Background and Aim: Anaplasma platys is a dog pathogen that causes anaplasmosis in various hosts, including humans. It is a rickettsial pathogen that causes cyclic thrombocytopenia in primary canine recipients and is spread by the brown dog tick Rhipicephalus sanguineus. This study aimed to map the genetic sequences of Anaplasma spp. isolates comparable with those of different global locations and determine the infection status of Pshdar Kurdish shepherd dogs from three regions in Sulaymaniyah province who did not exhibit clinical indications for Anaplasma. Materials and Methods: A total of 75 dog blood samples were collected from the center of the Sulaymaniyah, Dukan, and Ranya districts in the Sulaymaniyah province and subjected to polymerase chain reaction to determine the 16S ribosomal RNA (16S rRNA) gene of A. platys. Results: Only two dogs (2.7%) were positive for A. platys. The 16S rRNA gene of A. platys was sequenced and registered in the National Center for Biotechnology Information GenBank with accession number OR467538. With four nucleotide changes, the sequence exhibited 99.72% similarity to strains identified as human infections and those found in recognized tick vectors. Conclusion: We conclude that the blood of Pshdar Kurdish shepherd dogs in the Sulaymaniyah region of Iraq contains a small number of A. platys. Moreover, the phylogenetic tree of the isolated species, A. platys, was significantly similar to other strains of A. platys found worldwide. In the Kurdistan region of Iraq, this study represents the first molecular detection of the 16S rRNA gene of A. platys. Keywords: 16S ribosomal RNA gene, Anaplasma platys, blood, dogs, polymerase chain reaction.

The Studies of genetic polymorphism in local animals aim at evaluating the genetic diversity of animal breeds chiefly for conservation purposes. In this research, single nucleotide polymorphism (SNP) was used to detect point mutation of GH, IGF1, BMP15 genes in Maraz goat. Forty blood samples were collected from Maraz goat, DNA was isolated and primer was used to amplify GH, IGF1, and BMP15 genes. Finally, sequencing methodology was applied for PCR products. Results of GH gene showed only one SNPs (AG) at nucleotide number 58pb. The sequence results of IGF1 illustrated two point mutations at nucleotide number 5502 and 5675 bp. While according to data sequences no point mutation was detected of BMP15 gene. These outcomes revealed that the SNP marker of the GH, IGF-I, and BMP15 genes will be a possible molecular marker for growth traits and cashmere fiber in Maraz goat. This research displays that the genetic similarities between Maraz goat and other breeds match their geographical distribution which indicates that the Maraz goat is robustly separated from the other breeds.