalloyed steel during different heat treatment processes was established. The austenite grain size versus the temperature at four different heating rates of 0.5, 1, 10, 100 °C/s was calculated. The grain growth behavior of austenite during the heating process of Ti microalloyed steel was studied by optical microscope, scanning electron microscope and transmission electron microscope. The experimental data of the austenite grain size was in good agreement with the calculation by the proposed model, which provides a new idea for the prediction of austenite grain size in non-equilibrium state during the heating process. In addition, for Ti-containing microalloyed steels, the austenite grain size increased with the increasing heating temperature, while it changed little by further prolonging isothermal time after certain heating time, which was related to the equilibrium degree of the precipitation and the dissolution of Ti element. The austenite grain coarsening temperature of the tested Ti microalloyed steel was estimated within 1100~1200 °C.