Cutaneous wounds affect millions of people every year. Vascularization and blood oxygen delivery are critical bottlenecks in wound healing, and understanding the spatiotemporal dynamics of these processes may lead to more effective therapeutic strategies to accelerate wound healing. In this work, we applied multi-parametric photoacoustic microscopy (PAM) to study vascular adaptation and the associated changes in blood oxygen delivery and tissue oxygen metabolism throughout the hemostasis, inflammatory, proliferation, and early remodeling phases of wound healing in mice with skin puncture wounds. Multifaceted changes in the vascular structure, function, and tissue oxygen metabolism were observed during the 14-day monitoring of wound healing. On the entire wound area, significant elevations of the arterial blood flow and tissue oxygen metabolism were observed right after wounding and remained well above the baseline over the 14-day period. On the healing front, biphasic changes in the vascular density and blood flow were observed, both of which peaked on day 1, remained elevated in the first week, and returned to the baselines by day 14. Along with the wound closure and thickening, tissue oxygen metabolism in the healing front remained elevated even after structural and functional changes in the vasculature were stabilized. On the newly formed tissue, significantly higher blood oxygenation, flow, and tissue metabolism were observed compared to those before wounding. Blood oxygenation and flow in the new tissue appeared to be independent of when it was formed, but instead showed noticeable dependence on the phase of wound healing. This PAM study provides new insights into the structural, functional, and metabolic changes associated with vascular adaptation during wound healing and suggests that the timing and target of vascular treatments for wound healing may affect the outcomes.

A performance optimization design for a high-speed fiber Bragg grating (FBG) interrogation system based on a high-speed distributed feedback (DFB) swept laser is proposed. A time-division-multiplexing sensor network with identical weak FBGs is constituted to realize high-capacity sensing. In order to further improve the multiplexing capacity, a waveform repairing algorithm is designed to extend the dynamic demodulation range of FBG sensors. It is based on the fact that the spectrum of an FBG keeps stable over a long period of time. Compared with the pre-collected spectra, the distorted spectra waveform are identified and repaired. Experimental results show that all the identical weak FBGs are distinguished and demodulated at the speed of 100 kHz with a linearity of above 0.99, and the range of dynamic demodulation is extended by 40%.

An anti-noise interrogation technique for ultra-weak fiber Bragg grating (UWFBG)-based distributed acoustic sensing (DAS) systems is proposed and demonstrated using double-pulse-based time-domain adaptive delay interference. This technique breaks the limitation that the optical path difference (OPD) between the two arms of the interferometer should be completely matched with the entire OPD between the adjacent gratings in the traditional single-pulse system. The length of the delay fiber in the interferometer can be reduced, and the double-pulse interval can adapt flexibly to the UWFBG array with different grating spacing. The acoustic signal is restored accurately when the grating spacing is 15 m or 20 m by the time-domain adjustable delay interference. Moreover, the noise induced by the interferometer can be suppressed significantly as compared to using a single pulse, and above 8-dB signal-to-noise ratio (SNR) enhancement can be obtained without any extra optical devices when the noise frequency and the vibration acceleration are below 100 Hz and 0.1 m/s², respectively.

Crack generation starts at the crack tip, which bears the highest stress concentration. Under further stress, the crack propagates and leads to severe structural damage. To avoid such damage, the identification of the crack tips, and monitoring of the surrounding stress and strain fields, are very important. In this work, the location of, and strain distribution monitoring around, the crack tip are achieved using a dense ultra-short (DUS) fiber Bragg grating (FBG) array together with an improved optical frequency domain reflectometry (OFDR) interrogator. The adjacent grating interference correlation algorithm helps overcome the limitation on the demodulation precision, which is imposed by the inherently broad reflection spectra of individual ultra-short gratings. High spatial resolution measurement of the strain profile around the crack tip is performed at different levels of induced strain. Furthermore, the vertical-crossed layout is adopted to avoid the omission of cracks, which usually occurs in the case of the one direction layout. We achieve 1 mm spatial resolution and 7.5 m detection distance. Location of a single crack, multiple cracks, and an oblique crack was realized experimentally by locating the crack tips. The experimental results are consistent with the theoretical analysis, verifying the feasibility of the DUS-FBG system for high-density distributed crack tip sensing.

Fourier domain mode-locking has been used in fiber Bragg grating interrogation for high-speed and long-distance measurement. But the delays induced by long fiber between multisensors and interrogation systems result in nonneglected wavelength error in this high-speed wavelength-swept system. More seriously, the cross-sweep cycle and different distance delays may cause the delay-disorder problem which makes the system unable to determine the order of the delayed peaks and unable to address the location of sensors with corresponding wavelength-encoding characteristics. This paper proposes a novel delayed-peak recognition and calibration method in which the laser works at four different scanning rates. We confirm the relationship between delayed peaks and sensors with recognition parameters at two low rates and eliminate wavelength error at two high rates. Experimental results show that this method recognizes the delayed peaks correctly and calibrates wavelength accurately with error below 10 pm.