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Prof. HaengHoon Kim
Department of Agro-bio-science, Sunchon National University, Suncheon 57922, Republic of Korea

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Research Keywords & Expertise

0 Cryopreservation
0 Neuroscience
0 Life Science, Microbiology, Bioinformatics, Biotechnology, Molecular Biology
0 Biodiversity & Conservation
0 in vitro culture, micropropagation

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Journal article
Published: 05 January 2021 in Horticulturae
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Quantitative HPLC analysis was performed on six different species of Lycoris herbs to investigate variation in phytochemical content, especially galantamine and phenylpropanoid-derived compounds. The contents of these compounds differed widely among the Lycoris species, with L. radiata and L. chinensis containing the lowest and highest galantamine contents, respectively. Specifically, the galantamine content of L. radiata was 62.5% higher than that of L. chinensis. Following L. radiata, L. sanguinea contained the next highest galantamine content, which was 59.1% higher than that of L. chinensis. Furthermore, a total of 12 phenylpropanoid-derived compounds were found in the different Lycoris species, where L. sanguinea, L. squamigera, and L. uydoensis had the largest accumulation of these compounds. The total phenylpropanoid content of L. sanguinea was the highest, while that of L. radiata was the lowest. Seven of the phenylpropanoid-derived compounds, rutin, quercetin, catechin, epicatechin gallate, chlorogenic acid, benzoic acid, and kaempferol, were dominant. L. sanguinea, L. uydoensis, and L. squamigera showed amounts of these seven compounds that were 5–6 times greater than those of the other species in the study. To the best of our knowledge, our results provide the most detailed phytochemical information on these species to date, which is valuable for future applications using these medicinal plants.

ACS Style

Hyeon Ji Yeo; Ye Jin Kim; Bao Van Nguyen; Ye Eun Park; Chang Ha Park; Haeng Hoon Kim; Jae Kwang Kim; Sang Un Park. Comparison of Secondary Metabolite Contents and Metabolic Profiles of Six Lycoris Species. Horticulturae 2021, 7, 5 .

AMA Style

Hyeon Ji Yeo, Ye Jin Kim, Bao Van Nguyen, Ye Eun Park, Chang Ha Park, Haeng Hoon Kim, Jae Kwang Kim, Sang Un Park. Comparison of Secondary Metabolite Contents and Metabolic Profiles of Six Lycoris Species. Horticulturae. 2021; 7 (1):5.

Chicago/Turabian Style

Hyeon Ji Yeo; Ye Jin Kim; Bao Van Nguyen; Ye Eun Park; Chang Ha Park; Haeng Hoon Kim; Jae Kwang Kim; Sang Un Park. 2021. "Comparison of Secondary Metabolite Contents and Metabolic Profiles of Six Lycoris Species." Horticulturae 7, no. 1: 5.

Journal article
Published: 20 November 2020 in Plants
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Plants are continuously exposed to abiotic and biotic factors that lead to wounding stress. Different plants exhibit diverse defense mechanisms through which various important metabolites are synthesized. Humans can exploit these mechanisms to improve the efficacy of existing drugs and to develop new ones. Most previous studies have focused on the effects of wounding stress on the different plant parts, such as leaves, stems, and roots. To date, however, no study has investigated the accumulation of primary and galantamine content following the exposure of a callus to wounding stress. Therefore, in the present study, we exposed Lycoris radiata calli to wounding stress and assessed the expression levels of several genes involved in metabolic pathways at various time points (0, 3, 6, 12, 24, 48, 72, and 96 h of exposure). Furthermore, we quantify the primary and galantamine content using gas chromatography–time-of-flight mass spectrometry and the high-performance liquid chromatography qRT-PCR analysis of eight galantamine pathway genes (LrPAL-2, LrPAL-3, LrC4H-2, LrC3H, LrTYDC2, LrN4OMT, LrNNR, and LrCYP96T) revealed that seven genes, except LrN4OMT, were significantly expressed following exposure to wounding stress. Galantamine contents of calli after 3, 6, 12, 24, 48, 72, and 96 h of exposure were respectively 2.5, 2.5, 3.5, 3.5, 5.0, 5.0, and 8.5 times higher than that after 0 h of exposure. Furthermore, a total of 48 hydrophilic metabolites were detected in the 0 h exposed callus and 96 h exposed callus using GC-TOFMS. In particular, a strong positive correlation between galantamine and initial precursors, such as phenylalanine and tyrosine, was observed.

