Plant-derived exosome-like nanoparticles (PDENs) comprise various bioactive biomolecules. As an alternative cell-free therapeutic approach, they have the potential to deliver nano-bioactive compounds to the human body, and thus lead to various anti-inflammatory, antioxidant, and anti-tumor benefits. Moreover, it is known that Indonesia is one of the herbal centers of the world, with an abundance of unexplored sources of PDENs. This encouraged further research in biomedical science to develop natural richness in plants as a source for human welfare. This study aims to verify the potential of PDENs for biomedical purposes, especially for regenerative therapy applications, by collecting and analyzing data from the latest relevant research and developments.

A comprehensive understanding of the complex physiological and pathological processes associated with alveolar bones, their responses to different therapeutics strategies, and cell interactions wit... Plain language summary Bone-related issues have been widely focused on in the field of dentistry due to oral cancers, trauma, injuries and the high incidence of periodontitis (a serious gum infecti... Tweetable abstract Monolayer cell cultures and animal studies cannot reflect real time microenvironment of the diseased sites. A microfluidic organ-on-a-chip technology relevant to dental and bone ... Graphical abstract

This research was aimed at investigating the effects of various concentrations of Euphorbia hirta nanoextract as a piezoelectric scaler coolant on gingivitis healing in a Wistar rat model. A piezoelectric ultrasonic scaler coolant was made from E. hirta nanoextract through ionic gelation. Experiments were conducted in 45 adult male Wistar rats divided into three groups treated with E. hirta nanoextract coolant (25%, 30%, and 35% concentrations), and negative and positive control groups. A silk ligature was used to trap debris and induce gingivitis in the maxillary incisors of the rats. Scaling was conducted with a piezoelectric ultrasonic scaler after the respective treatment for each group. Data were collected on days 3, 5, 7, 14, and 21 after treatment. Observations were collected with an Optilab® camera at 400× magnification. Angiogenesis and neutrophil data were analyzed with two-way analysis of variance (ANOVA) and post hoc Duncan tests at a 95% significance level. Use of E. hirta nanoextract as a piezoelectric ultrasonic coolant accelerated gingivitis healing in Wistar rats, particularly at a 25% concentration. Two-way ANOVA indicated a significant difference in angiogenesis and neutrophil counts between the control group and each treatment group (p < 0.05). Duncan's post-hoc test showed significant differences in mean neutrophil numbers and angiogenesis among groups on days 3, 5, 7, 14, and 21. The group treated with 25% nanoextract concentration showed no significant differences with respect to the positive control group. Use of E. hirta nanoextract as a piezoelectric ultrasonic coolant had good therapeutic results in promoting gingivitis healing. E. hirta nanoextract may potentially resolve inflammation in gingivitis by modulating neutrophils and angiogenesis.