DNA Barcoding is recommended as a tool for identifying and confirming species within the taxonomy framework. The rbcL gene is the barcode DNA for plant species. Ternate Island is one of clove plantation center in North Maluku. The diversity and productivity of cloves on the Ternate island known since in earlier times. This study has a purpose to amplify the rbcL gene in clove plants collected from clove plantations of communities in Ternate island. Isolation of total DNA carried out with using ZymoBiomic (Zymo Research DNA Extraction) Kit from leaves tissue, then DNA specimen is amplified based on rbcL gene with forwarding sequence rbcLaF 5′-ATG TCA CCA CAA ACA GAG ACT AAA GC-3′ and reverse sequence is rbcLaR 5′-GTA AAA TCA AGT CCA CCR CG-3′. The results showed that the specimen was successfully amplified with an amplicon size of 600 bp. Furthermore, BLASTN analysis results note that the sequence has a similarity of 99% with Syzygium cumini vc. J.R. Abbott 23676 (FLAS) ribulose-1,5-bisphosphate carboxylase large subunit (rbcL) gene, but phylogenetically the precise position of sample could not found because the limitation of rbcL gene.

Butterfly pea (Clitoria ternatea L.) is a leguminous plant with several potential health benefits. The scientific name is derived from its origin on Ternate Island, North Maluku. Therefore, this study aimed to analyze the genetic variability in butterfly pea using Intergenic Simple Sequence Repeat (ISSR) markers in North Maluku. Field surveys, collection trips, and habitat studies of butterfly pea plants were conducted on Ternate, Tidore, Halmahera, and Morotai Islands. Genetic diversity was analyzed based on molecular data from the ISSR method. The molecular analysis results obtained using PCR-ISSR on 18 accessions showed a low degree of similarity. Among these, 15 accessions from Ternate, Tidore, Morotai, and Halmahera were in group A, while 3 from Ternate, Tidore, and Halmahera were in group B. All accessions exhibited a genetic similarity level of 0.709, indicating significant diversity. The arrangement among accessions on the dendrogram was similar to the phylogenetic tree, showing separation and spread at 0.608–0.924 based on the Jaccard coefficient. The results suggested that C. ternatea probably originated from Ternate, and subsequently spread to Tidore, Halmahera, and Morotai due to its use as a herbal medicine and ornamental plant. This information could be used as the basis for butterfly pea conservation and cultivation activities in Indonesia, specifically in Ternate Island, North Maluku.

Durian is a tropical fruit that has substantial economic value. One of the durian production centers in North Maluku is Tidore Island. This study’s objective was to identify genetic diversity, taxonomic status, and local durian conservation models in North Maluku. Data collection method with purposive sampling technique. Morphological data, including 142 characters (stems, leaves, flowers, and fruit), were analyzed descriptively and arranged by UPGMA dendrogram with the outgroup (D. kutezensis). Molecular data were obtained by using the PCR RAPD technic. Genetic diversity of local durian Tidore based on morphological characters produced 7 clusters with the closest genetic distance to local durian (R4-R10) with a similarity value of 0.887. Genetic diversity of local durian Tidore based on PCR RAPD technic produced 5 clusters with the closest genetic distance to local durian (R10-R12) with a similarity value of 0.977. The value of Tidore’s local durian dissimilarity is close to 10%; it can be assumed that the genetic diversity of Tidore’s local durian is at the level of intraspecies diversity. Gap analysis recommends that Tidore’s local durian conservation model is appropriate with the durian breeding program through an in-situ selection of superior traits and conservation..

South Halmahera is one of the cocoa-producing centers in North Maluku, but the pulp of cocoa is less noticed and assumed to be not important in commercial purpose and is largely disposed as agroindustry residue. This study aimed to characterize the cocoa pulp from southern Halmahera for bioethanol production. Survey and observation methods were conducted to obtain bioethanol feedstock from cocoa pulp. Cocoa pulps mucilage is a waste that can be processed into bioethanol products with high economic value. The results of this study indicated that theaverage of 100kg of cocoa beans produced 5-8 litters of mucilage juice residues. The results of this study indicated that cocoa pulp from southern Halmahera had the optimum time for the fermentation process for 12 days. The level of alcohol produced by fermentation was 4.85%, and this level was a low category.

Red jabon and gofasa plants has potential as a medicinal plant. One of the potential drugs of these two plants as anti-cholesterol purpose of this study was to identify the red jabon and gofasa plants in Ternate Island using rbcL gene of DNA barcode. The method in this study consists of total DNA isolation using ZymoBiomic (Zymo Research DNA Extraction) Kit, DNA amplification using rbcL gene, sequencing and BLASTn analysis. Based on barcode DNA rbcL, the red jabon plants has similarities 99% Cephalanthus occidentalis, Mitragyna speciosa and Ocherreuclear sp. for gofasa plants has 99% resemblance with Vitex glabrata, Vitex megapotamica, Vitex trifolia and Vitex negundo at NCBI database. The application of rbcL barcode DNA is effective to identify plants at the family level up to the genus.

At present, coconut shell charcoal briquettes are one of the alternative energy sources that are in demand by the export market. The purpose of this research was to produce an alternative briquette product based on local natural materials. Kenari shell is a waste of natural materials or biomass. The characteristics of Kenari shells have the same structure with coconut shell. The pre-design of briquette production of this Kenari shell used pre-treatment process stages, charcoal making, destruction, printing with adhesive variation, drying, and SNI quality of briquette quality analysis. The results of pre-design of bio-briquette production of Kenari shell showed that the application of the method and the basic ingredients of waste could produce the product of charcoal briquettes equal to charcoal briquettes of coconut shell.