Yee Hwee Lim

Dr. Yee Hwee Lim

orcid

Research Focus & Metrics

Academic Career Length

22Years (2005-Present)

(Calculated once Scilit publication data is synced: current year − year of first publication + 1)

Representative Publications

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10 April 2017 in Nature Chemical Biology
Verified
Journal Article
Open access
PDF file
MZMingzi Zhang
Mingzi Zhang
FWFong Tian Wong
Fong Tian Wong
YWYAJIE WANG
YAJIE WANG
SLShangwen Luo
Shangwen Luo
Yee Hwee LimYee Hwee Lim
Yee Hwee Lim
HZHuimin Zhao
Huimin Zhao
Most microbial biosynthetic gene clusters are inactive under laboratory culture conditions. A CRISPR–Cas9 genome-editing approach in Streptomyces species enables the targeted activation of silent gene clusters and production of encoded natural products. Here we report an efficient CRISPR–Cas9 knock-in strategy to activate silent biosynthetic gene clusters (BGCs) in streptomycetes. We applied this one-step strategy to activate multiple BGCs of different classes in five Streptomyces species and triggered the production of unique metabolites, including a novel pentangular type II polyketide in Streptomyces viridochromogenes. This potentially scalable strategy complements existing activation approaches and facilitates discovery efforts to uncover new compounds with interesting bioactivities.

25 May 2012 in Angewandte Chemie International Edition
Verified
Journal Article
Closed access
PDF file
ZGZhanghua Gao
Zhanghua Gao
Yee Hwee LimYee Hwee Lim
Yee Hwee Lim
MTMatthew Tredwell
Matthew Tredwell
LLLei Li
Lei Li
SVStefan Verhoog
Stefan Verhoog
VGVéronique Gouverneur
Véronique Gouverneur

14 March 2007 in Journal of the American Chemical Society
Verified
Journal Article
Closed access
KNK. C. Nicolaou
K. C. Nicolaou
Yee Hwee LimYee Hwee Lim
Yee Hwee Lim
JPJared L. Piper
Jared L. Piper
CPCharles D. Papageorgiou
Charles D. Papageorgiou

27 June 2018 in ChemBioChem
Verified
Journal Article
Closed access
PDF file
Yee Hwee LimYee Hwee Lim
Yee Hwee Lim
FWFong Tian Wong
Fong Tian Wong
WYWan Lin Yeo
Wan Lin Yeo
KCKuan Chieh Ching
Kuan Chieh Ching
YLYi Wee Lim
Yi Wee Lim
HZHuimin Zhao
Huimin Zhao

23 April 2009 in Angewandte Chemie International Edition
Verified
Journal Article
Closed access
PDF file
KNK. C. Nicolaou
K. C. Nicolaou
Yee Hwee LimYee Hwee Lim
Yee Hwee Lim
JBJochen Becker
Jochen Becker

8 December 2005 in Angewandte Chemie International Edition
Verified
Journal Article
Closed access
PDF file
KNK. C. Nicolaou
K. C. Nicolaou
Yee Hwee LimYee Hwee Lim
Yee Hwee Lim
CPCharles D. Papageorgiou
Charles D. Papageorgiou
JPJared L. Piper
Jared L. Piper