Ladakhi cattle is native population of Leh and Ladakh region and constantly exposed to hypobaric hypoxia over many generations. In present study, transcriptome signatures of cattle from Ladakh region (~5500 m) and Sahiwal cattle from tropical regions were evaluated using Agilent 44 K microarray chip. The top up-regulated genes in Ladakhi cows were INHBC, ITPRI, HECA, ABI3, GPR171, and HIF-1α involved in hypoxia and stress response. In Sahiwal cows, the top up-regulated genes eEF1A1, GRO1, CXCL2, DEFB3 and BOLA-DQA3 were associated with immune function and inflammatory response indicating their strong immune potential to combat the pathogens prevalent in the tropical conditions. The molecular pathways highly impacted were MAPK signaling, ETC, apoptosis, TLR signaling and NF- kB signaling pathway indicating signatures of adaptive evolution of these two cattle types in response to diverse environments. Further, qPCR analysis revealed increased expression of DEGs such as HIF-1, EPAS-1, VEGFA, NOS2, and GLUT-1/SLC2A1 in cattle types from high altitude suggesting their pivotal role in association with high altitude adaptation. Based on data generated, native cattle of Ladakh region was found to be genetically distinct from native cattle adapted to the tropical region of India.

Identification of appropriate breeds of broilers and development of new feed additives is required for the development of poultry industry at high altitude. Therefore, this experiment was conducted first to identify the suitable broiler strain for this region. One week old chicks (150) from three broiler strains, i.e. Vencobb, RIR cross-bred, and Hubbard were randomly selected and divided equally into three groups. All the chicks were provided the same basal diet. The body weight gain and feed: gain responses were significantly (P < 0.05) improved in RIR cross-bred. Mortality was also observed lower in RIR cross-bred. Thereafter, the second trial was conducted in RIR cross-bred to evaluate the effect of probiotic supplementation (T1@ 9 gm/kg feed, T2@ 18 gm/kg feed) on their performance and mortality. No significant differences (P > 0.05) were observed in weight gain, feed intake, feed: gain, and water intake among the three groups, however, mortality from ascites and coccidiosis was reduced in probiotic treated groups. Hence, our results suggest that RIR cross-bred is suitable for rearing in high altitude regions and probiotic supplementation has no beneficial effects on production performance of broilers at high altitude. However, probiotic supplementation indicated lesser loss due to mortality of birds.

A total of seventy irrigation water samples were collected from Leh, Ladakh, India, to determine their hydrochemistry profile and water quality for irrigation purposes. Water quality indices such as total hardness (TH), residual sodium carbonate (RSC), potential salinity (PS), permeability index (PI), Kelly's ratio (KR), sodium absorption ratio (SAR), corrosivity ratio (CR), and chloroalkaline indices (CAI) were measured. The Piper diagram, Durov's diagram, and United States Salinity Laboratory (USSL) diagram were drawn to determine the water types, ion exchange processes, and rock–soil–water interaction. The findings indicated that the hydrochemical characteristics of irrigation water are naturally alkaline. Furthermore, water quality parameters revealed that the study area’s surface water is suitable for irrigation purposes, despite relatively high TH and MH levels. The level of CR indicated that water should not be transported using metal pipes. The CAI and Durov's plot indicated direct ionic exchange processes controlling water chemistry. Additionally, this irrigation water is mainly dominated by the Ca²⁺–Cl⁻–SO₄²⁻, Na⁺–K⁺–CaCO₃ + HCO₃⁻, and Na⁺–K⁺–Cl⁻ type, which indicates silicate rock weathering along with some anthropogenic input. The USSL salinity diagram indicated a medium salinity and low Na hazard. Hence, it is concluded that the rock weathering cycle is the natural source of all the cations and anions, and these ions pass directly from water to rock and vis-à-vis, along with some input of anthropogenic activity.

Extremes of climate and hypobaric hypoxia cause poor growth performance in broiler chickens at high altitude. The present study examined the potential ofHippophae rhamnoidesextract as phytogenic feed additive for broilers reared at 3500 m above mean sea level (MSL). Higher content of phytomolecules were recorded during characterization of the extract. Immunomodulatory activity of extract was observed in chicken lymphocytes throughin-vitrostudies. Thereafter, forin vivostudy, 105 day old Rhode Island Red (RIR) Cross-bred chicks were randomly distributed in to control and treatments T1, T2, T3, T4, T5, and T6 which were supplemented withH. rhamnoidesaqueous extract along with basal diet, at level of 100, 150, 200, 300, 400, and 800 mg/kg body weight of chicken, respectively. Among the experimental groups, birds in the T3 group represent the highest body weight. Furthermore, treatment group birds had shown better physio-biochemical indices as compared to control group birds. Interestingly, lower mortality rate due to ascites and coccidiosis was recorded in treatment groups and therefore, higher net return was observed. Hence, present investigation demonstrated the beneficial effect ofH. rhamnoidesextract (@200 mg/kg) at high altitude and therefore, may be used in formulation of feed additive for poultry ration.

Monitoring water quality of surface water resources is the key concern in determining the potable water quality in high-altitude region. Therefore, there is a need to evaluate different parameters affecting water quality of river and identify the most important variables and factors significantly affecting water quality. In the present study, multivariate statistical methods including cluster analysis and principal component analysis/factor analysis were applied to analyze the Indus River water quality in the Trans-Himalayan region of India. For this total 25 no. of physicochemical parameters were analyzed in water samples taken from seven different monitoring sites in summer and winter season. All the physical, microbial, chemical, and mineral parameters were analyzed by using the standard methods of American Public Health Association, whereas minerals were determined with the inductively coupled plasma optical emission of spectroscopy method. Thereafter, experimental two-season (28 samples × 25 parameters) matrices of both the seasons were run through the multivariate statistical data analysis. The varifactors obtained from the FA of both the seasons and results indicate that the parameters responsible for water quality variations are mainly related to discharge and temperature (natural), organic pollution (point source: domestic sanitary waste), and nutrients (non-point sources: agriculture) in the summer season. However, in the winter seasons, results showed that the river water was less affected by anthropogenic activities and natural weathering process. Therefore, it is concluded that quality of Indus River water is affected by agricultural, domestic, and hydrogeochemical sources in the summer season. These findings corroborate suitability of multivariate statistical techniques in the elucidation of various parameters for water quality monitoring and determination of different contamination sources.