The common strain black carp (Cyprinus carpio var. baisenensis) is a culturally important carp strain that is raised and cultured in Guangxi Province, China. Its color reflects the interactions between the Burau people and their surrounding environment. The population of the common carp black strain was isolated and cultured in a rice-fish integration system. To explore the genetic diversity and protection of germplasm resources, we analyzed mitochondrial DNA (mtDNA) sequences, specifically the displacement loop (D-loop) and cytochrome b (Cytb), using single-nucleotide polymorphisms (SNP). We compared these sequences with those from four other local common carp populations. The study included a total of 136 adult common carps from five strain populations: the common black carp strain (HJ = 31), Jian (F = 30), Heilongjiang (H = 10), Songpu (S = 31), and Saijiang (SJ = 34). The results of the Cytb and D-loop analyses showed that the Heilongjiang carp (H) and Saijiang (SJ) populations had the highest levels of haplotype diversity (0.867 ± 0.034785) and nucleotide diversity (π = 0.0063 ± 0.000137 and 0.0093 ± 0.000411), respectively. On the other hand, the Common carp black strain population (HJ) exhibited the lowest haplotype diversity in both Cytb and D-loop, with haplotype 2 being the most commonly observed among the populations. Private haplotypes dominated the five common carp populations, which were significantly different at P<0.001. Furthermore, analyzing the coefficient of genetic differentiation (Fst), the highest genetic difference was observed between Saijiang (SJ) and Heilongjiang (H) (Fst = 0.963), whereas the lowest was observed between Songpu (S) and the Common carp black strain population (HJ) (Fst = 0.019) for the Cytb gene sequences. For the D-loop, the Common carp black strain population (HJ) and Songpu (S) (Fst = 0.7) had the highest values, and Heilongjiang (H) and Common black carp strain (HJ) had an Fst of 0.125. Additionally, the AMOVA analysis revealed a higher level of variance for the Cytb and D-loop genes, indicating lower genetic diversity within the local carp community. On the other hand, the phylogenetic tree analysis showed that the five carp populations were closely related and formed a distinct cluster. The distinct cluster of populations suggests a common ancestor or recent gene flow, possibly due to geographic proximity or migration history, and unique genetic characteristics, possibly due to adaptations or selective pressures. The results of this study provide valuable insights into the genetic diversity of the common strain black carp, which can have implications for conservation, breeding programs, evolutionary studies, and fisheries management.

To gain a deeper understanding of the genetic factors influencing the growth and development of Eriocheir sinensis, a well-known species of hairy crab found in Yangcheng Lake, this study focused on the de novo genome and full-length transcriptome information of the selected subjects. Specifically, Yangcheng Lake hairy crabs were chosen as the experimental samples. Initially, a genome analysis was performed, resulting in the identification of gene fragments with a combined length of 1266,092,319 bp. Subsequently, a transcriptome analysis was conducted on a mixture of tissues from four different sites, namely muscle, brain, eye, and heart, to further investigate the genetic characteristics at the transcriptome level. The Pacific Biosciences (Pacio) single-molecule real-time sequencing system generated a total of 36.93 G sub-fragments and 175,90041 effective inserts. This research contributes to the indirect comprehension of genetic variations underlying individual traits. Furthermore, a comparison of the obtained data with relevant literature emphasizes the advantages of this study and establishes a basis for further investigations on the Chinese mitten crab.

ABTRACT The common strain black carp (Cyprinus carpio var. baisenensis), known for its black skin is cultured in the integrated rice-agriculture system and non-escape property under torrential floods. The total mitogenome length of Cyprinus carpio var. baisenensis obtained in this study was 16,478bp, consisting of 13 protein-coding genes, 22 tRNA genes, 2rRNA genes (large and small), a light strand origin of replication, and one major non-coding region. By providing the complete mitochondrial genomes of Cyprinus carpio var. baisenensis, we will further understand the phylogenetic relationships within genus.

Temperature is a critical abiotic factor regulating the physiology, growth, and reproduction of ectothermic aquatic animals. In China, the rapid expansion of the red swamp crayfish Procambarus clarkii) industry faces significant challenges due to seasonal temperature fluctuations (optimal growth at 20–25 °C and reproduction favored at 18–22 °C). This review focuses specifically on TRP channels, particularly TRPA1 as a key thermosensor in crayfish, and on downstream signaling pathways involving heat shock proteins (HSPs) and antioxidant defenses. We further link these biological mechanisms to aquaculture applications by evaluating best management practices for mitigating thermal stress, including integrated rice–crayfish co-culture, recirculating aquaculture systems (RAS), molecular marker-assisted breeding for thermal tolerance, and nutritional modulation (e.g., probiotics and immunostimulants). By maintaining thermal stability within the optimal range and directly enhancing physiological resilience through genetic and nutritional interventions, these practices provide a foundation for more sustainable and climate-resilient crayfish aquaculture.