The study was used in the context of realigning novel low-cost materials for their better and improved optical properties. Emphasis was placed on the bio-nanocomposite approach for producing cellulose/starch/silver nanoparticle films. These polymeric films were produced using the solution casting technique followed by the thermal evaporation process. The structural model of the bio-composite films (CS:CL-CNC7:3–50%) was developed from our previous study. Subsequently, in order to improve the optical properties of bio-composite films, bio-nanocomposites were prepared by incorporating silver nanoparticles (AgNPs) ex situ at various concentrations (5–50% w/w). Characterization was conducted using UV-Visible (UV-Vis), Fourier Transform Infrared (FTIR), Scanning Electron Microscope (SEM) and Transmission Electron Microscope (TEM) to understand the structure–property relationships. The FTIR analysis indicated a reduction in the number of waves associated with the OH functional groups by adding AgNPs due to the formation of new hydrogen bonds between the bio-composite matrix and the CL-WE-AgNPs. Based on mathematical equations, the optical bandgap energy, the energy of Urbach, the edge of absorption (Ed), and the carbon clusters (N) were estimated for CS:CL-CNC and CS:CL-CNC-AgNPs (5–50%) nanocomposite films. Furthermore, the optical bandgap values were shifted to the lower photon energy from 3.12 to 2.58 eV by increasing the AgNPs content, which indicates the semi-conductor effect on the composite system. The decrease in Urbach’s energy is the result of a decrease in the disorder of the biopolymer matrix and/or attributed to an increase in crystalline size. In addition, the cluster carbon number increased from 121.56 to 177.75, respectively, from bio-composite to bio-nanocomposite with 50% AgNPs. This is due to the presence of a strong H-binding interaction between the bio-composite matrix and the AgNPs molecules. The results revealed that the incorporation of 20% AgNPs into the CS:CL-CNC7:3–50% bio-composite film could be the best candidate composition for all optical properties. It can be used for potential applications in the area of food packaging as well as successfully on opto-electronic devices.

Ulva lactuca has been used to remove many toxic substances from industrial wastewater. In the present study we tried to optimize the efficiency of U. lactuca as an adsorbent of methylene blue (MB) in aqueous solution. U. lactuca was chemically treated with sulfuric acid (UL-H) and sodium hydroxide (UL-OH) and by a slow pyrolysis process (carbonization process) at high temperature T = 600 °C (UL-T) and compared to the nonactive Ulva (UL-NA) and the water insoluble substance (UL-WIS). Several spectroscopic analyses were carried out to detect the biosorption mechanisms of Ulva to remove MB in solution. The effects of different parameters on the adsorption process were studied, i.e., pH (2–10), mass concentration (1–10 g L⁻¹), and contact time (0–120 min). The results showed that the best adsorption of MB by Ulva was at pH = 8, with 5 g L⁻¹ of biomass at 75 min; the best adsorption capacity was 625.0 mg g⁻¹ for UL-OH, which was able to remove more than 89% of MB compared to UL-T, whose removal rate did not exceed 5%. Fourier-transform infrared spectroscopy (FTIR), energy-dispersive X-ray spectroscopy (EDX), and scanning electron microscopy (SEM) indicated the presence of oxygenated functional groups with a highly porous surface. The kinetic studies confirmed that the majority of treatments follow the pseudo-second-order type. The mathematical models showed that Langmuir model is favorable to UL-OH, UL-WIS, and UL-NA. According to the experimental results, the primary treatment for U. lactuca is a promising environmentally friendly method and an economical strategy for removing MB from aqueous solution. This method can help address the growing demand for adsorbents used in environmental protection processes and the resultant increase in their price.

In recent years, macroalgae and microalgae have played a significant role in the production of organic matter, fiber, and minerals on Earth. They contribute to both technical and medicinal applications as well as being a healthy and nutritious food for humans and animals. The theme of this work concerns the development and exploitation of Chaetomorpha linum (C. linum) biomass, through the elaboration of a new starch-based composite film reinforced by cellulose nanocrystals (CL-CNC) derived from C. linum. The first step involves the chemical extraction of CL-CNC from dry C. linum algae biomass. To achieve this, three types of cyclic treatment were adopted: alkalinization (sodium hydroxide) followed by bleaching (sodium hypochlorite) and acid hydrolysis (hydrochloric acid). We then studied the optimization of the development of bio-composite films based on corn starch (CS) reinforced by CL-CNC. These polymeric films were produced using the solution-casting technique followed by the thermal evaporation process. Structure and interactions were modified by using different amounts of glycerol plasticizers (20% and 50%) and different CS:CNC ratios (7:3 and 8:2). These materials were characterized by UV visible (UV/Vis), Fourier Transform Infrared (FTIR) and Scanning Electron Microscope (SEM) spectroscopy to understand structure-property relationships. The result revealed that the best matrix composition is 7:3 (CS: CL-CNC) with 50% glycerol, which reflects that the reinforcing effect of CL-CNC was greater in bio-composites prepared with a 50% plasticizer, revealing the formation of hydrogen bonds between CL-CNC and CS.

