Monitoring land cover changes in protected areas is crucial to control the conservation efficiency of biodiversity and natural ecosystem conditions, especially in Ecuador, one of the most megadiverse countries in the world. Therefore, the purpose of the present study has been to estimate spatiotemporal changes in the landscape and the level of fragmentation using remote sensing in Llanganates National Park (PNL), a protected area in central Andean Ecuador. To obtain land cover, satellite images were processed using the Maximum Likelihood Classification (MLC) algorithm. After statistical analysis, it was encountered that there is no significant difference in land cover change between the years 1991 to 2016 nor among the three watersheds, while the level of fragmentation in the PNL is low. Land cover changes in the study area are not evident, as it is a protected area where ecosystems are usually expected to maintain their initial conditions over time. Therefore, with these results it has been concluded that the biodiversity and landscape conservation processes in the PNL are effective.