Low-molecular-weight organic acids (LMWOAs) are essential components of rice roots exudates and an important source of soil organic carbon. The chemical-microbial pathway by which LMWOA affects arsenic (As) cycling in the rhizosphere of paddy soils is still unclear. In this study, three typical LMWOAs (acetic acid (AA), oxalic acid (OA), and citric acid (CA)) in rice root exudates were added to As-contaminated soil at a concentration of 10 mM, mimicking the rhizosphere environment. The results showed that the addition of AA and OA inhibited the mobilization of As in the rhizosphere soil. After 14 days of incubation, the content of As in the porewater of AA and OA decreased by 40% and 22%, respectively, compared with the control. AA hindered the mobilization of As in soil via promoting the formation of secondary minerals. The addition of OA inhibits the mobilization of As via increasing the proportion of As (V) in porewater and promoting the formation of secondary minerals in soil. In addition, OA addition not only significantly increased the aioA gene abundance but also notably enriched the microorganisms containing As (III) methylation functional genes (arsM). The addition of CA greatly expedited the release of As from the soil solid phase through the solubilization of Fe/Mn minerals via the effects of both soil chemistry and microbial action. Furthermore, linear discriminant analysis effect size (LEfSe) revealed the possibility that bacteria such as Burkholderia, Magnetospirillum, and Mycobacterium were involved in the reduction or methylation of As in the rhizosphere of paddy soil. This study revealed the internal causes of LMWOAs regulating As transformation and mobilization in flooded paddy soil and provided theoretical support for reducing As accumulation in rice by breeding rice varieties with high AA and OA secretions.

Anhui Province is located in the eastern China, in the middle and lower reaches of the Yangtze River and Huaihe River, and contains three major basins, i.e., the Yangtze River, Huaihe River, and Xin’an River basins. Based on the statistical data of livestock and poultry quantity and farmland area in Anhui Province in 2019, ArcGIS was used to analyze the spatial distribution characteristics of the livestock and poultry excreta (LPE) pollutants in Anhui Province in order to explore the potential pollution risk posed by livestock and poultry farming to the farmland and the water environment in Anhui Province. The equivalent pollution load method was adopted to compare and assess the release of LPE pollutants in various cities and to analyze the causes. Through the calculation of the farmland carrying capacity load and early warning value of LPE and the water load of livestock and poultry pollutant release based on the equivalent pollution index method, a comprehensive assessment of the potential pollution risk posed by livestock and poultry farming on farmland and the water environment in Anhui Province was carried out. In this study, the spatial distribution of the livestock and poultry pollution in Anhui Province was analyzed, the effect of the pollution load of the livestock and poultry on the cultivated land and water environment was evaluated, and suggestions for environmental protection measures are provided. The results of this study revealed that the total pig equivalent of the livestock and poultry farming in Anhui Province was 55,068,400 and the LPE output was 47,778,600 t in 2019. The LPE pollutant output was 1,707,700 t, and the total release was 510,400 t. The release of pollutant chemical oxygen demand (COD) accounted for 71.67% of the total release. The average farmland load of the pig manure equivalent was 8.09 t/hm² in the province. The average pollutant diffusion concentration of in the water was 31.63 mg/L. The average equivalent pollution index of LPE was 5.23, indicating a mild pollution impact on the water environment. Overall, the spatial distribution of the LPE pollutant output and pig manure equivalent farmland load in Anhui Province increased from south to north. Fuyang and Suzhou cities had a high risk of water environment pollution and should be the key regions for livestock and poultry pollution prevention and control measures. The optimization of the layout of livestock and poultry farming areas, smoothing the cycles of crop and livestock farming, and the vigorous promotion of the resource utilization of the LPE are proposed.

Plant extraction is a thorough remediation method to remove heavy metals from soil. Chelating agents can enhance the extraction effect of heavy metals from soil by plants. In this study, low molecular weight organic acids (LMWOAs), such as citric acid (CA), tartaric acid (TA) and polyaspartate (PASP), were added to the farmland soil contaminated with Cd and Pb and combined with corn to enhance the absorption and extraction of Cadmium (Cd) and Plumbum (Pb). The effects of LMWOAs on the growth and yield of maize, Cd and Pb concentrations in each part of maize, rhizosphere soil pH, total cadmium and lead content and DTPA-Cd and Pb concentrations were studied. The enrichment, transport and extraction ability of maize were analyzed, and calcium chloride (CaCl₂) was compared. The results showed that: (1) Exogenous LMWOAs increased the biomass and yield of maize compared with no material added (CK), and CA increased the yield of maize by 6.33%. (2) Exogenous LMWOAs promoted the uptake of Cd and Pb in all parts of maize, and the distribution of Cd and Pb in all organs was as follows: straws > roots > maize cobs > kernels and roots > straws > maize cobs > kernels. The Cd and Pb concentrations in maize kernels were in line with GB 13078-2017 in China, which could be used as feed maize. (3) Exogenous LMWOAs enhanced the enrichment of Cd and Pb in maize straws and roots. PASP had a good enrichment effect on Cd and Pb. CA and CaCl₂ could enhance the transport capacity of Cd and Pb from maize roots to straw. (4) Compared with CK, the total amount of Cd and Pb in rhizosphere soil decreased by 6.93–26.99% and 2.74–6.79%, respectively. LMWOAs could promote the concentration of DTPA-Cd and Pb in rhizosphere soil, and PASP had the most significant increase in DTPA-Cd and Pb in rhizosphere soil. Compared with CK, the soil pH value decreased by 0.03–0.14 units. LMWOAs can enhance the removal of heavy metals from maize, and PASP has the most significant comprehensive effect.

