Little is known about the direct effects of microplastics (MPs) and their organic additives on marine bacteria, considering their role in the nutrient cycles, e.g., N-cycles through the N₂-fixation, or in the microbial food web. To fill this gap of knowledge, we exposed marine bacteria, specifically diazotrophs, to pure MPs which differ in physical properties (e.g., density, hydrophobicity, and/or size), namely, polyethylene, polypropylene, polyvinyl chloride and polystyrene, and to their most abundant associated organic additives (e.g., fluoranthene, 1,2,5,6,9,10-hexabromocyclododecane and dioctyl-phthalate). Growth, protein overproduction, direct physical interactions between MPs and bacteria, phosphorus acquisition mechanisms and/or N₂-fixation rates were evaluated. Cyanobacteria were positively affected by environmental and high concentrations of MPs, as opposed to heterotrophic strains, that were only positively affected with high concentrations of ~120 μm-size MPs (detecting the overproduction of proteins related to plastic degradation and C-transport), and negatively affected by 1 μm-size PS beads. Generally, the organic additives had a deleterious effect in both autotrophic and heterotrophic bacteria and the magnitude of the effect is suggested to be dependent on bacterial size. Our results show species-specific responses of the autotrophic and heterotrophic bacteria tested and the responses (beneficial: the “good,” deleterious: the “bad” and/or both: the “double-sword”) were dependent on the type and concentration of MPs and additives. This suggests the need to determine the threshold levels of MPs and additives concentrations starting from which significant effects can be observed for key microbial populations in marine systems, and these data are necessary for effective environmental quality control management.

The number of Vibrio -related infections in humans, e.g., by Vibrio vulnificus , has increased along the coasts of the Baltic Sea. Due to climate change, vibriosis risk is expected to increase. It is, therefore, pertinent to design a strategy for mitigation of the vibriosis threat in the Baltic Sea area, but a prerequisite is to identify the environmental conditions promoting the occurrence of pathogenic Vibrio spp., like V. vulnificus . To address this, we sampled three coastal Baltic sites in Finland, Germany, and Denmark with salinities between 6 and 21 from May to October 2022. The absolute and relative abundances of Vibrio spp. and V. vulnificus in water were compared to environmental conditions, including the presence of the eelgrass Zostera marina , which has been suggested to reduce pathogenic Vibrio species abundance. In the water column, V. vulnificus only occurred at the German station between July and August at salinity 8.1–11.2. Temperature and phosphate (PO ₄ ^3– ) were identified as the most influencing factors for Vibrio spp. and V. vulnificus . The accumulation of Vibrio spp. in the sediment and the co-occurrence with sediment bacteria in the water column indicate that sediment resuspension contributed to V. vulnificus abundance. Interestingly, V. vulnificus co-occurred with specific cyanobacteria taxa, as well as specific bacteria associated with cyanobacteria. Although we found no reduction in Vibrio spp. or V. vulnificus associated with eelgrass beds, our study underscores the importance of extended heatwaves and sediment resuspension, which may elevate the availability of PO ₄ ^3– , for Vibrio species levels at intermediate salinities in the Baltic Sea. IMPORTANCE: Elevated sea surface temperatures are increasing the prevalence of pathogenic Vibrio at higher latitudes. The recent increase in Vibrio -related wound infections and deaths along the Baltic coasts is, therefore, of serious health concern. We used culture-independent data generated from three Baltic coastal sites in Denmark, Germany, and Finland from May to October (2022), with a special focus on Vibrio vulnificus , and combined it with environmental data. Our temporal model shows that temperature, combined with sediment resuspension, drives the prevalence of V. vulnificus at intermediate salinities in the coastal Baltic Sea.

