Toxoplasma gondii is a worldwide distributed protozoan parasite. This apicomplexan parasite infects one-third of the population worldwide, causing toxoplasmosis, considered one of the neglected parasitic infections. In healthy humans, most infections are asymptomatic. However, in immunocompromised patients, the course of the disease can be life-threatening. Human immunodeficiency virus (HIV)-infected patients have a very high burden of Toxoplasma gondii co-infection. Thus, it is essential to use modern, sensitive, and specific methods to properly monitor the course of toxoplasmosis in immunodeficient patients.

Cold-water immersion (CWI) after exercise is a method used by sportsmen to improve recovery. The aim of the study was to assess the effect of a 3 min CWI on the inflammatory state by measuring levels of interleukin 6 (IL-6), interleukin 10 (IL-10), tumor necrosis factor α (TNF-α), and transforming growth factor β1 (TGF-β1), and activities of α1-antitrypsin (AAT) and lysosomal enzymes, including arylsulfatase (ASA), acid phosphatase (AcP), and cathepsin D (CTS D), in the blood of healthy recreational athletes. Male volunteers (n = 22, age 25 ± 4.8 yr) performed a 30 min submaximal aerobic exercise, followed by a 20 min rest at room temperature (RT-REST) or a 20 min rest at room temperature with an initial 3 min 8 °C water bath (CWI-REST). Blood samples were taken at baseline, immediately after exercise, and after 20 min of recovery. The IL-6, IL-10, and TNF-α levels and the AAT activity increased significantly immediately after exercise. The IL-6 level was significantly higher after CWI-REST than after RT-REST. No changes in the activities of the lysosomal enzymes were observed. The effect of a 3 min CWI on the level of inflammatory markers during post-exercise recovery was limited. Thus, it might be considered as a widely available method of regeneration for recreational athletes.

Giardia intestinalis is one of the most common food-borne protozoa. The sensitivity of pathogens to physical and chemical factors is the basis for developing measures to reduce the incidence of the population. Several methods are available to detect the presence of G. intestinalis. The study determines the influence of 22 selected factors on the survival assessment and detection of G. intestinalis DNA in trophozoites axenically cultured. The influence of a given factor on the test result was observed in the case of 17 factors (77.3%) in the microscopic method, while only in the case of 3 (13.6%) substances in the real-time PCR method. Prevention of G. intestinalis infections, e.g., by ensuring food and water safety, is a crucial issue affecting public health. The experiment was conducted on trophozoites as the first approach. It is necessary to continue research and observe the epidemiological situation. In future studies, the impact of the studied factors on the survival assessment and detection of Giardia intestinalis DNA in axenically cultured cysts should be determined.

Background: Giardia intestinalis is one of the most common parasites in humans. Contaminated food and water can be a source of infection. Substances added to food are intended to increase its safety. We aimed to determination of the influence of various microorganisms and compounds that stimulate digestive functions, as well as preservatives and antioxidants on the detection of G. intestinalis by microscopic and immunoenzymatic methods. Methods: Twenty stool samples, archived in 1998-2018 in the Provincial Sanitary and Epidemiological Station in Bydgoszcz (Poland), collected both from patients referred for parasitic examinations by a doctor of a medical facility and from private individuals, were used to assess the impact of selected factors (such as bacterial strains, viruses and substances added to food) on the detection of G. intestinalis by microscopic and immunoenzymatic methods. Results: G. intestinalis was detected by both microscopic and immunoenzymatic methods with the same sensitivity (100%). The result of the G. intestinalis determination was positive in 90% of the samples after the addition of potassium sorbate, and in 25% of the samples after the addition of citric acid. Conclusion: The presence of other microorganism such as bacteria and viruses does not influence on the detection of G. intestinalis by microscopic and immunoenzymatic methods in stool samples. Citric acid as an antioxidant added to foods affects the detection of G. intestinalis. Due to the small number of samples used, it is necessary to continue research on the impact of various factors on the detection of protozoa.