A simple, rapid, precise, accurate and sustainable spectrofluorimetric method (SFM) was developed, validated and applied for the determination of 4-aminobenzoic acid and aromatic amino acids (phenylalanine, tryptophan and tyrosine). These compounds are used in biopharmaceutical formulations and therefore must be analyzed by quality control laboratories to meet the criteria established in pharmacopoeias. In general, potentiometric titration (PT) is described in the compendia as the official analytical technique. However, this method showed low sensitivity and selectivity, and moreover was performed with a non-aqueous solvent (acetic acid), which led to higher consumption of reagents and consequently to the formation of residues. Therefore, the SFM was developed in aqueous medium at pH 7.2 using phosphate buffer. It was successfully validated according to the ICH guidelines and showed good linearity range (r>0.999), specificity, accuracy and precision (within and between days) and robustness. The test results were compared between the SFM and PT using raw material samples, while according to the F- and t-tests at 95% confidence level, no statistical difference was found between the methods.

The reuse of contaminated water arising from industries is a major challenge in the environmental field, which enables the search for treatments with a view to sustainable development. In this context, the objective of this study was to evaluate the effect of the calcination material from an ant colony (termite) in the production of adsorbent materials for methylene blue dye removal. The natural material (NC) and calcined materials at temperatures of 290ºC (C-290), 380ºC (C-380), 450ºC (C-450) and 540ºC (C-540) were characterized using techniques of thermal analysis and Fourier Transform Infrared Spectroscopy (FTIR). In NC and C-290 samples, the volatile compounds were evaluated by HS-GC/MS and phenolic compounds were identified by HPLC. The results for the adsorption of the dye methylene blue showed that, while the adsorption process can not be quantified employing the natural material, the process efficiency was quite relevant when the sample was calcined at a temperature of 290°C, whose removal percentage was 96%. Although the calcined sample at this temperature is the most similar to the starting material, performed at a temperature of 290°C acted in reducing the complexity of phenolic and volatile compounds found in the NC sample, which may negatively impact in the adsorption process.

Purpose: Analytical methods of pharmaceutical quality control (QC) are typically selected for regulatory compliance and analytical performance, while sustainability and operational feasibility are often secondary. This case study aimed to demonstrate how combining greenness and whiteness metrics can support method-related decisions in QC under batch practice. Methods: Compendial non-aqueous potentiometric titrations (PT) for aromatic amino acids used as raw materials were compared with alternatives spectrofluorimetric methods (SFM) developed for assays. The Advanced National Environmental Methods Index (advanced NEMI) was applied during development as an early screening tool, followed by the Analytical Eco-Scale and Green Analytical Procedure Index (GAPI), and Analytical GREEnness metric (AGREE) to quantify and localize environmental burdens under finalized conditions. Finally, White Analytical Chemistry (WAC) was employed to integrate analytical performance, environmental impact, and implementation practicality. Results: Across these tools, PT was penalized by corrosive non-aqueous chemistry and higher hazardous reagent and waste demands, implying greater safety controls and waste-handling effort at scale. Triangulation converged on the same decision signal: SFM remained fit for release testing while reducing hazardous touchpoints and simplifying waste logistics, yielding superior greenness indicators (Eco-Scale: 93 vs. 76; AGREE: 0.61 vs. 0.34) and a higher WAC score (81.3 vs. 65.5). Conclusion: Overall, the metric set shifted the basis from “analytically compliant” to “fit for deployment,” framed by a Six Sigma improvement logic (Define–Measure–Analyze–Improve–Control), thereby strengthening sustainability-informed analytical development and change-control decisions in industrial QC.