Peony is a famous ornamental and medicinal plant in China, and peony hybrid breeding is an important means of germplasm innovation. However, research on the genome of this species is limited, thereby hindering the genetic and breeding research on peony. In the present study, simple sequence repeat (SSR) locus analysis was performed on expressed sequence tags obtained by the transcriptome sequencing of Paeonia using Microsatellite software. Primers with polymorphism were obtained via polymerase chain reaction amplification and electrophoresis. As a result, a total of 86,195 unigenes were obtained by assembling the transcriptome data of Paeonia. Functional annotations were obtained in seven functional databases including 49,172 (Non-Redundant Protein Sequence Database: 57.05%), 38,352 (Nucleotide Sequence Database: 44.49%), 36,477 (Swiss Prot: 42.32%), 38,905 (Clusters of Orthologous Groups for Eukaryotic Complete Genomes: 45.14%), 37,993 (Kyoto Encyclopedia of Genes and Genomes: 44.08%), 26,832 (Gene Ontology: 31.13%) and 37,758 (Pfam: 43.81%) unigenes. Meanwhile, 21,998 SSR loci were distributed in 17,567 unigenes containing SSR sequences, and the SSR distribution frequency was 25.52%, with an average of one SSR sequence per 4.66 kb. Mononucleotide, dinucleotide, and trinucleotide were the main repeat types, accounting for 55.74%, 25.58%, and 13.21% of the total repeat times, respectively. Forty-five pairs of the 100 pairs of primers selected randomly could amplify clear polymorphic bands. The polymorphic primers of these 45 pairs were used to cluster and analyze 16 species of peony. The new SSR molecular markers can be useful for the study of genetic diversity and marker-assisted breeding of peony.

Light is one of the most important environmental factors influencing plant growth and development. Light-emitting diodes (LEDs) are are a promising alternative to conventional light sources for plant tissue cultures. Combined red (R) and blue (B) LEDs are suitable for the production of many plant species. However, few studies have examined the effects of different R:B ratios on Gerbera jamesonii under controlled conditions. G. jamesonii plantlets were grown in controlled conditions under different ratios of discreet and blue light wavelengths provided by programmable LEDs. The effects of the different LED treatments on various morphological indices, photosynthetic parameters, soluble sugar and protein contents, and antioxidant enzyme activities were analyzed. Growth under monochromatic red light resulted in the tallest plantlets and longest stomata, and most promoted soluble content and intercellular CO 2 concentration (C i). Growth under monochromatic blue light promoted the highest levels of soluble protein, chlorophyll a (chlorophyll a (Chl a), and chlorophyll b (chlorophyll b (Chl b). An R:B ratio of 6:4 was the ideal LED treatment for enhancing leaf length, root length, and peroxidase activity. Growth under R:B = 7:3 promoted the number of leaves, leaf width, number of roots, superoxide dismutase, and catalase activities, chlorophyll synthesis, and the accumulation of Chl a and Chl b and led to the highest fluorescence parameters. With the exception of three physiological indicators, other indicators growth under R:B = 7:3 were better than under other tested ratios, implying that R:B = 7:3 represents the ideal LED treatment for improving G. jamesonii growth and photosynthetic activities under controlled conditions.

Floral longevity (FL) is an important floral functional trait which is critical for flowering plants. FL shows great diversity among angiosperms; however, there is limited information on the mechanisms that influence differences in floral longevity, especially the relationship between petal anatomical traits and floral longevity. We aimed to examine (1) the relationships between petal anatomical traits and FL in tree peony cultivars and (2) the petal anatomical characteristics of longer FL cultivars. Eleven traits of six tree peony cultivars with different FL were investigated, including six water conservation traits (petal thickness, cuticle thickness, number of cell layers, mesophyll thickness, adaxial epidermis thickness and abaxial epidermis thickness), three water supply traits (vein density, number of xlylem vessels and xylem vessel diameter), petal fresh mass and petal dry mass across cultivars. There are significant differences in traits related to water conservation and water supply ability of tree peonies with different FL. Tree peony cultivars with long FL were characterized by the thicker Mesophyll, cuticles, adaxial and abaxial epidermis of the petals. There was a positive correlation between FL and vessel number and vessel diameter. These results suggest that the ability to retain water in flowers is associated with floral longevity. Petal traits related to water conservation and supply, including vein densities, mesophyll thickness, and epidermis thickness, are beneficial for prolonging the flower longevity in tree peonies.

Anthurium andreanum was one of the best indoor ornamental plants. Two cultivars of Anthurium andreanum (Pink Champion, Alabama) were used to investigate the effects of light quality on physiological and biochemical indexes. There were six different light quality treatments: Fluorescent Daylight Lamp (CK), and RB (100% Blue, 60% R + 40% B, 70% R + 30% B, 80% R + 20% B, 100% Red) provided by light emitting diodes (LED). The results showed that blue light was beneficial to shoot growth and dry matter accumulation, photosynthetic rate, soluble sugar, and POD activities. Red light was beneficial for the synthesis and accumulation of soluble protein, and could promote root growth. ‘Pink Champion’ and ‘Alabama’ obtained the relatively better morphological parameters, chlorophyll contents, photosynthetic parameters, and antioxidant enzyme activities in 7:3 and 6:4 treatments. The antioxidant enzyme (POD, SOD) activities under composite light of red and blue treatments were better than that of monochromatic red, blue light treatments and CK on the whole. Comprehensive evaluation showed that the treatment of 7:3 was a suitable light environment indoors and could be used as the preferred light quality ratio in the production and application of Anthurium andreanum.