The accumulation of heavy metals in rice is bound to affect human health and safety. In order to ensure food security, this study explores the effect of leaf surface regulation of zinc (Zn) fertilizer on the safety of rice in cadmium (Cd)-plumbum (Pb) polluted farmland. Through field experiments, the leaf surface control treatment of zinc fertilizer was set up, and the effects of leaf surface control of zinc fertilizer on rice yield, Cd and Pb concentration in different parts and zinc, nitrogen, phosphorus and potassium concentration in brown rice were studied in the growing period of rice. The results showed as follows: (1) Spraying twice or more in the growth stage of rice could increase the yield by 6.77–7.29% compared with the blank, which was significantly higher than that of single spraying in a certain growth stage. (2) After spraying zinc fertilizer on the leaf surface, Cd and Pb concentration in brown rice decreased by 29.52–56.01% and 11.10–28.34%, respectively, compared with CK. Two or more times of spraying can make Cd concentration in brown rice meet the Chinese standard GB 2762-2022, and one time of spraying can make Pb concentration in brown rice meet the standard. (3) Leaf surface control of zinc fertilizer could reduce the Cd enrichment ability of rice plant parts, and the Cd enrichment coefficient of brown rice was significantly reduced by 28.18–55.02%. Leaf surface control of zinc fertilizer can reduce Cd and Pb concentration in brown rice by reducing the transport ability of heavy metal Cd and Pb in rice roots to straw and then to brown rice. (4) The zinc concentration in brown rice was 18.16–20.68 mg·kg⁻¹, which was 18.21–34.64% higher than that in CK, and the zinc enrichment effect was the most significant after spraying three times. Meanwhile, the nitrogen, phosphorus and potassium concentration in brown rice also increased with the increase of spraying times. By comprehensive analysis, the leaf surface control of zinc fertilizer can reduce the Cd and Pb concentration in rice, and the Cd and Pb concentration in brown rice can meet the Chinese standard GB 2762-2022 by spraying twice. At the same time, it can improve the concentration of zinc, nitrogen, phosphorus and potassium in brown rice, is feasible and has high economic benefits.

We studied the influence and correlation of soil improvement, farmland ecological protection, soil fertilization, and field infrastructure construction on the quality grade of well-fertilized farmland in the engineering measures of well-fertilized farmland construction. Taking Xiao County of the Anhui Province as the study area, based on the software platforms of SPSS, ArcGIS10.6, and the county farmland resource management information system, we investigated the farmland quality changes of well-facilitated farmland before and after construction using the fuzzy evaluation method and analytic hierarchy process. We used principal component analysis and the gray relational method to analyze the impact and correlation of various engineering measures on farmland quality. The farmland quality grade in the study area was improved by a 0.59 grade after the construction of the well-facilitated farmland. Well-facilitated farmland construction engineering measures mainly affected the farmland quality through 12 factors, such as the soil bulk density, tillage layer texture, irrigation and drainage guarantee rates, forest network density, and field road accessibility. There is a strong correlation between these factors and the characteristics of farmland quality; the degrees of correlation were 0.865–0.610, respectively. The highest correlation degree was 0.939 between the deep plowing and deep loosening soil improvement project and the improvement of the well-facilitated basic farmland quality; this was followed by soil fertilization with an increased application of organic fertilizer, farmland ecological protection, and the field infrastructure project with correlations of 0.936, 0.857, and 0.563, respectively. Represented by the improvement of farmland fertility, the soil improvement project had the strongest impact on well-facilitated farmland quality. The soil fertility project, farmland ecological protection project, and the field infrastructure project were the second most important, with very close degrees of correlation.

Effective, economical and feasible remediation technology of heavy metal pollution in farmland is an important research concentration in the field of farmland soil remediation. In order to investigate the remediation effects of formula fertilizer and biochar on cadmium (Cd)–plumbum (Pb) contaminated farmland, blank (CK), lime (SH), biochar (SWT), formula fertilizer (FL), and biochar + formula fertilizer (FS) were set up in Cd–Pb contaminated farmland. The results demonstrated the following: (1) Compared with CK, SWT and FS increased the yield by 11.21% and 15.00%, respectively, which was significantly higher than other treatments. (2) The concentrations of Cd and Pb in maize kernels under all treatments were lower than the limited value stipulated by GB 2762-2022 in China. Compared with CK, FS reduced the concentration of Cd and Pb in maize kernels by 24.96% and 31.46%, respectively, which were the most significant. All the treatments can reduce the concentrations of Cd and Pb in maize cob and straw and inhibit the transfer of Cd and Pb from the lower part of the maize field to the overground part. (3) FL, SWT, and FS increased soil pH by 0.17, 0.10, and 0.19 units, respectively. FS can reduce the concentrations of available cadmium (DTPA-Cd) and available lead (DTPA-Pb) significantly, which are 31.05% and 38.57% lower than CK, respectively. (4) Each treatment can reduce the extraction state and reducible state of weak acid Cd and Pb in soil, while increasing the proportion of oxidizable state and residual state. FL and FS significantly increased the percentage of residual Cd and Pb by 18.00% and 24.32%, respectively, and 33.33% and 37.76%, respectively. (5) FL (1.747) and FS (1.679) were relatively higher than CK in input/output. In conclusion, the combined application of biochar and formula fertilizer in Cd/Pb polluted farmland can effectively reduce the concentration of Cd and Pb in maize and has high economic benefits and practicability.