Key villages of rural tourism have become an important carrier for the high-quality development of rural tourism. The precise identification of the spatial distribution characteristics and influencing factors of rural tourism key villages is of great value in promoting the quality upgrading of rural tourism in China and realizing the goal of rural revitalization strategy. The aim is to realize the complementary coordination and integration of urban and rural areas, promote rural transformation and increase farmers’ incomes. Taking three batches of 1199 key rural tourism villages in China as research samples, the nearest neighbor index, disequilibrium index and kernel density methods were used to reveal the spatial differentiation characteristics of key rural tourism villages and the influencing factors were further analyzed using the geographical connection rate and buffer analysis. The results showed that (1) the key villages of rural tourism demonstrated a typical agglomeration in the spatial distribution, with the Hu Huanyong line as the obvious boundary. (2) The very high-density agglomeration centers were mainly located in the Beijing–Tianjin–Hebei region and the Yangtze River Delta region, while the high-density agglomeration zones were mainly located in the North China Plain on the east side of the Taihang Mountains and the middle and lower reaches of the Yangtze River Basin. (3) The spatial distribution characteristics of hot regions were in the central and eastern regions; however, the cold regions were in the northwest and northeast regions. (4) The spatial distribution characteristics of key rural tourism villages were the result of the interaction and coupling of multiple factors. The key villages of rural tourism were mainly distributed in plain and hilly areas with dense river networks, dense populations, high levels of economic development, developed transportation and suburban areas.

Industrial heritage is a valuable spatial resource for urban stock updates, and its preservation and reuse play an important role in the transmission of urban history and culture. Typological analysis, descriptive statistical analysis, relevant spatial analysis using ArcGIS 10.8, and geographic probes were employed to explore the spatial distribution characteristics and influencing factors regarding the current status of the reuse of 196 heritage sites selected from five batches of China’s National Industrial Heritage (NIH) lists. The results reveal the following: (1) The spatial distribution of China’s NIH sites is uneven and cohesive, forming a dense circle with the Yangtze River Delta region and the Beijing–Tianjin–Hebei region at its core. (2) Three-fourths of the NIH sites have entered the reuse stage, and high-density and relatively high-density clusters have formed in the eastern and central regions. (3) The conservation and reuse directions of China’s NIH sites are mainly divided into publicization and marketization. (4) The spatial distribution differentiation of the reuse of NIH sites is not due to a single cause but, rather, a combination of various contributing factors. Natural geographic and socio-economic factors exert varying degrees of influence on the spatial distribution of reused heritage sites, with tourism resources and government support playing primary roles in shaping this distribution. These findings establish a fundamental database of China’s NIH sites and provide guidance for the current conservation and reuse of industrial heritage.

Salix viminalis L., a dioecious species, is widely distributed in riparian zones, and flooding is one of the most common abiotic stresses that this species suffers. In this study, we investigated the morphological, anatomical, and physiological responses of male vs. female plants of S. viminalis to flooding. The results showed that the plant height and root collar diameter were stimulated by flooding treatment, which corresponded with higher dry weight of the stem and leaf. However, the dry weight of the underground part decreased, which might be due to the primary root having stopped growing. The little-influenced net photosynthesis rate (P_n) under flooding treatment could guarantee rapid growth of the aboveground part, while the unaffected leaf anatomical structure and photosynthetic pigment contents could ensure the normal operation of photosynthetic apparatus. Under a flooding environment, the production ratio of superoxide free radical (O₂^∙-) and malondialdehyde (MDA) contents increased, indicating that the cell membrane was damaged and oxidative stress was induced. At the same time, the antioxidant enzyme system, including superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX), and osmotic adjustment substances, involving proline (Pro) and solute protein (SP), began to play a positive role in resisting flooding stress. Different from our expectation, the male and female plants of S. viminalis performed similarly under flooding, and no significant differences were discovered. The results indicate that both male and female plants of S. viminalis are tolerant to flooding. Thus, both male and female plants of S. viminalis could be planted in frequent flooding zones.

Methyl-CpG-binding domain (MBD) proteins have diverse molecular and biological functions in plants. Most studies of MBD proteins in plants have focused on the model plant Arabidopsis thaliana L. Here we cloned SvMBD5 from the willow Salix viminalis L. by reverse transcription-polymerase chain reaction (RT-PCR) and analyzed the structure of SvMBD5 and its evolutionary relationships with proteins in other species. The coding sequence of SvMBD5 is 645 bp long, encoding a 214 amino acid protein with a methyl-CpG-binding domain. SvMBD5 belongs to the same subfamily as AtMBD5 and AtMBD6 from Arabidopsis. Subcellular localization analysis showed that SvMBD5 is only expressed in the nucleus. We transformed Arabidopsis plants with a 35S::SvMBD5 expression construct to examine SvMBD5 function. The Arabidopsis SvMBD5-expressing line flowered earlier than the wild type. In the transgenic plants, the expression of FLOWERING LOCUS T and CONSTANS significantly increased, while the expression of FLOWERING LOCUS C greatly decreased. In addition, heterologously expressing SvMBD5 in Arabidopsis significantly inhibited the establishment and maintenance of methylation of CHROMOMETHYLASE 3 and METHYLTRANSFERASE 1, as well as their expression, and significantly increased the expression of the demethylation-related genes REPRESSOR OF SILENCING1 and DEMETER-LIKE PROTEIN3. Our findings suggest that SvMBD5 participates in the flowering process by regulating the methylation levels of flowering genes, laying the foundation for further studying the role of SvMBD5 in regulating DNA demethylation.

Assessing and analyzing the spatial and temporal evolution of urban land use and habitat quality is important for the ecological transformation, habitat conservation, and ecological development of resource-depleted cities. The InVEST model and ArcGIS 10.5 were used to analyze the characteristics of land use transition, the temporal and spatial evolution of habitat quality, and the distribution of hot and cold spots in typical resource-depleted cities in central China from 1990 to 2020, with a view to providing a reference for ecological environmental protection of such cities. The results show the following: (1) During the study period, a decrease in cultivated land and forested land and an increase in construction land were the main reasons for the decline in habitat quality in the study area; thus, attention should be paid to protecting cultivated land and preventing forested land degradation. (2) In terms of the temporal gradient, the overall habitat quality in the region showed a declining trend, habitat degradation characteristics showed a spread from urban city and county areas as the core to the surrounding areas. (3) In terms of spatial distribution, habitat quality in the area shows a pattern of “high in the north and low in the middle”.