Microfluidics is very crucial in lab-on-a-chip systems for carrying out operations in a large-scale laboratory environment on a single chip. Microfluidic systems are miniaturized devices in which the fluid behavior and control can be manipulated on a small platform, with surface forces on the platform being greater than volumetric forces depending on the test method used. In recent years, paper-based microfluidic analytical devices (μPADs) have been developed to be used in point-of-care (POC) technologies. μPADs have numerous advantages, including ease of use, low cost, capillary action liquid transfer without the need for power, the ability to store reagents in active form in the fiber network, and the capability to perform multiple tests using various measurement techniques. These benefits are critical in the advancement of paper-based microfluidics in the fields of disease diagnosis, drug application, and environment and food safety. Cancer is one of the most critical diseases for early detection all around the world. Detecting cancer-specific biomarkers provides significant data for both early diagnosis and controlling the disease progression. μPADs for cancer biomarker detection hold great promise for improving cure rates, quality of life, and minimizing treatment costs. Although various types of bioanalytical platforms are available for the detection of cancer biomarkers, there are limited studies and critical reviews on paper-based microfluidic platforms in the literature. Hence, this article aims to draw attention to these gaps in the literature as well as the features that future platforms should have.

Two‐dimensional (2D) borophene, a single‐atom‐thick allotrope of boron, exhibits exceptional conductivity, anisotropy, and chemical reactivity, yet very little is known about its electrochemical properties. Here, we delineate its enzymatic and electrochemical behavior under biologically relevant redox conditions. Spectroscopic and microscopic studies reveal concentration‐dependent degradation of borophene by hydrogen peroxide (H ₂ O ₂ ), yielding boronic acids, confirmed by a curcumin‐rosocyanine assay. Enzymatic cascades employing glucose oxidase and horseradish peroxidase establish borophene as both an electrocatalyst and a chemically responsive transducer, generating dual colorimetric and electrochemical outputs. Electroanalytical measurements (cyclic voltammetry, chronoamperometry, and differential pulse voltammetry) show that borophene efficiently wires peroxidase reactions by sensitizing H ₂ O ₂ detection through borophene–HRP interfaces. In contrast, glucose sensing displays diminished currents due to reactive oxygen species–mediated passivation. These results position borophene as a unique platform for catalytic wiring of enzyme cascades in which ROS flux dynamically regulates signal output, enabling transient, self‐reporting biosensors and motivating stabilization strategies for long‐term bioelectronic integration.

Borophene (BPH), a monolayer boron allotrope, exhibits outstanding electrical and mechanical properties but remains intrinsically constrained by its excessive susceptibility to oxidative degradation in aqueous and physiological environments. This study demonstrates that protein-assisted exfoliation using human serum albumin (HSA) establishes a dynamic biointerfacial sheath (BIS) that decouples environmental stability from intracellular degradation. HSA forms a robust BIS via multivalent hydrophobic, electrostatic, and thiol-mediated interactions, driving exfoliation into ultrathin nanosheets while suppressing air- and serum-induced oxidation. Proteinized BPH (PBPH) shows enhanced extracellular colloidal and chemical stability; however, upon cellular uptake, the same interface promotes rapid reactive oxygen species-driven degradation, leading to lattice fragmentation, loss of crystallinity, and conversion into soluble boronate species. Multimodal analyses confirm accelerated degradation kinetics and environmental stability of PBPH. These results highlight the protein corona as an active regulator of nanomaterial fate, enabling programmable decoupling of extracellular persistence from intracellular clearance in oxidation-prone two-dimensional systems.