Rakesh Tiwari

Prof. Rakesh Tiwari

Center for Targeted Drug Delivery, Department of Biomedical and Pharmaceutical Sciences, Chapman University School of Pharmacy, Harry and Diane Rinker Health Science Campus, Irvine, CA 92618, USA
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Research Focus & Metrics

Academic Career Length

15Years (2012-Present)

(Calculated once Scilit publication data is synced: current year − year of first publication + 1)

Representative Publications

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7 October 2020 in Pharmaceuticals
Verified
Journal Article
Open access
PDF file
MSMuhammad Imran Sajid
Muhammad Imran Sajid
MMMuhammad Moazzam
Muhammad Moazzam
SKShun Kato
Shun Kato
YCYeseom Cho
Yeseom Cho
Rakesh TiwariRakesh Tiwari
Rakesh Tiwari
The RNA interference (RNAi) pathway possesses immense potential in silencing any gene in human cells. Small interfering RNA (siRNA) can efficiently trigger RNAi silencing of specific genes. FDA Approval of siRNA therapeutics in recent years garnered a new hope in siRNA therapeutics. However, their therapeutic use is limited by several challenges. siRNAs, being negatively charged, are membrane-impermeable and highly unstable in the systemic circulation. In this review, we have comprehensively discussed the extracellular barriers, including enzymatic degradation of siRNAs by serum endonucleases and RNAases, rapid renal clearance, membrane impermeability, and activation of the immune system. Besides, we have thoroughly described the intracellular barriers such as endosomal trap and off-target effects of siRNAs. Moreover, we have reported most of the strategies and techniques in overcoming these barriers, followed by critical comments in translating these molecules from bench to bedside.

22 July 2019 in Molecular Pharmaceutics
Verified
Journal Article
Closed access
SPShang Eun Park
Shang Eun Park
MSMuhammad Imran Sajid
Muhammad Imran Sajid
Keykavous ParangKeykavous Parang
Keykavous Parang
Rakesh TiwariRakesh Tiwari
Rakesh Tiwari

19 June 2015 in Basic & Clinical Pharmacology & Toxicology
Verified
Journal Article
Closed access
PDF file
MSMeenakshi Sharma
Meenakshi Sharma
MTManisha Tiwari
Manisha Tiwari
Rakesh TiwariRakesh Tiwari
Rakesh Tiwari

28 November 2022 in Journal of Medicinal Chemistry
Verified
Journal Article
Open access
EMEman H. M. Mohammed
Eman H. M. Mohammed
SLSandeep Lohan
Sandeep Lohan
TGTarra Ghaffari
Tarra Ghaffari
SGShilpi Gupta
Shilpi Gupta
Rakesh TiwariRakesh Tiwari
Rakesh Tiwari
Keykavous ParangKeykavous Parang
Keykavous Parang
We designed a library of 24 cyclic peptides containing arginine (R) and tryptophan (W) residues in a sequential manner [RnWn] (n = 2-7) to study the impact of the hydrophilic/hydrophobic ratio, charge, and ring size on the antibacterial activity against Gram-positive and Gram-negative strains. Among peptides, 5a and 6a demonstrated the highest antimicrobial activity. In combination with 11 commercially available antibiotics, 5a and 6a showed remarkable synergism against a large panel of resistant pathogens. Hemolysis (HC50 = 340 μg/mL) and cell viability against mammalian cells demonstrated the selective lethal action of 5a against bacteria over mammalian cells. Calcein dye leakage and scanning electron microscopy studies revealed the membranolytic effect of 5a. Moreover, the stability in human plasma (t1/2 = 3 h) and the negligible ability of pathogens to develop resistance further reflect the potential of 5a for further development as a peptide-based antibiotic.

23 October 2018 in European Journal of Medicinal Chemistry
Verified
Journal Article
Closed access
SDShaban Darwish
Shaban Darwish
NSNeda Sadeghiani
Neda Sadeghiani
STShirley Tong
Shirley Tong
SMSaghar Mozaffari
Saghar Mozaffari
PHParinaz Hamidi
Parinaz Hamidi
Keykavous ParangKeykavous Parang
Keykavous Parang

4 May 2021 in Molecular Pharmaceutics
Verified
Journal Article
Closed access
MSMuhammad Imran Sajid
Muhammad Imran Sajid
MMMuhammad Moazzam
Muhammad Moazzam
YCYeseom Cho
Yeseom Cho
SKShun Kato
Shun Kato
AXAva Xu
Ava Xu
Rakesh TiwariRakesh Tiwari
Rakesh Tiwari