ACS Style

Chang Ha Park; Ramaraj Sathasivam; Bao Van Nguyen; Seung-A Baek; Hyeon Ji Yeo; Ye Eun Park; Haeng Hoon Kim; Jae Kwang Kim; Sang Un Park. Metabolic Profiling of Primary Metabolites and Galantamine Biosynthesis in Wounded Lycoris radiata Callus. Plants 2020, 9, 1616 .

AMA Style

Chang Ha Park, Ramaraj Sathasivam, Bao Van Nguyen, Seung-A Baek, Hyeon Ji Yeo, Ye Eun Park, Haeng Hoon Kim, Jae Kwang Kim, Sang Un Park. Metabolic Profiling of Primary Metabolites and Galantamine Biosynthesis in Wounded Lycoris radiata Callus. Plants. 2020; 9 (11):1616.

Chicago/Turabian Style

Chang Ha Park; Ramaraj Sathasivam; Bao Van Nguyen; Seung-A Baek; Hyeon Ji Yeo; Ye Eun Park; Haeng Hoon Kim; Jae Kwang Kim; Sang Un Park. 2020. "Metabolic Profiling of Primary Metabolites and Galantamine Biosynthesis in Wounded Lycoris radiata Callus." Plants 9, no. 11: 1616.

Journal article
Published: 08 December 2016 in Cryo letters
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ACS Style

E Han; Elena Popova; G Cho; S Park; S Lee; H W Pritchard; H H Kim. POST-HARVEST EMBRYO DEVELOPMENT IN GINSENG SEEDS INCREASES DESICCATION SENSITIVITY AND NARROWS THE HYDRATION WINDOW FOR CRYOPRESERVATION. Cryo letters 2016, 37, 284 -294.

AMA Style

E Han, Elena Popova, G Cho, S Park, S Lee, H W Pritchard, H H Kim. POST-HARVEST EMBRYO DEVELOPMENT IN GINSENG SEEDS INCREASES DESICCATION SENSITIVITY AND NARROWS THE HYDRATION WINDOW FOR CRYOPRESERVATION. Cryo letters. 2016; 37 (4):284-294.

Chicago/Turabian Style

E Han; Elena Popova; G Cho; S Park; S Lee; H W Pritchard; H H Kim. 2016. "POST-HARVEST EMBRYO DEVELOPMENT IN GINSENG SEEDS INCREASES DESICCATION SENSITIVITY AND NARROWS THE HYDRATION WINDOW FOR CRYOPRESERVATION." Cryo letters 37, no. 4: 284-294.

Book chapter
Published: 01 January 2015 in Advances in Plant Breeding Strategies: Breeding, Biotechnology and Molecular Tools
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Plant biodiversity is crucial for sustaining human life on our planet. More than 50,000 species are used globally for food, feed, fiber, medicine and horticulture. A wide range of plant-based biotechnological systems such as isolated root cultures, embryogenic cell and tissue cultures and cell suspensions are used in breeding programs, forestry and the production of pharmaceuticals. Cryopreservation is an essential tool for conservation and long-term maintenance of diverse germplasms with minimal requirements for cost and labor and a low risk of loss of preserved samples. However, large-scale use of cryogenic storage to back-up plant genetic collections is hampered by unavailability of effective methodology and genotype-specific responses of diverse specimens to cryoprotective treatments. Newly developed techniques such as droplet-vitrification are more effective and user-friendlier than classical methods of cryopreservation. Cryopreservation has been successfully employed for preserving several different types of plant materials. In this chapter we review various approaches to develop and improve cryopreservation protocols for diverse plant species. Applications of modern cryopreservation methods in biotechnology-based industry as well as breeding programs are also discussed.

