Radio Frequency Identification (RFID) technology has established itself as an effective tool for identifying various objects in all human and business areas. There are many studies describing the use of this technology. However, scientific articles only marginally address the issue of recycling or reusing radio frequency identifiers. Radio frequency identifiers are defined as electronic waste by European Union legislation. This article deals with the environmental burden resulting from the use of radio frequency identifiers in a selected logistics centre and courier company in the Slovak and Czech Republic territories. The research and its relevance have become topical in the context of pandemics and with the increasing demand for products and courier services. In order to access the level of the above-mentioned environmental burden in a relevant way, an analysis of the circulation of transport units (pallets) and radio frequency identifiers in the selected logistics centre was carried out. The research results showed that the selected logistics centre generated annually 5.7 t of the e-waste from radio frequency identifiers placed on received pallets. The amount of 139 kg of metal was present in the e-waste quantity. The partial results of the research were applied to the e-commerce area. This article’s conclusion is dedicated to the characterization of possibilities of reducing the environmental burden from the use of radio frequency identifiers in logistics.