Cholecystolithiasis is among the most prevalent gastrointestinal disorders requiring surgical intervention, and iatrogenic damage to the bile tree is a severe complication. We aimed to present the frequency of bile duct injuries and how our facility handles these complications. We retrospectively analyzed bile duct injuries in patients undergoing surgery. We concentrated on factors such as sex, age, indications for surgery, type of surgery, primary procedure, bile tree injury, repair, and timing as well as early and late complications. There were 22 cases of bile duct injury in the studied material, primarily affecting women—15 individuals (68.2%). Eleven cases (45.7%) of acute cholecystitis were the primary reason for surgery, and an injury to the common bile duct that extended up to 2 cm from the common hepatic duct was the most common complication (European Association for Endoscopic Surgery grade 2). Roux-en-Y hepaticojejunostomy was the most common repair procedure in 14 cases (63.6%). Eleven patients (50%) experienced early complications following reconstruction surgery, whereas five patients (22.7%) experienced late complications. An annual mortality rate of 22.7% (five patients) was observed. Iatrogenic bile duct injury is a severe complication of surgical treatment for cholecystolithiasis. Reconstruction procedures are characterized by high complication rates and high mortality.

Background: Colorectal cancer (CRC) remains a major global health problem, with rising incidence among younger individuals. The implementation of next-generation sequencing (NGS) has enabled comprehensive multigene analysis to identify cancer-predisposing variants and molecular alterations in tumors. However, data on age-related genetic differences in CRC from Central and Eastern European populations, including Poland, remain limited. Methods: This study aimed to explore molecular differences in CRC between patients aged ≤50 and >50 years in a Polish cohort. Tumor DNA was extracted from formalin-fixed, paraffin-embedded (FFPE) tissue samples obtained from 54 treatment-naive patients. Targeted sequencing of hot spot regions of 50 genes with known association to cancer was performed using an AmpliSeq for Illumina Cancer Hotspot Panel v2. Results: Variant frequencies in younger vs. older patients were: TP53 (71.4% vs. 57.6%), APC (57.1% vs. 45.5%), KRAS (28.1% vs. 72.7%), NRAS (28.6% vs. 0%), SMAD4 (9.5% vs. 12.1%), PIK3CA (14.3% vs. 24.2%), and FBXW7 (4.8% vs. 14.7%). Co-occurrence of APC/KRAS/TP53 variants was observed in 20% of cases. KRAS mutations were significantly more frequent in older patients (p-value = 0.001), while NRAS mutations occurred exclusively in younger patients (29% vs. 0%, p = 0.021). Overall, 46% of patients exhibited multiple gene alterations (≥3 mutations). Notably, IDH1 and CTNNB1 variants were found only in patients with better prognosis, whereas TP53 variants were nearly five times more frequent in patients with worse outcomes. Conclusions: Multigene panel sequencing revealed distinct age-related molecular patterns in CRC. Younger patients were more likely to harbor NRAS variants, whereas KRAS alterations predominated in older individuals. These findings underscore the relevance of NGS-based multigene profiling for risk stratification and personalized therapy in colorectal cancer.

Background. Colorectal cancer (CRC) remains a major global public health problem, particularly in Western countries. The imple-mentation of next-generation sequencing (NGS) has enabled multigene panel analysis to identify cancer-predisposing variants and characterize molecular alterations in tumors. To investigate molecular differences in CRC between patients younger than 50 years and those older than 50 years. Methods. Inclusion criteria were the absence of prior radio- or chemotherapy and availability of DNA suitable for NGS. Patients were divided into two age groups: ≤50 years and >50 years. DNA was extracted from FFPE tissue samples. Targeted sequencing was performed using an Illumina hotspot cancer panel comprising 50 genes (700 amplicons). Results. Mutation frequencies in younger vs. older patients were: TP53 (76% vs. 64%), APC (57% vs. 45%), KRAS (43% vs. 73%), NRAS (29% vs. 0%), SMAD4 (9% vs. 15%), PIK3CA (14% vs. 33%), FBXW7 (5% vs. 15%). Co-occurrence of APC/KRAS/TP53 mutations was observed in 20% of cases. KRAS mutations were significantly more frequent in older patients (p=0.001), while NRAS mutations occurred exclusively in younger patients (29% vs. 0%, p=0.021). Overall, 46% of patients exhibited multiple gene alter-ations (≥3 mutations). Notably, IDH1 and CTBX1 mutations were found only in patients with better prognosis, whereas TP53 mu-tations were nearly twice as common in patients with worse outcomes. Conclusions. Multigene panel sequencing revealed distinct age-related molecular patterns in CRC. Younger patients were more likely to harbor NRAS mutations, whereas KRAS alterations predominated in older individuals. These findings underscore the relevance of NGS-based multigene profiling for risk stratification and personalized therapy in colorectal cancer.

Colorectal Cancer CRC is the third most common cancer worldwide, with 1 2 million new cases diagnosed annually and the fourth leading cause of cancer related deaths.

INPLASY202450009 22-G or 25-G needle: which one to use in diagnostics of solid pancreatic lesions? A systematic review and meta-analysis Keywords: EUS; needle; FNA; pancreas; prospective; 22G; 25G.…