Background: Seafood contamination with Salmonella enterica is not only a public health concern, but can also lead to economic losses due to import rejections. Continuous monitoring of seafood for Salmonella is necessary for risk assessment and to establish suitable control measures. Objective: The objective of this study was to determine the extent of Salmonella contamination of seafood in fish landing centers and retails markets. Methods: In this study, we analyzed seafood samples from three fish landing centers and seven retail markets of Mumbai, India for S. enterica contamination. Salmonella was isolated using multiple selective enrichment broths and selective agars, and identified by conventional biochemical tests followed by polymerase chain reaction (PCR). Results: Of 82 samples of seafood, comprising both finfish and shellfish, Salmonella was detected in 17 (20.7%) finfish samples. None of the shellfish samples (shrimps, clams, and cephalopods) were found to be contaminated with Salmonella. Samples from both landing centers and fish markets, as well as from pelagic and demersal sources, harbored Salmonella. Source-wise analysis showed that 11 of 57 (19.3%) samples from retail fish markets and 6 of 25 (24%) samples from fish landing centers were contaminated with Salmonella. Conclusions: The study emphasizes the need to implement measures to prevent anthropogenic contamination of coastal waters and improve the hygiene of retail fish markets in Mumbai. Highlights: Seafood from landing centers and retail markets, as well as from pelagic and demersalhabitats were similarly contaminated with Salmonella. Fish species widely consumed in the region of this study were found highly contaminated with Salmonella.

The objective of the present study was to determine the incidence of STEC in fresh seafood in Mumbai, India, and to characterize STEC with respect to their virulence determinants

Background and Aim: All serotypes of Salmonella enterica are considered potentially pathogenic. However, the non-typhoidal Salmonella (NTS) serotypes vary considerably in terms of pathogenicity and the severity of infections. Although diverse serotypes of NTS have been reported from tropical seafood, their sources, physiological characteristics, and virulence potentials are not well understood. This study aimed to compare the physiological characteristics of selected serovars of Salmonella from seafood and investigate possible variations in the distribution of known genes within the pathogenicity islands. Materials and Methods: A series of biochemical tests, including carbohydrate fermentation and amino acid decarboxylation tests were performed to physiologically compare the isolates. The genetic characterization with respect to putative virulence genes was done by screening for genes associated with Salmonella pathogenicity island (SPI) I– V, as well as the toxin- and prophage-associated genes by polymerase chain reaction. Results: Irrespective of serotypes, all the isolates uniformly harbored the five SPIs screened in this study. However, some virulence genes, such as the avrA, sodC, and gogB were not detected in all Salmonella isolates. The biochemical profiles of Salmonella serotypes were highly conserved except for variations in inositol fermentation and citrate utilization. All the isolates of this study were weak biofilm formers on polystyrene surfaces. Conclusion: The pathogenicity profiles of environmental NTS isolates observed in this study suggest that they possess the virulence machinery necessary to cause human infections and therefore, urgent measures to contain Salmonella contamination of seafood are required to ensure the safety of consumers. Keywords: biofilm, invasion, non-typhoidal Salmonella, Salmonella pathogenicity islands, seafood, virulence.

A brief review was done based on the literatures available and the findings from these research studies are used to evaluate its effectiveness on rural livelihood upliftment, poverty alleviation and implications for education and further research. Rural development in India is one of the most important factors for the growth of the Indian economy. Even after seventy years post-independence, India has not been able to come under “developed nation”, instead the nation is still developing. Public administration, governed by bureaucratic structures that dominated the twentieth century has failed to respond to the changing requirements of the present times. ICT plays a prominent role in strengthening societal development and helps to speed up the developmental process and can also bridge gaps between the educationally and technologically backward and forward sections of society. Various e-governance projects have attempted to improve the reach, enhance the base, minimize the processing costs, increase transparency and reduce the cycle times. For example, the introduction of computer and e-chaupal, radio farm forum, community radio, and ministry of rural development mobile apps such as shramik bandhu, janmanrega, meri sadak app, etc., are some of the initiatives by the government that have up to some extent been able to bring the rural population in contact with technologies. There are great opportunities for ICT application that help in democratic and sustainable development of rural India. This review mainly focuses on various ICT-based applications and their role in the Rural Development of India.

The fastest–growing animal food-producing agricultural sector in the world is aquaculture, which accounts for almost half of the world’s food fish production.However, the expansion of high-density fish populations also brings forth a challenge–the rapid transmission and spread of infectious agents including several viral, bacterial, and parasitic diseases. Expansion and successful development of sustainable aquaculture practice and increasing production largely depends upon the prevention and control of outbreaks of several emerging and re-emerging infectious diseases which can result in economic loss, food safety hazards, and environmental hazards. Vaccination strategies have become highly effective and economical in protecting the health of fish and other aquaculture organisms from various infectious diseases such as edwardsiellosis, motile aeromonas septicemia (MAS), Tilapia Lake Virus (TiLV) disease, infectious salmon anemia (ISA), vibriosis, and white spot disease etc. An ideal vaccine is expected to be safe, effective, economical, and easily administered. Most of the available fish vaccines are empirically designed vaccines based on inactivated or live attenuated bacterin vaccines. Novel advances in the fields of immunology, biotechnology, and molecular biology have led to the development in designing novel and effective fish vaccines and the improvement of the existing vaccines to provide sufficient immune protection against diseases. This review investigates the currently available fish vaccines for use in finfish aquaculture against different infectious diseases different mode of vaccine administration with addressing pros and cons in detail. The information was collected from different secondary sources, and then compiled systematically.