Urban structure and landscape cause an artificial temperature increase in cities, known as the urban heat island effect. The magnitude of such urban–rural temperature difference varies in daily and seasonal basis. Daily patterns of urban heat accumulation in Adelaide is under investigation. In this paper, East–West air temperature profile of Adelaide metropolitan area was mapped in 60 journeys alongside a straight cross route connecting Adelaide Hills to the West Beach under clear sky between 26 July and 15 August 2013. The most intense urban–rural temperature differences of 5.9 °C occurred during midnight in Adelaide. However, maximum urban heat variation occurred during the late afternoon when the near-surface urban heat fluctuates by 2 °C between the CBD East and Western Parklands. During summer heatwaves, the afternoon heat stress limits public life vibrancy in Adelaide. Increased urban greenery can facilitate resilience to heat by providing shadow and evaporative cooling. A better understanding of daily urban heat variations and the cooling effect of urban greenery assists urban policy making and public life management in the context of climate change