Purple-fleshed potatoes (PFP) are varieties of Solanum tuberosum L., which recently have been recorded to be more and more cultivated and consumed in all European countries, including Romania, as they are promoted for their content in bioactive compounds and benefits to human health. This paper presents a micropropagation protocol study for PFP varieties already traded into the Romanian market, namely Blue Danube (BD), Salad Blue (SB), Violet Negretin (VN), and Violet Queen (VQ). These varieties were tested for in vitro micropropagation also considering asepsis, initiation, callus formation, and microtuberization. To establish the optimum asepsis treatment, a preliminary experiment was performed and, the best results were obtained by using 70% EtOH (1 min) followed by 20% (v/v) Domestos^® (20 min). The MS formula (Murashige and Skoog 1962) was tested as the basic culture medium without growth regulators for all tested stages except for callus initiation and its further multiplication stages. The effect of glycine on direct organogenesis and shoot multiplication was evaluated for propagated micro-cuttings. We emphasize that the addition of glycine at a concentration of 15 mg/L to the culture medium induced a better plantlet vigor for all four varieties. Regarding the indirect organogenesis, culture medium supplemented with NAA (5.00 mg/L), GA₃ (1.00 mg/L), TDZ (1.00 mg/L) and glycine (15.00 mg/L) induced the best results for shoot cluster regeneration as well as turning of white callus from control to purple callus. Further, the microtuberization was successfully produced when sucrose was supplemented at 8% (w/v) into the culture medium. Among all four tested PFP varieties, SB has proven to give the best results regarding the adaptability for in vitro cultivation.

The maintenance of healthy status of a didactic apple tree orchard is very important for teaching students as well as for research purposes. Fungal diseases represent a significant threat to apple tree orchards, affecting both yield and fruit quality. The scope of this study was to evaluate the phytosanitary status of two apple orchards in the Rusciori Didactic and Research Farm, Sibiu County, for 2024 season, focusing on fruit brown rot caused by Monilinia fructigena (Aderhold & Ruhland) Honey.Thus, an old apple orchard (i.e. planted in 1960) and a new one (i.e. planted in 2019) were investigated. The analysis of our results proved that the older orchard was more susceptible, with an infection rate of 43.58%, an intensity of 11.21%, and a damage degree of 4.39%, compared to the new orchard where accordingly were recorded 15.40%, 2.86%, and 0.28%.Our preliminary study results support the idea that the age of apple tree can be a determinant factor for susceptibility to these diseases, that can be enhanced by some relevant environmental factors. Thus, it can be considered that high humidity, optimal temperatures range (15–25°C), and wind, may favour pathogen development, especially during the fruit ripening period. Preventive treatments applied within the framework of integrated disease management effectively reduced infection rates and consequently economic losses, particularly for the young orchard. Our preliminary study results highlight that the old orchard may act as primary inoculum sources, emphasizing the importance of cultural hygiene and timely interventions.

This paper explores the interactions between systematic cadastre, land legislation, and their economic implications for the land market, focusing on a case study of Hoghilag Commune, Sibiu County, Romania.The research integrates theoretical and empirical perspectives to highlight how cadastral registration and land law frameworks influence agricultural land value and leasing practices. The study is grounded in Romanian national legislation, the methodology of the State Domains Agency (ADS), and statistical data provided by the National Institute of Statistics (INS), complemented by previous research on land leasing and systematic cadastre. Mathematical models are employed to calculate rent values and economic losses, correlated with wheat price quotations and the euro/leu exchange rate.The findings emphasize the importance of accurate cadastral data and coherent legal mechanisms in ensuring transparency and efficiency in the agricultural land market, as well as in supporting informed decision-making for local and national land management policies.