Accompanying the thawing permafrost expected to result from the climate change, microbial decomposition of the massive amounts of frozen organic carbon stored in permafrost is a potential emission source of greenhouse gases, possibly leading to positive feedbacks to the greenhouse effect. In this study, the community composition of archaea in stratigraphic soils from an alpine permafrost of Qinghai-Tibetan Plateau was investigated. Phylogenic analysis of 16S rRNA sequences revealed that the community was predominantly constituted byCrenarchaeotaandEuryarchaeota. The active layer contained a proportion ofCrenarchaeotaat 51.2%, with the proportion ofEuryarchaeotaat 48.8%, whereas the permafrost contained 41.2%Crenarchaeotaand 58.8%Euryarchaeota, based on 16S rRNA gene sequence analysis. OTU1 and OTU11, affiliated to Group 1.3b/MCG-A withinCrenarchaeotaand the unclassified group withinEuryarchaeota, respectively, were widely distributed in all sediment layers. However, OTU5 affiliated to Group 1.3b/MCG-A was primarily distributed in the active layers. Sequence analysis of the DGGE bands from the 16S rRNAs of methanogenic archaea showed that the majority of methanogens belonged toMethanosarcinalesandMethanomicrobialesaffiliated toEuryarchaeotaand the uncultured ZC-I cluster affiliated toMethanosarcinalesdistributed in all the depths along the permafrost profile, which indicated a dominant group of methanogens occurring in the cold ecosystems.

Wetlands are an important source of atmospheric methane (CH₄) and are sensitive to global climate change. Alpine swamp meadows, accounting for ~50% of the natural wetlands on the Qinghai-Tibet Plateau, were considered one of the most important ecosystems. Methanogens are important functional microbes that perform the methane producing process. However, the response of methanogenic community and the main pathways of CH₄ production to temperature rise remains unknown in alpine swamp meadow at different water level in permafrost wetlands. In this study, we investigated the response of soil CH₄ production and the shift of methanogenic community to temperature rise in the alpine swamp meadow soil samples with different water levels collected from the Qinghai-Tibet Plateau through anaerobic incubation at 5°C, 15°C and 25°C. The results showed that the CH₄ contents increased with increasing incubation temperature, and were 5–10 times higher at the high water level sites (GHM1 and GHM2) than that at the low water level site (GHM3). For the high water level sites (GHM1 and GHM2), the change of incubation temperatures had little effect on the methanogenic community structure. Methanotrichaceae (32.44–65.46%), Methanobacteriaceae (19.30–58.86%) and Methanosarcinaceae (3.22–21.24%) were the dominant methanogen groups, with the abundance of Methanotrichaceae and Methanosarcinaceae having a significant positive correlation with CH₄ production (p < 0.01). For the low water level site (GHM3), the methanogenic community structure changed greatly at 25°C. The Methanobacteriaceae (59.65–77.33%) was the dominant methanogen group at 5°C and 15°C; In contrast, the Methanosarcinaceae (69.29%) dominated at 25°C, and its abundance showed a significant positive correlation with CH₄ production (p < 0.05). Collectively, these findings enhance the understanding of methanogenic community structures and CH₄ production in permafrost wetlands with different water levels during the warming process.

Many cold seeps and gas hydrate areas have not been discovered beside the Beikan basin in the southern South China Sea (SCS), and their characteristics and histories also remain poorly known. Here we describe authigenic minerals and the carbon and oxygen isotopic composition of planktonic foraminifera Globigerinoides ruber from sediment core 2PC, recovered from the gas hydrate zone of the Nansha Trough, southern SCS, to elucidate its history of dynamic cold seepage. We infer that the occurrence of authigenic gypsum crystals and pyrite concretions, and anomalously negative δ¹³C values of Globigerinoides ruber, reflect paleo-methane seepage. Two major methane release events were identified, based on remarkable excursions in foraminifera δ¹³C at depths of 150–250 cm and 350–370 cm. Euhedral gypsum crystals and tubular pyrite concretions co-occur with extremely negative planktonic foraminifera δ¹³C values, indicating a shift in the sulfate methane transition zone and a change in the methane flux. Our data suggest that authigenic mineral assemblages and δ¹³C values of planktonic foraminifera provide a valuable tool in elucidating the characteristics of dynamic methane seepage in a marine environment.