It is important to improve the accuracy of models estimating aboveground biomass (AGB) in large areas with complex geography and high forest heterogeneity. In this study, k-nearest neighbors (k-NN), gradient boosting machine (GBM), random forest (RF), quantile random forest (QRF), regularized random forest (RRF), and Bayesian regularization neural network (BRNN) machine learning algorithms were constructed to estimate the AGB of four forest types based on environmental factors and the variables selected by the Boruta algorithm in Yunnan Province and using integrated Landsat 8 OLI and Sentinel 2A images. The results showed that (1) DEM was the most important variable for estimating the AGB of coniferous forests, evergreen broadleaved forests, deciduous broadleaved forests, and mixed forests; while the vegetation index was the most important variable for estimating deciduous broadleaved forests, the climatic factors had a higher variable importance for estimating coniferous and mixed forests, and texture features and vegetation index had a higher variable importance for estimating evergreen broadleaved forests. (2) In terms of specific model performance for the four forest types, RRF was the best model both in estimating the AGB of coniferous forests and mixed forests; the R² and RMSE for coniferous forests were 0.63 and 43.23 Mg ha⁻¹, respectively, and the R² and RMSE for mixed forests were 0.56 and 47.79 Mg ha⁻¹, respectively. BRNN performed the best in estimating the AGB of evergreen broadleaved forests; the R² was 0.53 and the RMSE was 68.16 Mg ha⁻¹. QRF was the best in estimating the AGB of deciduous broadleaved forests, with R² of 0.43 and RMSE of 45.09 Mg ha⁻¹. (3) RRF was the best model for the four forest types according to the mean values, with R² and RMSE of 0.503 and 52.335 Mg ha⁻¹, respectively. In conclusion, different variables and suitable models should be considered when estimating the AGB of different forest types. This study could provide a reference for the estimation of forest AGB based on remote sensing in complex terrain areas with a high degree of forest heterogeneity.

Recent archeobotanical work has shed light on prehistoric food globalization across the Eurasian landmass; however, much less research has focused on the foodways of the historical cities and settlements found throughout Central Asia on various portions of the ‘Silk Road’. Here, we present archeobotanical and isotopic results from recent excavations at Shichengzi, a Han dynasty (202 BC–AD 220) military garrison. Our archeobotanical results recovered from 11 samples reveal that four types of cereals, naked barley, wheat, common millet, and foxtail millet, were the most common crops at the site. Naked barley, a drought and cold resistant crop, comprised 79% of the crop assemblage recovered from Shichengzi, and the rest of the assemblage is composed of wheat and millet. The reliance on drought resistant crops indicates that people at Shichengzi oriented their agricultural strategy toward mitigating environmental risks. In addition, our isotopic analyses (δ¹³C and δ¹⁵N) of charred cereal grains ( n = 22), animal and human remains ( n = 12) recovered from excavations at Shichengzi suggests that the δ¹⁵N values of cereals were enriched by human or livestock dung. Moreover, the calculated Δ¹³C values from Shichengzi suggest that farmers preferentially planted their crops in wide areas that would have received a large amount of water available on the northern piedmont of the Tianshan mountains. Our research contributes to the growing understanding of the diversity of agricultural strategies used along the Silk Road.

The uncertainty from the under-estimation and over-estimation of forest aboveground biomass (AGB) is an urgent problem in optical remote sensing estimation. In order to more accurately estimate the AGB of Pinus densata forests in Shangri-La City, we mainly discuss three non-parametric models—the artificial neural network (ANN), random forests (RFs), and the quantile regression neural network (QRNN) based on 146 sample plots and Sentinel-2 images in Shangri-La City, China. Moreover, we selected the corresponding optical quartile models with the lowest mean error at each AGB segment to combine as the best QRNN (QRNNb). The results showed that: (1) for the whole biomass segment, the QRNNb has the best fitting performance compared with the ANN and RFs, the ANN has the lowest R² (0.602) and the highest RMSE (48.180 Mg/ha), and the difference between the QRNNb and RFs is not apparent. (2) For the different biomass segments, the QRNNb has a better performance. Especially when AGB is lower than 40 Mg/ha, the QRNNb has the highest R² of 0.961 and the lowest RMSE of 1.733 (Mg/ha). Meanwhile, when AGB is larger than 160 Mg/ha, the QRNNb has the highest R² of 0.867 and the lowest RMSE of 18.203 Mg/ha. This indicates that the QRNNb is more robust and can improve the over-estimation and under-estimation in AGB estimation. This means that the QRNNb combined with the optimal quantile model of each biomass segment provides a method with more potential for reducing the uncertainties in AGB estimation using optical remote sensing images.

