Social media represents a powerful tool for disseminating verified health information on topics such as colostomy, and the roles of healthcare professionals and institutions to ensure the veracity of the information conveyed is increasingly relevant. The main objectives of this study were to analyze the roles of these healthcare professionals and institutions in the conversation about colostomy, without being framed in a specific health communication campaign, and to know the use of reliable information in the conversation. The study was carried out by analyzing Twitter messages containing the hashtag “colostomy” and “Chron” between the 1 January and the 30 April 2022. It was conducted using the NodeXL software, focusing on content analysis of tweets and users’ accounts. The results show that accounts with healthcare activity influence the impressions generated on the network (p = 0.018), finding that nurses are the most active healthcare professionals (22.24%) also having a significant effect on the overall network interactions (p = 0.022). In contrast, we found that institutions do not actively participate on the network. We emphasize the responsibility of institutions for health education and the need for professionals to improve communication skills on social networks, but also the need to improve communication skills on social media to support public health campaigns through these increasingly important channels.