Several studies have been carried out on the antimicrobial potential of natural flora, on various bacteria (Gram-negative and Gram-positive) and fungi. Among the pathogens of great importance are those of the genus Colletotrichum, as they cause anthracnose in a wide variety of plants. The aim of this work was to determine the antimicrobial activities (AMA) of plant extracts (PE) and essential oils (EO) of plant material collected in the northeastern region of Colombia. For EO obtained from Piper tenue Kunth, Piper eriopodon (Miq.) C. DC., Piper marginatum Jacq., Hyptis suaveolens (L.) Poit, Eriope crassipes Benth. and Lippia origanoides Kunth., were evaluated the occurrence of inhibition halos against Staphylococcus aureus, Bacillus cereus, Salmonella typhimurium, Klebsiella spp., Escherichia coli, Aspergillus terreus, Aspergillus fumigatus, Aspergillus niger, Rhizopus spp. and Candida spp. On the other hand, the growth inhibition of Colletotrichum musae Berk. & Curt. and Colletotrichum asianum was observed for the PE obtained from Ocotea aff. Ocotea caparrapi (Sand.-Groot ex Nates) Dugand, Trattinnickia rhoifolia Willd., Tetragastris panamensis (Engl.) Kuntze and Siparuna guianensis Aubl.. L. origanoides EO and E. crassipes EO, inhibited the growth of S. typhimurium, A. terreus and Candida spp. For C. musae: O. aff. O. caparrapi and T. rhoifolia (1.0% w/v), showed a percentage inhibition of mycelial growth (IMG), and a percentage reduction of conidial production (RCP), greater than 85%; T. panamensis and S. guianensis showed IMG and RCP greater than 71%. For C. asianum: O. aff. O. caparrapi (1.0% w/v), achieved inhibition greater than 83% (IMG and RCP), followed by S. guianensis, T. panamensis and T. rhoifolia. AMA showed a high correlation with phenolic content. The EO of L. origanoides and E. crassipes, and the PE of O. aff. O. caparrapi exhibited high AMA, comparable to that of the controls.