Melamine (ML), a chemical substance of high nitrogen content, is used as a food adulterant. Former evidences implied that ML could induce a variety of toxic effects including neurotoxicity and cognitive impairment. Therefore, the aim of this study was to delineate the protective effect of the nootkatone (NK) against ML-induced neural adverse effects. Rats were orally pretreated with NK (5 and 10 mg/kg) prior to the oral administration of ML (700 mg/kg) for a period of 28 days. Our findings unveiled remarkable alleviating effect of NK on MK-induced neurobehavioral disturbance in open field test. Furthermore, NK lessened ML-caused increases in the acetylcholine esterase level in the brain tissue of exposed rats. NK also decreased the neural oxidative stress as represented by elevated levels of SOD, CAT, and GSH along with decreased MDA and NO levels. Upregulated mRNA expression levels of neural NRF-2 and HO-1 were noticed after NK administration. Remarkable anti-inflammatory impact was prominent by decreased neural IL-1β, and TNF-α along with downregulated NF-κB and TLR-4 gene expression levels in NK-treated rats. Noteworthily, pre-treatment with NK decreased the immune reaction of RAGE and HMGB-1 induced by oral ML exposure. Brain histological examination validated the obtained biochemical and molecular results. To sum up, these outcomes reveal that NK successfully alleviated the neural damage induced by ML via blocking of oxidative stress, and inflammatory signaling pathways. Consequently, our study may suggest NK as a new effective therapeutic supplement for treatment of ML-mediated neurotoxicity in rats via inhibition of HMGB-1-RAGE/TLR-4/NF-κB.

Global warming is an unanimously accepted phenomenon by the international scientific community, being already highlighted by the analysis of observational data over long periods of time, with an increase in temperature of over 1 °C. Climate change in Romania is part of the global context, taking into account the regional conditions, with an increasing trend of arid summer. While the link between high temperatures, climate change and rainfall has been modeled in detail, the situation is not the same for plant water accessibility. The period of time between 1898 and the present, corresponding to the annual records of precipitation and evapotranspiration, overlapped with important political and administrative changes in the studied area, and with extensive hydro-amelioration works. The aim of the paper is to statistically follow the evolution over time of precipitation, namely of evapotranspiration measured in Western Romania, which would allow the expression of conclusions regarding the improvement directions of the water regime. In order to follow the evolution in time of these data, the interval of 1898–2019 was divided into three periods: 1898–1950, 1951–1989 and 1990–2019, respectively. The increase in temperature, especially during the vegetation period and the large number of years in which evapotranspiration quantitatively exceeds the precipitation, indicating the need for effective measures to regulate the water balance.

The purpose of this study was to analyze the chemical composition and antimicrobial activity of some thymus populations collected from five different locations in Western Romania. The chemical compositions of the essential oils (EOs) were studied through GC–MS, and the biological activities were evaluated using the microdilution method. The EO yield ranged between 0.44% and 0.81%. Overall, 60 chemical compounds were identified belonging to three chemotypes: thymol (three populations), geraniol (one population) and carvacrol (one population). Thymus vulgaris L. is distinguished by a high content of thymol, while species of spontaneous flora (Th. odoratissimus and Th. pulegioides) contain, in addition to thymol, appreciable amounts of carvacrol and geraniol. The antimicrobial activity of each the five oils was tested on Staphylococcus aureus (ATCC 25923), Streptococcus pyogenes (ATCC 19615), Esherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853), Shigella flexneri (ATCC 12022), Salmonella typhimurium (ATCC 14028), Haemophilus influenzae type B (ATCC 10211), Candida albicans (ATCC 10231) and Candida parapsilopsis (ATCC 22019). The EOs showed biological activity on Gram-positive/Gram-negative/fungal pathogens, the most sensitive strains proving to be S. pyogenes, S. flexneri, S. typhimurium and C. parapsilopsis with an MIC starting at 2 µL EO/100 µL. The species sensitive to the action of Thymus sp. from culture or spontaneous flora are generally the same, but it should be noted that T. odoratissimus has a positive inhibition rate higher than other investigated EOs, regardless of the administered oil concentration. To date, there is no research work presenting the chemical and antimicrobial profiling of T. odoratissimus and the correlations between the antimicrobial potential and chemical composition of wild and cultivated populations of thyme (Thymus sp.) growing in Western Romania.

