Tourism is an activity that contributes positively to economic growth. Although tourism should be supported because of its benefits for a region’s development, it can place a strain on the environment. Integrating the principles of sustainable development into tourism is essential to ensuring its long-term viability while protecting natural and cultural resources. The purpose of this study is to determine the extent of tourism pressure, its trends, and its impact on sustainable development in three major rural tourist regions of Romania, namely Humor, Câmpulung Moldovenesc, and Dorna, located in the historical province of Bucovina, Suceava County. An analysis of the trends in the three evaluation indicators (density, duration, and pressure level) at the rural region level (Humor Region, Câmpulung Moldovenesc Region, and Dorna Region) is warranted, as these regions are the main centers of attraction and development for rural tourism in Suceava County. Between 2001 and 2023, the density indicator (arrivals/km2) showed significant increases in all three rural regions analyzed—over 6000% in Humor, 2200% in Dorna, and 2000% in Câmpulung Moldovenesc, significantly amplifying the pressure on these three areas; the duration indicator (overnight stays/km2) recorded considerable increases of over 7400% in Humor, 2400% in Dorna, and 2250% in Câmpulung Moldovenesc, proving to be a much more severe pressure indicator than the density indicator; and the pressure level indicator (arrivals/resident population) increased substantially, by over 8000% in Humor, 2100% in Dorna, and 2000% in Câmpulung Moldovenesc, indicating profound sociocultural pressure on the rural communities analyzed. The importance of this research and the originality of this study are closely linked to an understanding of the evolution and intensity of tourism pressure in the three regions analyzed, with a view to informing future strategies for sustainable rural development. An analysis of the environmental pressure caused by tourism in the three regions of Bucovina for the period 2001–2023 reflects a shift from a localized and negligible impact to an increasingly significant pressure. Currently, the regions face the challenge of balancing rapid economic development with the critical need to preserve their unique heritage. Identifying increases in tourist pressure can facilitate the implementation of effective mitigation measures, which are essential for ensuring the sustainability of tourism-related activities in the most popular tourist regions of Bucovina.