Non-cyanobacterial diazotrophs (NCDs) are widespread and active in marine waters. The carbon and low-oxygen (O ₂ ) conditions required for their N ₂ fixation may be encountered on marine particles, while a putative role of light remains uninvestigated. This study explored factors that regulate N ₂ fixation in Rhodopseudomonas sp. BAL398—a anoxygenic phototrophic bacterium isolated from low-salinity surface waters. Light (250 µmol photons m ⁻² s ⁻¹ ) and anoxia (0 µM O ₂ ) stimulated growth and N ₂ fixation; however, diazotrophy in light was dependent on high organic carbon levels (35 mM, glucose:succinate). Immunolabeling revealed that cellular nitrogenase levels increased with light, decreasing inorganic nitrogen (N) and ambient O ₂ (250 µM). Light and O ₂ stimulated motility and biofilm formation on surfaces, and N ₂ fixation rates increased compared to the control treatment. N ₂ fixation rates were positively correlated with the formation of rosette-like cellular structures, and an increased concentration of nitrogenase was observed toward the center of these structures, which increased their occurrence 600 times when cultures reached maximum N ₂ fixation rates vs when they had low rates. Interestingly, N ₂ fixation was not completely inhibited under oxic conditions and was accompanied by increased formation of capsules and cysts. Rosettes, as well as capsules and cysts, may thus serve as protection against O ₂ . Our study reveals the physiological adaptations that underlie N ₂ fixation in an anoxygenic phototroph, emphasizing the significance of biofilm formation for utilizing light and fixing N ₂ under oxic conditions, and underscores the need for deciphering the importance of light for marine NCDs. IMPORTANCE: The contribution of non-cyanobacterial diazotrophs (NCDs) to total N ₂ fixation in the marine water column is unknown, but their importance is likely constrained by the limited availability of dissolved organic matter and low O ₂ conditions. Light could support N ₂ fixation and growth by NCDs, yet no examples from bacterioplankton exist. In this study, we show that the phototrophic NCD, Rhodopseudomonas sp. BAL398, which is a member of the diazotrophic community in the surface waters of the Baltic Sea, can utilize light. Our study highlights the significance of biofilm formation for utilizing light and fixing N ₂ under oxic conditions and the role of cell plasticity in regulating these processes. Our findings have implications for the general understanding of the ecology and importance of NCDs in marine waters.

Two Legionella -like strains isolated from hot water distribution systems in 2012 have been characterized phenotypically, biochemically and genomically in terms of DNA relatedness. Both strains, HCPI-6^T and EUR-108, exhibited biochemical phenotypic profiles typical of Legionella species. Cells were Gram-negative motile rods which grew on BCYEα agar but not on blood agar and displayed phenotypic characteristics typical of the family Legionellaceae , including a requirement for l-cysteine and testing catalase positive. Both strains were negative for oxidase, urease, nitrate reduction and hippurate negative, and non-fermentative. The major ubiquinone was Q12 (59.4 % HCPI-6^T) and the dominant fatty acids were C_16 : 1 ω7c (28.4 % HCPI-6^T, ≈16 % EUR-108), C_16 : 0 iso (≈22.5 % and ≈13 %) and C_15 : 0 anteiso (19.5 % and ≈23.5 %, respectively). The percent G+C content of genomic DNA was determined to be 39.3 mol %. The 16S rRNA gene, mip sequence and comparative genome sequence-based analyses (average nucleotide identity, ANI; digital DNA–DNA hybridization, dDDH; and phylogenomic treeing) demonstrated that the strains represent a new species of the genus Legionella . The analysis based on the 16S rRNA gene sequences showed that the sequence similarities for both strains ranged from 98.8–90.1 % to other members of the genus. The core genome-based phylogenomic tree (protein-concatemer tree based on concatenation of 418 proteins present in single copy) revealed that these two strains clearly form a separate cluster within the genus Legionella . ANI and dDDH values confirmed the distinctiveness of the strains. Based on the genomic, genotypic and phenotypic findings from a polyphasic study, the isolates are considered to represent a single novel species, for which the name Legionella maioricensis sp. nov. is proposed. The type strain is HCPI-6^T (=CCUG 75071^T=CECT 30569^T).

The Balearic Islands, a top tourist destination for sunny beaches, face physical and chemical pressures from human activities, impacting keystone species like the endemic seagrass Posidonia oceanica and its associated microbiome. This study evaluated the effects of ZnO and TiO₂ nanoparticles and three commercial sunscreens with varying protection factors (50 or 90) and chemical complexities (1- SPF50_E "eco-friendly"; 2- SPF50 not "eco-friendly"; 3- SPF90 not "eco-friendly") on five heterotrophic bacteria (Pseudomonas azotifigens, Marinobacterium litorale, Thiothrix nivea, Sedimenticola thiotaurini and Cobetia sp) and two autotrophic cyanobacteria (Halothece sp. and Fischerella muscicola) associated to P. oceanica, as well as a natural leaf epiphytic community. Results indicated that TiO₂ affected all heterotrophic bacteria, while ZnO was toxic to only two species, while autotrophs were unaffected. Commercial sunscreens impacted three heterotrophs and the natural epiphytic community, while autotrophs were only affected by SPF50. SPF50_E reduced phosphorus uptake, and both SPF50 and SPF90 decreased alkaline phosphatase activity. Reactive oxygen species production was mainly induced by SPF90, followed by SPF50_E and SPF50. Generally, the smallest bacteria were most sensitive to UV-filters (UVFs). This study indicates that UVFs exposure may alter the epiphytic community structure of P. oceanica.