Anggraeni R, et al. Clin Exp Vaccine Res. 2022 Sep;11(3):235-248. https://doi.org/10.7774/cevr.2022.11.3.235

It would certainly be an advantage of resin-modified glass ionomer cement (RMGIC) if it can possess bioactivity. However, research related to that is still very limited. Hybridization of RMGIC was predicted to increase mechanical properties and resistance to disintegration, and low content of polyacrylic acid induces cement bioactivity. This study investigated the effect of BAG obtained from the CaSiO3–Ca3(PO4)2 system on RMGIC bioactivity. BAG samples containing 10%, 15%, and 20% P2O5 (denoted as “CSP10,” “CSP15,” and “CSP20,” respectively) were used to modify RMGIC powder, and apatite wollastonite was used for comparison. Surface bioactivity was assessed using XRD pattern, infrared spectroscopy, and SEM microstructure after specimen immersion in simulated body fluid (SBF). Contents of Ca, P, F, Sr, and Al were measured in the remaining SBF. Cell attachment, proliferation, and differentiation on the RMGIC containing BAG were evaluated and compared with those on the RMGIC without BAG. Sr and Al analyses revealed that the addition of BAG may not influence the matrix stability of the cement. Moreover, the addition of BAG was a positive factor indicating excellent ion exchange in SBF and spontaneous growth of apatite by consuming the Ca and P ions in the surrounding fluid. Osteoblast differentiation was higher on the four types of bioactive cements than on the RMGIC without BAG. The results of these studies provide novel insights into the development of a new generation of osteoconductive biomedical materials.

A comprehensive understanding of the complex physiological and pathological processes associated with alveolar bones, their responses to different therapeutics strategies, and cell interactions wit... Plain language summary Bone-related issues have been widely focused on in the field of dentistry due to oral cancers, trauma, injuries and the high incidence of periodontitis (a serious gum infecti... Tweetable abstract Monolayer cell cultures and animal studies cannot reflect real time microenvironment of the diseased sites. A microfluidic organ-on-a-chip technology relevant to dental and bone ... Graphical abstract

Bone infections are a key health challenge with dramatic consequences for affected patients. In dentistry, periodontitis is a medically compromised condition for efficient dental care and bone grafting, the success of which depends on whether the surgical site is infected or not. Present treatments involve antibiotics associated with massive bacterial resistance effects, urging for the development of alternative antibacterial strategies. In this work, we established a safe-by-design bone substitute approach by combining bone-like apatite to peroxide ions close to natural in vivo oxygenated species aimed at fighting pathogens. In parallel, bone-like apatites doped with Ag⁺ or co-doped Ag⁺/peroxide were also prepared for comparative purposes. The compounds were thoroughly characterized by chemical titrations, FTIR, XRD, SEM, and EDX analyses. All doped apatites demonstrated significant antibacterial properties toward four major pathogenic bacteria involved in periodontitis and bone infection, namely Porphyromonas gingivalis (P. gingivalis), Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans), Fusobacterium nucleatum (F. nucleatum), and S. aureus. By way of complementary tests to assess protein adsorption, osteoblast cell adhesion, viability and IC₅₀ values, the samples were also shown to be highly biocompatible. In particular, peroxidated apatite was the safest material tested, with the lowest IC₅₀ value toward osteoblast cells. We then demonstrated the possibility to associate such doped apatites with two biocompatible polymers, namely gelatin and poly(lactic-co-glycolic) acid PLGA, to prepare, respectively, composite 2D membranes and 3D scaffolds. The spatial distribution of the apatite particles and polymers was scrutinized by SEM and µCT analyses, and their relevance to the field of bone regeneration was underlined. Such bio-inspired antibacterial apatite compounds, whether pure or associated with (bio)polymers are thus promising candidates in dentistry and orthopedics while providing an alternative to antibiotherapy.

Respiratory infections caused by coronaviruses (CoVs) have become a major public health concern in the past two decades as revealed by the emergence of SARS-CoV in 2002, MERS-CoV in 2012, and SARS-CoV-2 in 2019. The most severe clinical phenotypes commonly arise from exacerbation of immune response following the infection of alveolar epithelial cells localized at the pulmonary blood-air barrier. Preclinical rodent models do not adequately represent the essential genetic properties of the barrier, thus necessitating the use of humanized transgenic models. However, existing monolayer cell culture models have so far been unable to mimic the complex lung microenvironment. In this respect, air-liquid interface (ALI) models, tissue engineered models, and organ-on-a-chip systems, which aim to better imitate the infection site microenvironment and microphysiology, are being developed to replace the commonly used monolayer cell culture models, and their use is becoming more widespread every day. On the other hand, studies on the development of nanoparticles that mimic respiratory viruses, and those nanoparticles used in therapy are progressing rapidly. The first part of this review describes in vitro models that mimic the blood-air barrier, the tissue interface that plays a central role in COVID-19 progression. In the second part of the review, nanoparticles mimicking the virus and/or designed to carry therapeutic agents are explained and exemplified.