Injection water temperature is often different from that of the reservoir during water injection development in the tight reservoir. Temperature change causes different fluid properties and oil-water interface properties, which further affects the imbibition process. In this paper, a matrix-fracture non-isothermal oil-water imbibition flow model in tight reservoirs is established and solved by the finite element method based on the phase-field method. The ideal inhomogeneous rock structure model was used to study the influence of a single factor on the imbibition. The actual rock structure model was used to study the influence of temperature. The mechanism of temperature influence in the process of imbibition is studied from the micro-level. It is found that the imbibition of matrix-fracture is a process in which the water enters the matrix along with the small pores, and the oil is driven into the macropores and then into the fractures. Temperature affects the imbibition process by changing the oil-water contact angle, oil-water interfacial tension, and oil-water viscosity ratio. Reducing oil-water contact angle and oil-water viscosity ratio and increasing oil-water interfacial tension are conducive to the imbibition process. The increase in injection water temperature is usually beneficial to the occurrence of the imbibition. Moreover, the actual core structure imbibition degree is often lower than that of the ideal core structure. The inhomogeneous distribution of rock particles has a significant influence on imbibition. This study provides microscale theoretical support for seeking reasonable injection velocity, pressure gradient, injection temperature, and well-shutting time in the field process. It provides a reference for the formulation of field process parameters.