Bacterial secondary metabolites are a valuable source of various molecules that have antibacterial and anticancer activity. In this study, ten endosymbiotic bacteria of aphids, aphid predators and ants were isolated. Bacterial strains were identified according to the 16S rRNA gene. Ethyl acetate fractions of methanol extract (EA-ME) were prepared from each isolated bacterium and tested for their antibacterial activities using the disk diffusion method. The EA-ME of three bacterial species, Planococcus sp., Klebsiella aerogenes, Enterococcus avium, from the pomegranate aphids Aphis punicae, Chrysoperia carnea, and Tapinoma magnum, respectively, exhibited elevated antibacterial activity against one or several of the five pathogenic bacteria tested. The inhibition zones ranged from 10.00 ± 0.13 to 20.00 ± 1.11 mm, with minimum inhibitory concentration (MIC) values ranging from 0.156 mg/mL to 1.25 mg/mL. The most notable antibacterial activity was found in the EA-ME of K. aerogenes against Klebsiella pneumonia and Escherichia coli, with an MIC value of 0.156 mg/mL. The cytotoxic activity of EA-ME was dependent on the cell line tested. The most significant cytotoxicity effect was observed for extracts of K. aerogenes and E. avium, at 12.5 µg/mL, against the epithelial cells of lung carcinoma (A549), with a cell reduction of 79.4% and 67.2%, respectively. For the EA-ME of K. aerogenes and Pantoea agglomerans at 12.5 µg/mL, 69.4% and 67.8% cell reduction were observed against human colon cancer (Hct116), respectively. Gas chromatography–mass spectrometry (GC-MS) analysis of three EA-ME revealed the presence of several bioactive secondary metabolites that have been reported previously to possess antibacterial and anticancer properties. To the best of our knowledge, this is the first study to examine the biological activities of endosymbiotic bacteria in aphids, aphid predators and ants. The promising data presented in this study may pave the way for alternative drugs to overcome the continued emergence of multidrug-resistant bacteria, and find alternative drugs to conventional cancer therapies.

Aphids are a ubiquitous group of pests in agriculture that cause serious losses. For sustainable aphid identification, it is necessary to develop a precise and fast aphid identification tool. A new simple chemotaxonomy approach to rapidly identify aphids was implemented. The method was calibrated in comparison to the established phylogenetic analysis. For chemotaxonomic analysis, aphids were crushed, their headspace compounds were collected through closed-loop stripping (CLS) and analysed using gas chromatography—mass spectrometry (GC-MS). GC-MS data were then subjected to a discriminant analysis using CAP12.exe software, which identified key biomarkers that distinguish aphid species. A dichotomous key taking into account the presence and absence of a set of species-specific biomarkers was derived from the discriminant analysis which enabled rapid and reliable identification of aphid species. As the method overcomes the limits of morphological identification, it works with aphids at all life stages and in both genders. Thus, our method enables entomologists to assign aphids to growth stages and identify the life history of the investigated aphids, i.e., the food plant(s) they fed on. Our experiments clearly showed that the method could be used as a software to automatically identify aphids.

Introduction: The escalating threat posed by Aphis punicae to Punica granatum cultivation underscores the urgent need for sustainable, ecologically sound alternatives to chemical pesticides. This study employs a non-targeted analysis of volatile organic compounds (VOCs) emitted by aphid-infested pomegranate (AIP), undergoing multitrophic interactions with natural enemies (Coccinella undecimpunctata) and mutualistic protectors (Tapinoma magnum). These VOCs are hypothesized to function as early biochemical markers of pest stress and semiochemical cues guiding insect behavior, offering potential integration into decision-support tools within integrated pest management (IPM) frameworks.Methods: VOCs were non-destructively collected using open-loop stripping and analyzed via gas chromatography–mass spectrometry under a metabolomics approach. Profiling was conducted across four ecological scenarios through integrated in-situ experimentation: (G1) AIP, (G2) AIP with ants, (G3) AIP with ants and ladybirds (24h), and (G4) AIP with ants and ladybirds (48h). Principal component analysis and heatmap clustering revealed scenario-specific VOC fingerprints.Results: In the two-trophic AIP system, early plant stress responses included suppressed emissions of β-farnesene and methyl salicylate, alongside elevated levels of caryophyllene, a compound often associated with herbivore activity. At 24h, under a tritrophic interaction, 4-heptanone, a key ant pheromone, was detected, suggesting a role in interspecies signaling or predator deterrence. After 48h, in the quadripartite trophic interaction, VOCs such as 1-ethyl-3-methylbenzene, 1,3,5-trimethylbenzene, and 1-methyl-1H-imidazole became dominant, likely reflecting aphid-induced signaling affecting multitrophic dynamics. In the same interaction, elevated levels of six herbivore-induced plant volatiles (6-HIPVs), methyl salicylate, β-caryophyllene, sabinene, limonene, pentadecane, and heptadecane, were observed, supporting indirect plant defense by attracting natural enemies. Bioassays showed that C. undecimpunctata exhibited significantly higher attraction to the mixture of 6-HIPVs compared to individual treatments with methyl salicylate or β-caryophyllene. The mixture elicited the highest behavioral response, indicating a synergistic effect among volatiles and supporting their role in enhancing predator attraction.Discussion: To transition from discovery to application, future research should focus on targeted analysis, compound-specific bioassays, optimized delivery systems, and open-field trials. Assessing these VOCs under varying agroecological conditions, along with evaluating economic feasibility, scalability, and regulatory pathways. This approach will be crucial for translating this chemical ecology framework into effective, climate-resilient IPM strategies tailored to the arid agroecosystems of the Taif and similar environments.