The microalga Coelastrella rubescens dwells in habitats with excessive solar irradiation; consequently, it must accumulate diverse compounds to protect itself. We characterized the array of photoprotective compounds in C. rubescens. Toward this goal, we exposed the cells to high fluxes of visible light and UV-A and analyzed the ability of hydrophilic and hydrophobic extracts from the cells to absorb radiation. Potential light-screening compounds were profiled by thin layer chromatography and UPLC-MS. Coelastrella accumulated diverse carotenoids that absorbed visible light in the blue–green part of the spectrum and mycosporine-like amino acids (MAA) that absorbed the UV-A. It is the first report on the occurrence of MAA in Coelastrella. Two new MAA, named coelastrin A and coelastrin B, were identified. Transmission electron microscopy revealed the development of hydrophobic subcompartments under the high light and UV-A exposition. We also evaluate and discuss sporopollenin-like compounds in the cell wall and autophagy-like processes as the possible reason for the decrease in sunlight absorption by cells, in addition to inducible sunscreen accumulation. The results suggested that C. rubescens NAMSU R1 accumulates a broad range of valuable photoprotective compounds in response to UV-A and visible light irradiation, which indicates this strain as a potential producer for biotechnology.

Microalga Chlorella (Chromochloris) zofingiensis has been gaining increasing attention of investigators as a potential competitor to Haematococcus pluvialis for astaxanthin and other xanthophylls production. Phytohormones, including abscisic acid (ABA), at concentrations relevant to that in hydroponic wastewater, have proven themselves as strong inductors of microalgae biomass productivity and biosynthesis of valuable molecules. The main goal of this research was to evaluate the influence of phytohormone ABA on the physiology of C. zofingiensis in a non-aseptic batch experiment. Exogenous ABA stimulated C. zofingiensis cell division, biomass production, as well as chlorophyll, carotenoid, and lipid biosynthesis. The relationship between exogenous ABA concentration and the magnitude of the observed effects was non-linear, with the exception of cell growth and biomass production. Fatty acid accumulation and composition depended on the concentration of ABA tested. Exogenous ABA induced spectacular changes in the major components of the culture microbiome of C. zofingiensis. Thus, the abundance of the representatives of the genus Rhodococcus increased drastically with an increase in ABA concentration, whereas the abundance of the representatives of Reyranella and Bradyrhizobium genera declined. The possibilities of exogenous ABA applications for the enhancing of the biomass, carotenoid, and fatty acid productivity of the C. zofingiensis cultures are discussed.

Microalgae are naturally adapted to the fluctuating availability of phosphorus (P) to opportunistically uptake large amounts of inorganic phosphate (P_i) and safely store it in the cell as polyphosphate. Hence, many microalgal species are remarkably resilient to high concentrations of external P_i. Here, we report on an exception from this pattern comprised by a failure of the high P_i-resilience in strain Micractinium simplicissimum IPPAS C-2056 normally coping with very high P_i concentrations. This phenomenon occurred after the abrupt re-supplementation of P_i to the M. simplicissimum culture pre-starved of P. This was the case even if P_i was re-supplemented in a concentration far below the level toxic to the P-sufficient culture. We hypothesize that this effect can be mediated by a rapid formation of the potentially toxic short-chain polyphosphate following the mass influx of P_i into the P-starved cell. A possible reason for this is that the preceding P starvation impairs the capacity of the cell to convert the newly absorbed P_i into a “safe” storage form of long-chain polyphosphate. We believe that the findings of this study can help to avoid sudden culture crashes, and they are also of potential significance for the development of algae-based technologies for the efficient bioremoval of P from P-rich waste streams.

Modern methods of metagenomics, including those based on long reads of nanopore sequencing, are especially relevant for environmental-monitoring tasks, in particular, for assessing the anthropogenic impact on natural communities of microorganisms. These include algal-bacterial communities that live in water-treatment plants and participate in the biological treatment of wastewater from excess organic waste and nutrients (phosphorus and nitrogen). Metagenomic analysis shows changes in the taxonomic composition and functional profile of the algal-bacterial community when simulating the release of drugs into wastewater: broad-spectrum antibiotics (for example, ceftriaxone) and nonsteroidal anti-inflammatory drugs (for example, diclofenac). The results of metagenomic analysis based on long reads of nanopore sequencing and classical methods of environmental monitoring, including metabarcoding based on short reads on a second-generation sequencing platform, are compared. Nanopore sequencing of the entire metagenome shows a greater biodiversity of samples compared to the DNA metabarcoding of short reads, makes it possible to determine the taxonomic affiliation of particular organisms more accurately, identify groups of eukaryotic oxygenic phototrophs, and also reveal the presence of antibiotic-resistance genes in the community. It is shown that under the influence of medicinal substances, the algal-bacterial community is enriched with antibiotic-resistant bacteria. At the same time, despite maintaining the rate of phosphorus removal, the community’s potential for the removal of nutrients is reduced, which is expressed in a two-order reduction in the relative representation of inorganic-nutrient metabolism genes. Simultaneous exposure to ceftriaxone and excess nutrients in the wastewater leads to the maximum enrichment of antibiotic-resistant organisms, as well as the replacement of cyanobacteria originally present in the community with eukaryotic microalga.

Pharmaceuticals including antibiotics are among the hazardous micropollutants (HMP) of the environment. Incomplete degradation of the HMP leads to their persistence in water bodies causing a plethora of deleterious effects. Conventional wastewater treatment cannot remove HMP completely and a promising alternative comprises biotechnologies based on microalgae. The use of immobilized microalgae in environmental biotechnology is advantageous since immobilized cultures allow the recycling of the microalgal cells, support higher cell densities, and boost tolerance of microalgae to stresses including HMP. Here, we report on a comparative study of HMP (exemplified by the antibiotic ceftriaxone, CTA) removal by suspended and chitosan-immobilized cells of Lobosphaera sp. IPPAS C-2047 in flasks and in a column bioreactor. The removal of CTA added in the concentration of 20 mg/L was as high as 65% (in the flasks) or 85% (in the bioreactor). The adsorption on the carrier and abiotic oxidation were the main processes contributing 65–70% to the total CTA removal, while both suspended and immobilized cells took up 25–30% of CTA. Neither the immobilization nor CTA affected the accumulation of arachidonic acid (ARA) by Lobosphaera sp. during bioreactor tests but the subsequent nitrogen deprivation increased ARA accumulation 2.5 and 1.7 times in the suspended and chitosan-immobilized microalgae, respectively. The study of the Lobosphaera sp. microbiome revealed that the immobilization of chitosan rather than the CTA exposure was the main factor displacing the taxonomic composition of the microbiome. The possibility and limitations of the use of chitosan-immobilized Lobosphaera sp. IPPAS C-2047 for HMP removal coupled with the production of valuable long-chain polyunsaturated fatty acids is discussed.