Fritillaria Tourn. ex L. is a genus of Liliaceae including a little more than 150 species occurring in the temperate Holarctic. Fritillaria sonnikovae Shaulo & Erst is the most recently described Siberian species in the genus. In the affinity section of the F. sonnikovae diagnosis, only F. dagana Turcz. and F. roylei Hook. are mentioned. Our study is an original attempt to shed light on the F. sonnikovae origin and its evolutionary relationships with other Fritillaria using nuclear (ITS) and plastid (matK + rps16 + trnH-psbA) DNA markers. Our results showed that F. sonnikovae together with F. dagana and F. maximowiczii Freyn belongs to the North Asian lineage of the Liliorhiza subgenus and produced no evidence supporting relationship between F. sonnikovae and F. roylei. Monophyly of Fritillaria sonnikovae was not reliably confirmed in our study since its close affinity with F. maximowiczii was demonstrated by phylogenetic analysis and morphology. Fritillaria dagana was shown to be a sister to the F. maximowiczii + F. sonnikovae group. Most authors of the present study suggest considering F. sonnikovae as a synonym for F. maximowiczii. In this view, F. sonnikovae may be considered a narrow endemic and one of the light-perianth morphs of F. maximowiczii, which has emerged in the Western Sayan and remained there as a tertiary relict.

The genetic polymorphism of 7 populations of Pulsatilla orientali-sibirica, P. turczaninovii , and their hybrid P. herba-somnii , which grow in Krasnoyarsk and its surroundings, was analyzed. The studied populations have a relatively low level of polymorphism, ranging from 30 % to 51 %. The populations of the same species have a high genetic similarity (92-95 %). The population of P. herba-somnii grows in areas where P. orientali-sibirica and P. turczaninovii species are found together. On a dendrogram of similarity constructed using ISSR markers, individuals of this hybrid are grouped with P. orientali-sibirica , and with P. turczani-novii in phylogenetic analysis. When the studied samples were clustered via STRUCTURE, P. herba-somnii individuals occupied an intermediate position. In phylogenetic analysis of Pulsatilla , the most informative markers are the nuclear ITS - region and the plastid intergenic spacers atpB-rbcL and rpoB-trnC.

Pulsatilla is a genus of ephemeroid grasses characterized by high morphological diversity. The study assessed the genetic polymorphism of 9 populations represented by the species P orientali-sibirica Stepanov, P turczaninovii Krylov and their hybrid P. herba-somnii Stepanov, and P. multifida G. Prizel. As a result of amplification of genomic DNA with 4 ISSR primers, 61 DNA amplicons were obtained. The overall level of polymorphism is 98.36 %. The number of amplified DNA fragments, depending on the primer, varies from 11 (17899A) to 17 (17899 B, HB12). The maximum level of genetic variability was noted for hybrid populations. The coefficient of genetic subdivision (Gst) of the studied populations is 0.4718, which indicates a high level of their differentiation. The dendrogram divides the studied populations into 3 groups, each of which is represented by a separate species, while the hybrid population is combined with P. orientali-sibirica. Phylogenetic analysis of the studied populations combines P orientali-sibirica with P multifida and P turczaninovii with P. herba-somnii. These data demonstrate the intermediate position of the hybrid population of P. herba-somnii. In the study of hybrids, ISSRS are the most informative, since they are evenly distributed across the genome and can show similarities with two parent organisms, whereas chloroplasts are inherited, as a rule, through the maternal line.

Corydalis subjenisseensis (Antipova) is a tuberous ephemeroid characterized by a high morphological diversity. During the research work genetic polymorphism of 7 populations Corydalis subjenisseensis s. l., growing in the southof the Yenisei Siberia were analyzed using ISSR markers. The amplification of genomic DNA with 8 ISSR primers yielded100 DNA amplicons of which 78 were polymorphic. The number of amplified DNA fragments, depending on the primer,varied from 9 (ISSR-17) to 21 (HB14). The maximum level of genetic variation was observed for Western Sayan populations growing in aspen and fir forests. The genetic differentiation among populations (Gst) was 0.2415, indicating a highlevel of differentiation. The similarity dendrogram performed in the TFPGA program shows a division into 2 groups: thefirst group includes the Krasnoyarsk and Khakass populations of C. subjenisseensis, the second group includes the Tanzybei populations, which are characterized by a high level of polymorphism. A similar structure is observed when buildingclusters using the Bayesian approach. 69 genotypes are divided into a maximum of 7 genetic clusters. Among the populations of the Tanzybei, individuals of presumably hybridogenic origin are found, grouped around two centers of “attraction”.