Cholesterol accumulation in macrophages leads to the formation of foam cells and increases the risk of developing atherosclerosis. We have verified whether hydroxytyrosol (HT), a phenolic compound with anti-inflammatory and antioxidant properties, can reduce the cholesterol build up in THP-1 macrophage-derived foam cells. We have also investigated the potential mechanisms. Oil Red O staining and high-performance liquid chromatography (HPLC) assays were utilized to detect cellular lipid accumulation and cholesterol content, respectively, in THP-1 macrophages foam cells treated with HT. The impact of HT on cholesterol metabolism-related molecules (SR-A1, CD36, LOX-1, ABCA1, ABCG1, PPARγ and LRX-α) in foam cells was assessed using real-time PCR (RT-qPCR) and Western blot analyses. Finally, the effect of HT on the adhesion of THP-1 monocytes to human vascular endothelial cells (HUVEC) was analyzed to study endothelial activation. We found that HT activates the PPARγ/LXRα pathway to upregulate ABCA1 expression, reducing cholesterol accumulation in foam cells. Moreover, HT significantly inhibited monocyte adhesion and reduced the levels of adhesion factors (ICAM-1 and VCAM-1) and pro-inflammatory factors (IL-6 and TNF-α) in LPS-induced endothelial cells. Taken together, our findings suggest that HT, with its ability to interfere with the import and export of cholesterol, could represent a new therapeutic strategy for the treatment of atherosclerotic disease.

Living organisms do not disregard the laws of thermodynamics and must therefore consume energy for their survival. In this way, cellular energy exchanges, which aim above all at the production of ATP, a fundamental molecule used by the cell for its metabolisms, favor the formation of waste products that, if not properly disposed of, can contribute to cellular aging and damage. Numerous genes have been linked to aging, with some favoring it (gerontogenes) and others blocking it (longevity pathways). Animal model studies have shown that calorie restriction (CR) may promote longevity pathways, but given the difficult application of CR in humans, research is investigating the use of CR-mimetic substances capable of producing the same effect. These include some phytonutrients such as oleuropein, hydroxytyrosol, epigallo-catechin-gallate, fisetin, quercetin, and curcumin and minerals such as magnesium and selenium. Some of them also have senolytic effects, which promote the apoptosis of defective cells that accumulate over the years (senescent cells) and disrupt normal metabolism. In this article, we review the properties of these natural elements that can promote a longer and healthier life.

The present study aimed to investigate how playing positions differ in specific body composition variables in professional soccer players with respect to specific field zones and tactical lines. Five hundred and six Serie A and B professional soccer players were included in the study and analyzed according to their playing positions: goalkeepers (GKs), central backs (CBs), fullbacks (FBs), central midfielders (MIDs), wide midfielders (WMs), attacking midfielders (AMs), second strikers (SSs), external strikers (ESs), and central forwards (CFs), as well as their field zones (central and external) and tactical lines (defensive, middle, and offensive). Anthropometrics (stature and body mass) of each player were recorded. Then, body composition was obtained by means of bioelectric impedance analysis (BIA). GKs and CFs were the tallest and heaviest players, with no differences from each other. Likewise, GKs and CFs, along with CBs, were apparently more muscular (for both upper and lower limbs) and fatter at the same time compared with the other roles. Overall, players of the defensive line (CBs and FBs), along with those playing in central field zones (CBs, MIDs, AMs, SSs, and CFs), were significantly (p < 0.05) superior in almost all anthropometric and body composition variables than those of middle and offensive line and external zones, respectively.

Ankle-brachial index and arterial stiffness are associated with leg function in the elderly and in patients with peripheral arterial disease. Little is known about the meaning of these parameters in young and trained subjects and how they are related to physical performance. The main objective was to evaluate the mediating role of arterial stiffness and ankle-brachial index in physical performance. In a cross-sectional, case-control study, 240 male athletes were consecutively enrolled from the Laboratory of Cardiology and Sports Medicine, “G. d’Annunzio” University (Italy). All the subjects underwent the examination protocol for the annual medical evaluation for sport participation. Soccer (football) players compared to runners showed a lower level of ankle-brachial index, higher arterial stiffness, and lower systolic and diastolic blood pressure. In the treadmill stress test, soccer players compared to runners showed a greater maximal aerobic capacity. Differences in cardiovascular performance between soccer players and runners were mediated by better arterial stiffness and low level of ankle-brachial index; the estimated effect was 0.11 ± 0.05 and 0.24 ± 0.06, respectively. Vigorous strength training drops blood pressure and increases arterial stiffness. Taken together, our findings would seem to suggest that ABI and CAVI could be used as markers for athletes’ performance.

Eating Disorders represent one of the fastest-growing mental health problems in the world. While a diagnosis of an eating disorder can only be made after careful psychiatric evaluation, the detection of “at-risk” individuals can be conducted through screening programs that use tools such as the Eating Attitude Test (EAT-26), a test used to measure the symptoms and concerns characteristic of eating disorders. This observational study was designed to evaluate the presence of at-risk individuals, using the EAT-26, among young athletes in “lean sports”, young athletes in non-”lean sports”, young non-athlete sportspeople, and young people who did not practice any sports. In addition, the presence of a correlation between the risk of developing eating disorders, gender, and age was also investigated. The results identified a prevalence of risk of 13% among study participants. None of the subjects who did not engage in any physical activity obtained a test score that could be considered at risk. Furthermore, no correlation was found between gender and the scores obtained. These results should be taken into consideration to emphasize the importance of nutritional intervention by expert and qualified personnel within various sports societies, in order to prevent the development of these pathologies and at the same time increase and improve performance.