The total area under quinoa (Chenopodium quinoa Willd.) cultivation and the consumption of its grain have increased in recent years because of its nutritional properties and ability to grow under adverse conditions, such as drought. Climate change scenarios predict extended periods of drought and this has emphasized the need for new crops that are tolerant to these conditions. The main goal of this work was to evaluate crop yield and quality parameters and to characterize the physiology of two varieties of quinoa grown under water deficit in greenhouse conditions. Two varieties of quinoa from the Chilean coast (Rainbow) and altiplano (Illpa) were used, grown under full irrigation or two different levels of water deficit applied during the grain filling period. There were no marked differences in yield and quality parameters between treatments, but the root biomass was higher in plants grown under severe water deficit conditions compared to control. Photosynthesis, transpiration and stomatal conductance decreased with increased water stress in both cultivars, but the coastal variety showed higher water use efficiency and less discrimination of ¹³C under water deficit. This response was associated with greater root development and a better stomatal opening adjustment, especially in the case of Rainbow. The capacity of Rainbow to increase its osmoregulant content (compounds such as proline, glutamine, glutamate, K and Na) could enable a potential osmotic adjustment in this variety. Moreover, the lower stomatal opening and transpiration rates were also associated with higher leaf ABA concentration values detected in Rainbow. We found negative logarithmic relationships between stomatal conductance and leaf ABA concentration in both varieties, with significant R² values of 0.50 and 0.22 in Rainbow and Illpa, respectively. These moderate-to-medium values suggest that, in addition to ABA signaling, other causes for stomatal closure in quinoa under drought such as hydraulic regulation may play a role. In conclusion, this work showed that two quinoa cultivars use different strategies in the face of water deficit stress, and these prevent decreases in grain yield and quality under drought conditions.

Wheat is a target crop within the food security context. The responses of wheat plants under elevated concentrations of CO₂ (e[CO₂]) have been previously studied; however, few of these studies have evaluated several organs at different phenological stages simultaneously under free-air CO₂ enrichment (FACE) conditions. The main objective of this study was to evaluate the effect of e[CO₂] in two cultivars of wheat (Triumph and Norin), analyzed at three phenological stages (elongation, anthesis, and maturation) and in different organs at each stage, under FACE conditions. Agronomic, biomass, physiological, and carbon (C) and nitrogen (N) dynamics were examined in both ambient CO₂ (a[CO₂]) fixed at 415 µmol mol⁻¹ CO₂ and e[CO₂] at 550 µmol mol⁻¹ CO₂. We found minimal effect of e[CO₂] compared to a[CO₂] on agronomic and biomass parameters. Also, while exposure to 550 µmol mol⁻¹ CO₂ increased the photosynthetic rate of CO₂ assimilation (An), the current study showed a diminishment in the maximum carboxylation (V_c,max) and maximum electron transport (J_max) under e[CO₂] conditions compared to a[CO₂] at physiological level in both cultivars. However, even if no significant differences were detected between cultivars on photosynthetic machinery, differential responses between cultivars were detected in C and N dynamics at e[CO₂]. Triumph showed starch accumulation in most organs during anthesis and maturation, but a decline in N content was observed. Contrastingly, in Norin, a decrease in starch content during the three stages and an increase in N content was observed. The amino acid content decreased in grain and shells at maturation in both cultivars, which might indicate a minimal translocation from source to sink organs. These results suggest a greater acclimation to e[CO₂] enrichment in Triumph than Norin, because both the elongation stage and e[CO₂] modified the source–sink relationship. According to the differences between cultivars, future studies should be performed to test genetic variation under FACE technology and explore the potential of cultivars to cope with projected climate scenarios.