Axonal transport of mitochondria is critical for neuronal survival and function. Automatically quantifying and analyzing mitochondrial movement in a large quantity remain challenging. Here, we report an efficient method for imaging and quantifying axonal mitochondrial transport using microfluidic-chamber-cultured neurons together with a newly developed analysis package named “MitoQuant”. This tool-kit consists of an automated program for tracking mitochondrial movement inside live neuronal axons and a transient-velocity analysis program for analyzing dynamic movement patterns of mitochondria. Using this method, we examined axonal mitochondrial movement both in cultured mammalian neurons and in motor neuron axons of Drosophila in vivo. In 3 different paradigms (temperature changes, drug treatment and genetic manipulation) that affect mitochondria, we have shown that this new method is highly efficient and sensitive for detecting changes in mitochondrial movement. The method significantly enhanced our ability to quantitatively analyze axonal mitochondrial movement and allowed us to detect dynamic changes in axonal mitochondrial transport that were not detected by traditional kymographic analyses.

Non-contact human body measurement plays an important role in surveillance, physical healthcare, on-line business and virtual fitting. Current methods for measuring the human body without physical contact usually cannot handle humans wearing clothes, which limits their applicability in public environments. In this paper, we propose an effective solution that can measure accurate parameters of the human body with large-scale motion from a Kinect sensor, assuming that the people are wearing clothes. Because motion can drive clothes attached to the human body loosely or tightly, we adopt a space-time analysis to mine the information across the posture variations. Using this information, we recover the human body, regardless of the effect of clothes, and measure the human body parameters accurately. Experimental results show that our system can perform more accurate parameter estimation on the human body than state-of-the-art methods.

Railway track detection, which is crucial for train operational safety, faces numerous challenges such as the curved track, obstacle occlusion, and vibrations during the train’s operation. Most existing methods for railway track detection use a camera or LiDAR. However, the vision-based approach lacks essential 3D environmental information about the train, while the LiDAR-based approach tends to detect tracks of insufficient length due to the inherent limitations of LiDAR. In this study, we propose a real-time method for railway track detection and 3D fitting based on camera and LiDAR fusion sensing. Semantic segmentation of the railway track in the image is performed, followed by inverse projection to obtain 3D information of the distant railway track. Then, 3D fitting is applied to the inverse projection of the railway track for track vectorization and LiDAR railway track point segmentation. The extrinsic parameters necessary for inverse projection are continuously optimized to ensure robustness against variations in extrinsic parameters during the train’s operation. Experimental results show that the proposed method achieves desirable accuracy for railway track detection and 3D fitting with acceptable computational efficiency, and outperforms existing approaches based on LiDAR, camera, and camera–LiDAR fusion. To the best of our knowledge, our approach represents the first successful attempt to fuse camera and LiDAR data for real-time railway track detection and 3D fitting.

Conventional deconvolution methods assume that the microscopy system is spatially invariant, introducing considerable errors. We developed a method to more precisely estimate space-variant point-spread functions from sparse measurements. To this end, a space-variant version of deblurring algorithm was developed and combined with a total-variation regularization. Validation with both simulation and real data showed that our PSF model is more accurate than the piecewise-invariant model and the blending model. Comparing with the orthogonal basis decomposition based PSF model, our proposed model also performed with a considerable improvement. We also evaluated the proposed deblurring algorithm. Our new deblurring algorithm showed a significantly better signal-to-noise ratio and higher image quality than those of the conventional space-invariant algorithm.