ACS Style

Elena Popova; Mukund Shukla; Haeng Hoon Kim; Praveen K. Saxena. Plant Cryopreservation for Biotechnology and Breeding. Advances in Plant Breeding Strategies: Breeding, Biotechnology and Molecular Tools 2015, 63 -93.

AMA Style

Elena Popova, Mukund Shukla, Haeng Hoon Kim, Praveen K. Saxena. Plant Cryopreservation for Biotechnology and Breeding. Advances in Plant Breeding Strategies: Breeding, Biotechnology and Molecular Tools. 2015; ():63-93.

Chicago/Turabian Style

Elena Popova; Mukund Shukla; Haeng Hoon Kim; Praveen K. Saxena. 2015. "Plant Cryopreservation for Biotechnology and Breeding." Advances in Plant Breeding Strategies: Breeding, Biotechnology and Molecular Tools , no. : 63-93.

Journal article
Published: 07 September 2014 in Applied Biochemistry and Biotechnology
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Differential expression patterns of flavonoid biosynthetic pathway genes in the hairy roots of tartary buckwheat cultivars “Hokkai T8” and “Hokkai T10” were studied over a time course of the light–dark cycle. The Agrobacterium rhizogenes-mediated transformation system was applied for inducing hairy roots. Further, a total of six phenolic compounds and two anthocyanins were analyzed in the hairy roots which were exposed to both light and dark conditions, and their amounts were estimated by HPLC. The gene expression levels peaked on day 5 of culture during the time course of both dark and light conditions. Notably, FtPAL, Ft4CL, FtC4H, FtCHI, FtF3H, FtF3’H-1, and FtFLS-1 were more highly expressed in Hokkai T10 than in Hokkai T8 under dark conditions, among which FtPAL and FtCHI were found to be significantly upregulated, except on day 20 of culture. Significantly higher levels of phenolic compound, rutin, along with two anthocyanins were detected in the hairy roots of Hokkai T10 under both conditions. Furthermore, among all the phenolic compounds detected, the amount of rutin in Hokkai T10 hairy roots was found to be ∼5-fold (59,01 mg/g dry weight) higher than that in the control (12.45 mg/g dry weight) at the respective time periods under light and dark conditions.

ACS Style

Aye Aye Thwe; Yeji Kim; Xiaohua Li; Yeon Bok Kim; Nam-Il Park; Haeng Hoon Kim; Sun-Ju Kim; Sang Un Park. Accumulation of Phenylpropanoids and Correlated Gene Expression in Hairy Roots of Tartary Buckwheat under Light and Dark Conditions. Applied Biochemistry and Biotechnology 2014, 174, 2537 -2547.

AMA Style

Aye Aye Thwe, Yeji Kim, Xiaohua Li, Yeon Bok Kim, Nam-Il Park, Haeng Hoon Kim, Sun-Ju Kim, Sang Un Park. Accumulation of Phenylpropanoids and Correlated Gene Expression in Hairy Roots of Tartary Buckwheat under Light and Dark Conditions. Applied Biochemistry and Biotechnology. 2014; 174 (7):2537-2547.

Chicago/Turabian Style

Aye Aye Thwe; Yeji Kim; Xiaohua Li; Yeon Bok Kim; Nam-Il Park; Haeng Hoon Kim; Sun-Ju Kim; Sang Un Park. 2014. "Accumulation of Phenylpropanoids and Correlated Gene Expression in Hairy Roots of Tartary Buckwheat under Light and Dark Conditions." Applied Biochemistry and Biotechnology 174, no. 7: 2537-2547.

