Despite the efforts to improve the methodological standards of silage trials, many factors that can influence the results of lab-scale studies need to be better understood. This study aimed to determine the effects of short-time delayed sealing and inoculation with a blend of Lentilactobacillus buchneri and Lactiplantibacillus plantarum on fermentation, aerobic stability, and chemical composition of silages. Whole-crop maize was treated with or without a commercial inoculant and ensiled (29.3% dry matter) for 55 days in 8.8 L PVC silos that were sealed immediately (up to 30 min delay) or after a delay (90, 150, or 210 min between chopping and sealing) with five replicates each. The increasing air exposure before sealing increased fermentation losses and reduced silage nutritional value. Crude protein and ash were significantly affected by inoculation, with control treatments showing higher ash and lower protein values. Lignin, neutral detergent fiber, and acid detergent fiber were only affected by the delay period. The longer the sealing delay, the higher the gas production, and the lower the starch values and lactic acid content observed in samples. Inoculation was inefficient in reducing total dry matter losses, but it increased aerobic stability, acetic acid, and ethanol contents of silages and reduced effluent loss. Control silages had higher total dry matter loss during the aerobic exposure than inoculated silages. The results confirmed that the delay periods tested were long enough to negatively interfere with the chemical composition of silages, especially the fibrous fraction content.

This study aimed to evaluate the effects of two maturity stages of whole-plant corn at harvest (32.1 or 42.5% dry matter (DM)) and a commercial microbial inoculant composed of L. buchneri and L. plantarum at concentration of 110,000 CFU/g of fresh forage (1.1 × 105 CFU/g FF) on fermentation losses, aerobic stability, chemical composition, and digestibility of starch and neutral detergent fiber (NDF). A factorial and randomized design was used (two DM contents, both with or without inoculant), with [...] ABSTRACT This study aimed to evaluate the effects of two maturity stages of whole-plant corn at harvest (32.1 or 42.5% dry matter (DM)) and a commercial microbial inoculant composed of L. buchneri and L. plantarum at concentration of 110,000 CFU/g of fresh forage (1.1 × 105 CFU/g FF) on fermentation losses, aerobic stability, chemical composition, and digestibility of starch and neutral detergent fiber (NDF). A factorial and randomized design was used (two DM contents, both with or without inoculant), with