The provincial government, a separate association of regional communities in the state structure, carries out public tasks that affect the quality of life of the local community and the activities of businesses. Geographical conditions and the effect of socioeconomic factors lead to different levels of development in different regions. The endogenous potential of the local government is the result of a combination of local conditions, determining its opportunities and directions of development. The aim of this article was to assess and identify the spatial differentiation in the relationship between the green economy and the financial situation of voivodeships in Poland using synthetic measures. The choice of variables for the period of 2010–2020 was determined by the availability of the data collected from the regional system in the CSO Local Data Bank. The Technique for Order of Preference by Similarity to the Ideal Solution was used to construct the synthetic measures. Financial resources are the basis for the operation of local government units, conditioning the implementation of their current and developmental tasks. Knowledge of the current state of finances allows authorities to make comparisons with other units and is helpful in making financial decisions. The green economy is one of the paths to sustainable development. The green economy increases the quality of life and social equality while reducing environmental risks and natural resource consumption. As a result of the research procedure, the spatial differentiation of provinces in Poland in 2010–2020 is presented in terms of the level of the synthetic measure of the green economy and financial situation. In 2010, the synthetic measure of the green economy ranged from 0.31 to 0.42, and in 2020, it ranged from 0.40 to 0.53. The synthetic measure of financial risk in 2010 ranged from 0.37 to 0.61, and in 2020, it ranged from 0.40 to 0.77. The measures are shaped by economic activity and working conditions, health, education, leisure and social relations, economic and physical security, and environmental quality. The results of this survey enable local governments to make comparisons. The lessons learned can allow them to identify potential directions for optimizing development policies.

N-nitrosodiethylamine (ND) is an extremely toxic unavoidable environmental contaminant. Copper^II-albumin (CuAB) complex, a newly developed Cu complex, showed antioxidant and anti-inflammatory potential. Hereby, we explored the plausible neuroprotective role of CuAB complex toward ND-evoked neurotoxicity in mice. Twenty-four male mice were sorted into 4 groups (6 mice each). Control group, mice were administered oral distilled water; and CuAB group, mice received CuAB complex at a dose of 817 µg/kg orally, three times weekly. In ND group, ND was given intraperitoneally (50 mg/kg body weight, once weekly for 6 w). CuAB+ND group, mice were administered a combination of CuAB and ND. The brain was quickly extracted upon completion of the experimental protocol for the evaluation of the oxidative/antioxidative markers, inflammatory cytokines, and histopathological examination. Oxidative stress was induced after ND exposure indicated by a reduction in GSH and SOD1 level, with increased MDA level. In addition, decreased expression of SOD1 proteins, Nrf2, and 5-HT mRNA expression levels were noticed. An apoptotic cascade has also been elicited, evidenced by overexpression of Cyt c, Cl. Casp 3. In addition, increased regulation of proinflammatory genes (TNF-α, IL-6, iNOS, Casp1, and NF-κB (p65/p50); besides, increment of protein expression of P-IKBα and reduced expression of IKBα. Pretreatment with CuAB complex significantly ameliorated ND neuronal damage. Our results recommend CuAB complex supplementation because it exerts neuroprotective effects against ND-induced toxicity.

Salinity stress is one of the marked influencing factors on the ecophysiology of aquaculture and is considered an important reason for the retreat of the fish industry. The current study is an endeavor to elucidate the molecular mechanisms that underlie the response to salinity stress in common carp. Fish (Average weight 5 ± 2 g) were randomly distributed into two groups; the 1^st is a control was exposed to tap water (0.2 ppt salinity) and the 2^nd is a treated was exposed to hypersalinity (10 ppt salinity) for five days. Serum biochemical indicators including total protein, albumin, globulins, A/G ratio, blood glucose, cortisone, Na⁺, K⁺, and Cl^- levels were evaluated. Besides, Tumor necrosis factor-α, interleukin-1β, corticotropin-releasing hormone, and catalase enzyme mRNA expression levels were assessed in lymphoid and immunocompetent organs (liver and spleen) and osmoregulatory organs (kidney and gills) by using Real-time qPCR. Hypersalinity adversely affected the biochemical markers; total protein, albumin, and globulins decreased significantly; however, blood glucose, serum cortisol, and sodium markedly increased in fish exposed to hypersalinity compared with the control. In addition, from the molecular point of view, all the evaluated genes were upregulated at a high expression rate in the liver compared with other studied organs after the salinity challenge. On the contrary, hypersalinity modulated the expression of immune-related genes (Tumor necrosis factor-α and interleukin-1β) in the kidney and spleen and upregulated corticotropin-releasing hormone mRNA in all studied organs except gills. In conclusion, the obtained data clarified the molecular and biochemical mechanisms of salinity stress on the liver, kidney, spleen, and gills. Furthermore, it strongly suggests the implication of neural, endocrine, and immune systems in the responsive mechanisms to the salinity stress in carp.