Journal article
Published: 22 July 2013 in Molecules
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In this study, we investigated the expression of seven MYB transcription factors (a total of 17 genes that included Dof1.1, IQD1-1, MYB28, MYB29, MYB34, MYB51, and MYB122 and their isoforms) involved in aliphatic and indolic glucosinolate (GSL) biosynthesis and analyzed the aliphatic and indolic GSL content in different organs of Chinese cabbage (Brassica rapassp. Pekinensis). MYB28 and MYB29 expression in the stem was dramatically different when compared with the levels in the other organs. MYB34, MYB122, MYB51, Dof1.1, and IQD1-1 showed very low transcript levels among different organs. HPLC analysis showed that the glucosinolates (GSLs) consisted of five aliphatic GSLs (progoitrin, sinigrin, glucoalyssin, gluconapin, and glucobrassicanapin) and four indolic GSLs (4-hydroxyglucobrassicin, glucobrassicin, 4-methoxygluco-brassicin, and neoglucobrassicin). Aliphatic GSLs exhibited 63.3% of the total GSLs content, followed by aromatic GSL (19.0%), indolic GSLs (10%), and unknown GSLs (7.7%) in different organs of Chinese cabbage. The total GSL content of different parts (ranked in descending order) was as follows: seed > flower > young leaves > stem > root > old leaves. The relationship between GSLs accumulation and expression of GSLs biosynthesis MYB TFs genes in different organs may be helpful to understand the mechanism of MYB TFs regulating GSL biosynthesis in Chinese cabbage.

ACS Style

Yeon Bok Kim; Xiaohua Li; Sun-Ju Kim; Haeng Hoon Kim; Jeongyeo Lee; Hyeran Kim; Sang Un Park. MYB Transcription Factors Regulate Glucosinolate Biosynthesis in Different Organs of Chinese Cabbage (Brassica rapa ssp. pekinensis). Molecules 2013, 18, 8682 -8695.

AMA Style

Yeon Bok Kim, Xiaohua Li, Sun-Ju Kim, Haeng Hoon Kim, Jeongyeo Lee, Hyeran Kim, Sang Un Park. MYB Transcription Factors Regulate Glucosinolate Biosynthesis in Different Organs of Chinese Cabbage (Brassica rapa ssp. pekinensis). Molecules. 2013; 18 (7):8682-8695.

Chicago/Turabian Style

Yeon Bok Kim; Xiaohua Li; Sun-Ju Kim; Haeng Hoon Kim; Jeongyeo Lee; Hyeran Kim; Sang Un Park. 2013. "MYB Transcription Factors Regulate Glucosinolate Biosynthesis in Different Organs of Chinese Cabbage (Brassica rapa ssp. pekinensis)." Molecules 18, no. 7: 8682-8695.

Journal article
Published: 01 January 2012 in The Scientific World Journal
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Shoot organogenesis and plant regeneration inSinningia speciosawere improved using ethylene inhibitors. The leaf explants were cultured on initial shoot regeneration media (MS media with BAP at 2 mg/L + NAA at 0.1 mg/L) supplemented with different concentrations of aminoethoxyvinylglycine (AVG), cobalt chloride (CoCl2), and silver thiosulphate (STS). The addition of AVG, CoCl2, and STS significantly improved the regeneration frequency giving higher shoots per explant and longer shoot length. The highest shoot growth was found when STS at 5 mg/L was incorporated with generation medium, performing highest regeneration frequency with highest number of shoots. This treatment (STS at 5 mg/L) produced 40% more shoots per explant compared to control followed by STS at 10 mg/L with increasing 37% more shoots compared to control. In the cases of AVG and CoCl2the highest shoot number per explant was found at 1 mg/L. Treated with AVG and CoCl2at 1 mg/L increased shoot number by 16 and 12%, respectively, compared to control. Ethylene inhibitors could be used as a possible micropropagation and plant transformation protocol inS. speciosafor plant regenerations.

ACS Style

Soo Cheon Chae; Haeng Hoon Kim; Sang Un Park. Ethylene Inhibitors Enhance Shoot Organogenesis of Gloxinia (Sinningia speciosa). The Scientific World Journal 2012, 2012, 1 -4.

AMA Style

Soo Cheon Chae, Haeng Hoon Kim, Sang Un Park. Ethylene Inhibitors Enhance Shoot Organogenesis of Gloxinia (Sinningia speciosa). The Scientific World Journal. 2012; 2012 ():1-4.

Chicago/Turabian Style

Soo Cheon Chae; Haeng Hoon Kim; Sang Un Park. 2012. "Ethylene Inhibitors Enhance Shoot Organogenesis of Gloxinia (Sinningia speciosa)." The Scientific World Journal 2012, no. : 1-4.