In recent years, much research has focused on studying the damage caused by microplastics to the ecological environment and human health. Indeed, MPs are often consumed by shellfishes and zooplanktons due to their similarity in size to POM (particular organic matter). Especially in zooplankton, the accumulation of MPs in the body affects the reproductive system and the growth rate of juveniles. Moreover, toxins derived from MPs are continuously accumulated in predators of zooplankton and impact the whole ecosystem across the food chain. In this work, we found that even though MPs were internalized by and adherent around Daphnia magna, there were no significant differences in the survival rate of their adults and offspring. However, the population of ovigerous adults under high MPs exposure for 7 days decreased significantly, suggesting an extension of the days of sexual maturity in D. magna. The removal of MPs after 7 days’ MPs treatment resulted in an increase in D. magna juveniles and neonates which indicated their growth was reduced or inhibited in the MPs environment. Overall, the uptake of MPs led to negative effects on population reproduction and the growth of offspring in D. magna.

We previously reported that Aurora-A and the hNinein binding protein AIBp facilitate centrosomal structure maintenance and contribute to spindle formation. Here, we report that AIBp also interacts with Plk1, raising the possibility of functional similarity to Bora, which subsequently promotes Aurora-A–mediated Plk1 activation at Thr210 as well as Aurora-A activation at Thr288. In kinase assays, AIBp acts not only as a substrate but also as a positive regulator of both Aurora-A and Plk1. However, AIBp functions as a negative regulator to block phosphorylation of hNinein mediated by Aurora-A and Plk1. These findings suggest a novel AIBp-dependent regulatory machinery that controls mitotic entry. Additionally, knockdown of hNinein caused failure of AIBp to target the centrosome, whereas depletion of AIBp did not affect the localization of hNinein and microtubule nucleation. Notably, knockdown of AIBp in HeLa cells impaired both Aurora-A and Plk1 kinase, resulting in phenotypes with multiple spindle pole formation and chromosome misalignment. Our data show that depletion of AIBp results in the mis-localization of TACC3 and ch-TOG, but not CEP192 and CEP215, suggesting that loss of AIBp dominantly affects the Aurora-A substrate to cause mitotic aberrations. Collectively, our data demonstrate that AIBp contributes to mitotic entry and bipolar spindle assembly and may partially control localization, phosphorylation, and activation of both Aurora-A and Plk1 via hNinein during mitotic progression.

Duckweed (Lemna aequinoctialis) is one of the smallest flowering plants in the world. Due to its high reproduction rate and biomass, duckweeds are used as biofactors and feedstuff additives for livestock. It is also an ideal system for basic biological research and various practical applications. In this study, we attempt to establish a micropropagation technique and Agrobacterium-mediated transformation in L. aequinoctialis. The plant-growth regulator type and concentration and Agrobacterium-mediated transformation were evaluated for their effects on duckweed callus induction, proliferation, regeneration, and gene transformation efficiency. Calli were successfully induced from 100% of explants on Murashige and Skoog (MS) medium containing 25.0 μM 2,4-dichlorophenoxyacetic acid (2,4-D) and 2.0 μM thidiazuron (TDZ). MS medium containing 4.5 μM 2,4-D and 2.0 μM TDZ supported the long-lasting growth of calli. Fronds regenerated from 100% of calli on Schenk and Hildebrandt (SH) medium containing 1.0 μM 6-benzyladenine (6-BA). We also determined that 200 μM acetosyringone in the cocultivation medium for 1 day in the dark was crucial for transformation efficiency (up to 3 ± 1%). Additionally, we propose that both techniques will facilitate efficient high-throughput genetic manipulation in Lemnaceae.

Aquaponic systems are made up of hydroponic beds and recycled aquaculture systems. The significant elements that determine how effectively an aquaponic system operates are the nitrogen cycle (nitrification) and the phosphorus cycle (phosphate). Because some research indicates that aquaponics systems are primarily deficient in phosphorus, phosphate-enriched nutrients were added to raise the phosphorus levels. During an eight-week experimental period, the effects of water quality parameters and microbiology, animal and plant growth performance, chlorophyll compounds in lettuce, and the bacterial community were analyzed. Phosphate concentration (1.604 ± 1.933 mg L⁻¹) and ammonia-oxidizing bacteria (AOB) (1.19 × 10² ± 1.30 CFU mL⁻¹) give significant positive reactions to the added nutrients. However, the prawn survival rate (17.00 ± 0.63%) showed a significantly negative response to nutrition modification containing phosphate, and the percentage of bacterial pathogens became more dominant (pathogen 40.51%; N_bacteria 35.05%; probiotics 24.44%). This study shows that adding phosphate increases phosphorus levels in an aquaponics system and changes the microbial community and species growth performance.

Endosymbiosis between dinoflagellates and cnidarian hosts first occurred more than 200 million years ago; however, symbiosis-specific genes and cellular processes involved in the establishment, maintenance, and breakdown of endosymbiosis remain unclear. Therefore, this study aimed to identify the zooxanthellal genes associated with the aforementioned biological processes during endosymbiosis in Aiptasia-Symbiodinium endosymbionts. Here, zooxanthellae isolates were treated with 0.02% SDS to decrease potential host RNA contamination and to enhance the identification of novel symbiosis/nonsymbiosis-associated differentially expressed zooxanthellal genes through suppressive subtractive hybridization (SSH) and next-generation sequencing (NGS) methods. Consequently, among 214 symbiosis-specific transcripts identified herein that displayed identity to only 5.6% of host-derived transcripts, 64% were well-known functional genes. In the nonsymbiotic stage, 181 differentially expressed transcripts were identified, of which 64.1% belonged to well-known functional genes. BLAST revealed that 8 categories of cellular processes were significantly induced in symbiotic or nonsymbiotic zooxanthellae. Together with the results of quantitative analysis, the results revealed that photosynthesis, flagellate biosynthesis and motility, stress-induced responses, cell wall biosynthesis, starch synthesis and transport, lipid biosynthesis and metabolism, host/symbiont immune response, intercellular communication, cell growth, and cell cycle regulation were the major cellular processes occurring in symbiotic/nonsymbiotic stages. The present results provide insights into the mechanisms involved in regulating the different physiological processes in symbiotic/nonsymbiotic zooxanthellae and may guide future studies.