The sudden outbreak of monkeypox in 2022 suggests the importance of developing a rapid but sensitive virus detection technology. Herein, we report a colorimetric/surface-enhanced Raman scattering (SERS) dual-signal co-enhanced immunochromatographic assay (ICA) for the flexible, ultrasensitive, and accurate detection of monkeypox virus (MPXV) in various complex samples. A thickness-controlled polyethyleneimine interlayer (1 nm) is coated onto two-dimensional molybdenum disulfide (MoS₂) nanosheet to enable the electrostatic adsorption of two layers of dense 30 nm AuNPs, which not only improves colorimetric ability but also creates numerous efficient SERS hotspots. Moreover, the SERS activity of film-like dual-signal tag (MoS₂@Au-Au) is drastically enhanced by combining the chemical enhancement effect of MoS₂ sheets and the electromagnetic enhancement effect of Au-Au hotspots. The introduction of MoS₂@Au-Au greatly broadens the application range of existing ICA methods, in which the colorimetric signal supports the quick identification of the target virus and the SERS signal allows the quantitative detection of MPXV with detection limits of as low as 0.2 and 0.002 ng/mL. Given its rapid detection ability (< 20 min), high accuracy in real samples (RSD < 9.89 %), and superior sensitivity than traditional AuNP-based colorimetric ICA (> 500 times), the proposed assay has great potential for field application.

This review describes recent advances in the use of magnetic-plasmonic particles (MPPs) for bacteria detection by Surface-Enhanced Raman Scattering (SERS). Pathogenic bacteria pollution has always been a major threat to human health and safety. SERS spectroscopy has emerged as a powerful and promising technique for sensitive and selective detection of pathogen bacteria. MPPs are considered as a versatile SERS platform for their excellent plasmonic properties and good magnetic responsiveness. Improved preparation method and typical characterization technique of MPPs are introduced, focusing on the thin and continuous metallic shell covering process. Consequently, the SERS-based sensing methods for bacteria identification were discussed, including the label-free and label-based methods. Finally, an overview of the current state of the field and our perspective on future development directions are given.

An ultrasensitive LFIA based on multilayered fluorescent nanofilm-guided signal amplification was developed for the rapid and quantitative determination of Salmonella typhimurium in complex food samples.