Comparing algorithms are crucial for enhancing the accuracy of remote sensing estimations of forest biomass in regions with high heterogeneity. Herein, Sentinel 2A, Sentinel 1A, Landsat 8 OLI, and Digital Elevation Model (DEM) were selected as data sources. A total of 12 algorithms, including 7 types of learners, were utilized for estimating the aboveground biomass (AGB) of Pinus yunnanensis forest. The results showed that: (1) The optimal algorithm (Extreme Gradient Boosting, XGBoost) was selected as the meta-model (referred to as XGBoost-stacking) of the stacking ensemble algorithm, which integrated 11 other algorithms. The R² value was improved by 0.12 up to 0.61, and RMSE was decreased by 4.53 Mg/ha down to 39.34 Mg/ha compared to the XGBoost. All algorithms consistently showed severe underestimation of AGB in the Pinus yunnanensis forest of Yunnan Province when AGB exceeded 100 Mg/ha. (2) XGBoost-Stacking, XGBoost, BRNN (Bayesian Regularized Neural Network), RF (Random Forest), and QRF (Quantile Random Forest) have good sensitivity to forest AGB. QRNN (Quantile Regression Neural Network), GP (Gaussian Process), and EN (Elastic Network) have more outlier data and their robustness was poor. SVM-RBF (Radial Basis Function Kernel Support Vector Machine), k-NN (K Nearest Neighbors), and SGB (Stochastic Gradient Boosting) algorithms have good robustness, but their sensitivity was poor, and QRF algorithms and BRNN algorithm can estimate low values with higher accuracy. In conclusion, the XGBoost-stacking, XGBoost, and BRNN algorithms have shown promising application prospects in remote sensing estimation of forest biomass. This study could provide a reference for selecting the suitable algorithm for forest AGB estimation.

It is essential to analyze the spatial autocorrelation and heterogeneity of aboveground biomass (AGB). But it is difficult to accurately describe due to the lack of data in clear-cutting plots. Thus, measuring the AGB directly in a clear-cutting plot can provide a reference for accurately describing the spatial variation. Therefore, a 0.3-hectare clear-cutting sample plot of Pinus kesiya var. langbianensis natural forest was selected, and the AGB was calculated by each component. The intra-group variance was quantitatively described in terms of spatial heterogeneity, and the spatial autocorrelation was explored by global and local Moran’s I. The results indicated that (1) there was different spatial heterogeneity for the different trees and organs. The intra-group variance tended to be stable after 20 m for P. kesiya var. langbianensis (PK) and other upper trees (UPs) and after 10 m for the other lower trees (LTs). (2) The spatial autocorrelation of AGB and wood biomass was similar, while the bark biomass and foliage biomass were consistent. PK and other UPs also exhibited strong spatial autocorrelation, with maximum Moran’s I values of 0.1537 and 0.1644, respectively. (3) There was spatial heterogeneity in the different components except for the bark of PK. The lowest spatial heterogeneity was found for LT.

Improving the precision of aboveground biomass (AGB) estimation in lowland tropical forests is crucial to enhancing our understanding of carbon dynamics and formulating climate change mitigation strategies. This study proposes an AGB estimation method for lowland tropical forests in Xishuangbanna, which include various vegetation types, such as Pinus kesiya var. langbianensis, oak, Hevea brasiliensis, and other broadleaf trees. In this study, 2016 forest management inventory data are integrated with remote sensing variables from Landsat 8 OLI (L8) and Sentinel 2A (S2) imagery to estimate forest AGB. The forest age and aspect were utilized as stratified variables to construct the random forest (RF) models, which may improve the AGB estimation accuracy. The key findings are as follows: (1) through variable screening, elevation was identified as the main factor correlated with the AGB, with texture measures derived from a pixel window size of 7 × 7 perform best for AGB sensitivity, followed by 5 × 5, with 3 × 3 being the least effective. (2) A comparative analysis of imagery groups for the AGB estimation revealed that combining L8 and S2 imagery achieved superior performance over S2 imagery alone, which, in turn, surpassed the accuracy of L8 imagery. (3) Stratified models, which integrated aspect and age variables, consistently outperformed the unstratified models, offering a more refined fit for lowland tropical forest AGB estimation. (4) Among the analyzed forest types, the AGB of P. kesiya var. langbianensis forests was estimated with the highest accuracy, followed by H. brasiliensis, oak, and other broadleaf forests within the RF models. These findings highlight the importance of selecting appropriate variables and sensor combinations in addition to the potential of stratified modeling approaches to improve the precision of forest biomass estimation. Overall, incorporating stratification theory and multi-source data can enhance the AGB estimation accuracy in lowland tropical forests, thus offering crucial insights for refining forest management strategies.