Mixed millet and rice agriculture is a unique agricultural style of China, and is distributed in a broad band between the Yangtze and Yellow River basin. However, the development of this style during the Neolithic-Bronze Age has not been comprehensively clarified, owing to limited archaeobotanical data and imprecise chronology for most of the regions. Here, the Central Plain, a location where mixed agriculture may have first appeared, was selected as the area for research. Phytolith and macrofossil analyses from the Zhuzhai site, together with the accelerator mass spectrometer (AMS) radiocarbon dating of samples, reveal information about the temporal changes of mixed agriculture as well as the domestication and cultivation of crops in this region. The results indicate that mixed millet and rice agriculture formed in the Central Plain about 8000 years ago. Common millet was the principal crop in the Peiligang and Yangshao periods, with the domestication process beginning in the Peiligang period and continuing up to the Shang period, at which time it was replaced by foxtail millet. Foxtail millet may have gone through a significant degree of domestication by ca. 6000 cal. BC, but its domestication process was still unclear. Rice had appeared since the Peiligang period, but its proportions were always low. Rice assigned to the Peiligang and Yangshao Cultures was the domesticated japonica, and its cultivation was always performed in dry field systems through the Neolithic-Bronze time. Within the subsistence economy, mixed agriculture was a minor component during the Peiligang period, but has been dominant since the Yangshao period.

In the last decade, our understanding of rice domestication has improved by new archaeological findings using advanced analytical techniques such as morphological and morphometric analyses on rice grains, spikelet bases and phytoliths, and ancient DNA analysis on rice remains. Previous studies have considered the size of rice bulliform phytoliths as a proxy for tracking the domestication process. These phytoliths are often abundant and well preserved in sediments, and their shape is under the control of numerous genes, which may shift toward larger sizes by genetic mutation in domestication. Therefore, it has been assumed that the bulliforms of domesticated rice are usually larger than those of wild ones; however, morphometric data supporting this assumption are lacking in the literature, thereby requiring additional evidence to test its veracity. In this study, the vertical and horizonal lengths of bulliform phytoliths were measured in four rice species (domesticated Oryza sativa and wild Oryza rufipogon, Oryza officinalis, and Oryza meyeriana) from different regions of southern China. We found that the bulliform morphometric data of wild and domesticated rice overlapped and that there was no statistically significant difference between them. Therefore, bulliform size could not be used as a diagnostic indicator to distinguish domesticated rice from wild species and is a supporting rather than conclusive proxy for determining the domesticated status of rice in archaeological research. We further found that larger rice bulliform sizes likely occurred at the locations with higher temperature, precipitation, and water levels, indicating hydrothermal environment is an alternative factor influencing the size of rice bulliform phytoliths. For further archaeological use of an increasing size trend of bulliform phytoliths to reveal the process of rice domestication, we present some suggestions for controlling the influence of hydrothermal factors. Even so, the combination of bulliform phytolith size with other established criteria is strongly suggested to provide precise identification of wild and domesticated rice in future research.

This study presents new archaeobotanical evidence for agricultural production in the middle Yellow River region during the Yangshao culture period. Phytolith analyses, together with radiocarbon dating of samples from 10 sites in Zhengzhou, showed that common millet and foxtail millet were cultivated with rice in the region around 4000–3000 cal BC. The ratio of crop remains revealed that common millet was dominant in the crop structure. Rice cultivation was no longer confined to large sites situated in the lowlands and began to spread into the hilly lands and small sites. Furthermore, both dryland and wetland systems may have been used for rice cultivation. This pattern of crop production may have been mainly influenced by social background and artificial selection, which overcame the limitation of environmental factors. Such development of Yangshao agriculture facilitated the establishment of an agricultural society during the fourth millennium BC. It also has implications for understanding the reason why the middle Yellow River region (Central Plain) became known as ‘the cradle of Chinese civilization’.

Detailed studies of the long-term development of plant use strategies indicate that plant subsistence patterns have noticeably changed since the Upper Paleolithic, when humans underwent a transitional process from foraging to agriculture. This transition was best recorded in west Asia; however, information about how plant subsistence changed during this transition remains limited in China. This lack of information is mainly due to a limited availability of sufficiently large, quantified archaeobotanical datasets and a paucity of related synthetic analyses. Here, we present a compilation of extensive archaeobotanical data derived from interdisciplinary approaches, and use quantitative analysis methods to reconstruct past plant use from the Upper Paleolithic to Middle Neolithic in China. Our results show that intentional exploitation for certain targeted plants, particularly grass seeds, may be traced back to about 30,000 years ago during the Upper Paleolithic. Subsequently, the gathering of wild plants dominated the subsistence system; however, this practice gradually diminished in dominance until about 6~5 ka cal BP during the Middle Neolithic. At this point, farming based on the domestication of cereals became the major subsistence practice. Interestingly, differences in plant use strategies were detected between north and south China, with respect to (1) the proportion of certain plant taxa in assemblages, (2) the domestication rate of cereals, and (3) the type of plant subsistence practiced after the establishment of full farming. In conclusion, the transition from foraging to rice and millet agriculture in China was a slow and long-term process spanning 10s of 1000s of years, which may be analogous to the developmental paths of wheat and barley farming in west Asia.